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Encapsulation of cannabidiol in oil-in-water nanoemulsions and nanoemulsion-filled hydrogels: A structure and biological assessment study
dc.creator | Demisli, Sotiria | |
dc.creator | Galani, Eleni | |
dc.creator | Goulielmaki, Maria | |
dc.creator | Kyrilis, Fotios | |
dc.creator | Ilić, Tanja | |
dc.creator | Hamdi, Farzad | |
dc.creator | Crevar, Milkica | |
dc.creator | Kastritis, Panagiotis | |
dc.creator | Pletsa, Vasiliki | |
dc.creator | Nallet, Frédéric | |
dc.creator | Savić, Snežana | |
dc.creator | Xenakis, Aristotelis | |
dc.creator | Papadimitriou, Vassiliki | |
dc.date.accessioned | 2023-01-20T17:06:33Z | |
dc.date.available | 2023-01-20T17:06:33Z | |
dc.date.issued | 2023 | |
dc.identifier.issn | 0021-9797 | |
dc.identifier.uri | https://farfar.pharmacy.bg.ac.rs/handle/123456789/4384 | |
dc.description.abstract | Hypothesis: Lipophilic cannabidiol can be solubilized in oil-in water nanoemulsions, which can then be impregnated into chitosan hydrogels forming another colloidal system that will facilitate cannabidiol's release. The delivery from both systems was compared, alongside structural and biological studies, to clarify the effect of the two carriers' structure on the release and toxicity of the systems. Experiments: Oil-in-water nanoemulsions (NEs) and the respective nanoemulsion-filled chitosan hydrogels (NE/HGs) were formulated as carriers of cannabidiol (CBD). Size, polydispersity and stability of the NEs were evaluated and then membrane dynamics, shape and structure of both systems were investigated with EPR spin probing, SAXS and microscopy. Biocompatibility of the colloidal delivery systems was evaluated through cytotoxicity tests over normal human skin fibroblasts. An ex vivo permeation protocol using porcine ear skin was implemented to assess the release of CBD and its penetration through the skin. Findings: Incorporation of the NEs in chitosan hydrogels does not significantly affect their structural properties as evidenced through SAXS, EPR and confocal microscopy. These findings indicate the successful development of a novel nanocarrier that preserves the NE structure with the CBD remaining encapsulated in the oil core while providing new rheological properties advantageous over NEs. Moreover, NE/HGs proved to be more efficient as a carrier for the release of CBD. Cell viability assessment revealed high biocompatibility of the proposed colloids. | |
dc.publisher | Academic Press Inc. | |
dc.relation | The Federal Ministry of Education and Research (BMBF, ZIK program) [grant numbers 03Z22HN23 and 03Z22HI2 (to P.L.K.)] | |
dc.relation | The European Regional Development Fund for Saxony-Anhalt [grant number EFRE: ZS/2016/04/78115 (to P.L.K.)] | |
dc.relation | The Martin Luther University Halle-Wittenberg. AX thanks the University of Bordeaux | |
dc.rights | restrictedAccess | |
dc.source | Journal of Colloid and Interface Science | |
dc.subject | Cannabinoids | |
dc.subject | Cell viability | |
dc.subject | Confocal microscopy | |
dc.subject | Cryo-EM | |
dc.subject | DLS | |
dc.subject | EPR | |
dc.subject | Hydrogels | |
dc.subject | Nanoemulsions | |
dc.subject | SAXS | |
dc.subject | Skin permeation | |
dc.title | Encapsulation of cannabidiol in oil-in-water nanoemulsions and nanoemulsion-filled hydrogels: A structure and biological assessment study | |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.volume | 634 | |
dc.citation.spage | 300 | |
dc.citation.epage | 313 | |
dc.citation.rank | M21~ | |
dc.identifier.wos | 000907036200001 | |
dc.identifier.doi | 10.1016/j.jcis.2022.12.036 | |
dc.identifier.pmid | 36535166 | |
dc.identifier.scopus | 2-s2.0-85144443807 | |
dc.type.version | publishedVersion |