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dc.creatorDokmanović, Jelena
dc.creatorKasagić-Vujanović, Irena
dc.creatorGagić, Žarko
dc.creatorNikolić, Katarina
dc.creatorČarapić, Marija
dc.creatorAgbaba, Danica
dc.date.accessioned2023-03-07T10:38:53Z
dc.date.available2023-03-07T10:38:53Z
dc.date.issued2023
dc.identifier.issn1233-2356
dc.identifier.urihttps://farfar.pharmacy.bg.ac.rs/handle/123456789/4474
dc.description.abstractUsing the Design of Experiments methodology (Response-Surface Methodology and Derringer's Desirability Function), a simple, fast and robust RP-HPLC method was developed for the analysis of enrofloxacin (EFC), its impurity A (fluoroquinolonic acid, FQ) and impurity B (ciprofloxacin, CPX). Gradient elution of samples was performed on a Zorbax Eclipse XDB C18 column (150 × 4.6 mm, 3.5 μm) with a mobile phase consisting of 32 mM phosphate buffer pH 3.5-methanol (0 min-19.6% methanol; 15.5 min-19.6% methanol; 29.5 min-80% methanol; 30 min-19.6% methanol; 35 min-19.6% methanol), delivered at a flow rate of 1.5 mL min-1, wavelength of detection 278 nm (for EFX and CFX) and 265 nm for FQ. A good linear response was achieved in the range 15-35 μg mL-1 (EFX) and LOQ-150% for impurities (CFX and FQ). Other validation parameters were also tested: precision, accuracy, sensitivity and robustness. The developed method was shown to be simple, practical and suitable for the analysis of EFC and its impurities (CPX, FQ) in veterinary drugs.
dc.publisherAkademiai Kiado ZRt.
dc.relationThe Ministry for Scientific and Technolog- ical Development, Higher Education and Information Society of the Republic of Srpska for supporting these investigations within the framework of the Project 19.032/961-149/19
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.sourceActa Chromatographica
dc.subjectRP-HPLC
dc.subjectDerringer's desirability function
dc.subjectdesign of experiments
dc.subjectenrofloxacin
dc.subjectoptimisation method
dc.subjectresponse surface methodology
dc.titleDesign of experiments and Derringer's desirability function in optimisation and validation of RP-HPLC method for the analysis of enrofloxacin and its impurities
dc.typearticle
dc.rights.licenseBY-NC
dc.citation.rankM23~
dc.identifier.doi10.1556/1326.2022.01111
dc.identifier.scopus2-s2.0-85148616086
dc.identifier.fulltexthttp://farfar.pharmacy.bg.ac.rs/bitstream/id/12035/Design_of_experiments_pub_2023.pdf
dc.type.versionpublishedVersion


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Приказ основних података о документу