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dc.creatorColić, M
dc.creatorStojić-Vukanić, Zorica
dc.creatorPavlović, B
dc.creatorJandrić, Dušan
dc.creatorStefanoska, I
dc.date.accessioned2019-09-02T10:56:59Z
dc.date.available2019-09-02T10:56:59Z
dc.date.issued2003
dc.identifier.issn0009-9104
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/433
dc.description.abstractWe have studied the effect of mycophenolate mofetil (MMF), a new drug used in prevention of transplant rejection, on differentiation, maturation and allostimulatory activity of human monocyte-derived dendritic cells (MDDC). MDDC were generated in vitro with granulocyte macrophage-colony stimulating factor (GM-CSF) and interleukin (IL)-4 in the presence or absence of MMF. MMF reduced the number of immature MDDC in culture, dose-dependently, by inducing apoptosis and inhibited their stimulatory activity on allogeneic lymphocytes. These changes correlated with down-regulation of costimulatory and adhesion molecules such as CD40, CD54, CD80 and CD86. No differences were observed in mannose receptor (MR)-mediated endocytosis, measured by the uptake of fluorescein isothiocyanate (FITC)-dextran. MDDC differentiated in the presence of MMF showed significantly reduced maturation upon stimulation with lipopolysaccharide, as judged by lower expresson of CD83 and co-stimulatory molecules, lower production of tumour necrosis factor (TNF)-alpha, IL-10, IL-12 and IL-18 as well as lower stimulation of alloreactive T cells including naive CD4(+) CD45RA(+)T cells. In contrast, MDDC matured in the presence of MMF showed a more marked decrease in the FITC-dextran uptake than mature MDDC cultivated without MMF and the phenomenon correlated with down-regulation of the MR expression. These results suggest that MMF impairs differentiation, maturation and function of human MDDC in vitro, which is an additional mechanism of its immunosuppressive effect.en
dc.publisherBlackwell Publishing Ltd, Oxford
dc.rightsopenAccess
dc.sourceClinical and Experimental Immunology
dc.subjectalloreactivityen
dc.subjectcytokine productionen
dc.subjectendocytosisen
dc.subjecthuman dendritic cellsen
dc.subjectmycophenolate mofetilen
dc.subjectphenotypeen
dc.titleMycophenolate mofetil inhibits differentiation, maturation and allostimulatory function of human monocyte-derived dendritic cellsen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractПавловић, Б; Стефаноска, И; Јандрић, Душан; Стојић-Вуканић, Зорица; Цолић, М;
dc.citation.volume134
dc.citation.issue1
dc.citation.spage63
dc.citation.epage69
dc.citation.other134(1): 63-69
dc.citation.rankM22
dc.identifier.wos000185345200011
dc.identifier.doi10.1046/j.1365-2249.2003.02269.x
dc.identifier.pmid12974756
dc.identifier.scopus2-s2.0-19244387277
dc.identifier.fulltexthttp://farfar.pharmacy.bg.ac.rs//bitstream/id/1965/431.pdf
dc.identifier.rcubconv_1421
dc.type.versionpublishedVersion


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