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Optimization of the RP-HPLC method for multicomponent analgetic drug determination.

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Authors
Ivanović, D
Medenica, Mirjana
Malenović, Anđelija
Jančić, Biljana
Misljenović, D
Article (Published version)
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Abstract
The optimization of RP-HPLC method defined the simultaneous influence of some important conditions, such as the mobile phase composition, pH of the mobile phase and temperature, on the separation and determination. The RP-HPLC method was done for the determination of paracetamol, caffeine and propyphenazone in a multicomponent pharmaceutical dosage form. The separation factor values define the optimal conditions, which were confirmed by analysing the appropriate mathematical models. The chromatographic system Hewlett Packard 1100 consisted of a HP 1100 pump, HP 1100 UV-VIS detector and HP integrator. Separations were performed on a Beckman Ultrasphere ODS 4.6 x 150 mm, 5 microns particle size column. Samples were introduced through a Rheodyne injector valve with a 20 microL sample loop. UV detection was performed at 265 nm and phenobarottons was used as an internal standard. The optimization was performed within the pH range from 2.5 to 6.0; temperature range from 20 degrees C to 55 de...grees C and composition of the mobile phase methanol-water from (30:70 V/V) to (65:35 V/V). The three-D graphs, constructed with sixty-four experimental points, confirmed the optimal conditions for the determination of the investigated analgetic drugs.

Source:
Bollettino chimico farmaceutico, 2003, 142, 9, 386-389

ISSN: 0006-6648

Scopus: 2-s2.0-1542715918
[ Google Scholar ]
7
Handle
https://hdl.handle.net/21.15107/rcub_farfar_460
URI
https://farfar.pharmacy.bg.ac.rs/handle/123456789/460
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Pharmacy
TY  - JOUR
AU  - Ivanović, D
AU  - Medenica, Mirjana
AU  - Malenović, Anđelija
AU  - Jančić, Biljana
AU  - Misljenović, D
PY  - 2003
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/460
AB  - The optimization of RP-HPLC method defined the simultaneous influence of some important conditions, such as the mobile phase composition, pH of the mobile phase and temperature, on the separation and determination. The RP-HPLC method was done for the determination of paracetamol, caffeine and propyphenazone in a multicomponent pharmaceutical dosage form. The separation factor values define the optimal conditions, which were confirmed by analysing the appropriate mathematical models. The chromatographic system Hewlett Packard 1100 consisted of a HP 1100 pump, HP 1100 UV-VIS detector and HP integrator. Separations were performed on a Beckman Ultrasphere ODS 4.6 x 150 mm, 5 microns particle size column. Samples were introduced through a Rheodyne injector valve with a 20 microL sample loop. UV detection was performed at 265 nm and phenobarottons was used as an internal standard. The optimization was performed within the pH range from 2.5 to 6.0; temperature range from 20 degrees C to 55 degrees C and composition of the mobile phase methanol-water from (30:70 V/V) to (65:35 V/V). The three-D graphs, constructed with sixty-four experimental points, confirmed the optimal conditions for the determination of the investigated analgetic drugs.
T2  - Bollettino chimico farmaceutico
T1  - Optimization of the RP-HPLC method for multicomponent analgetic drug determination.
VL  - 142
IS  - 9
SP  - 386
EP  - 389
UR  - https://hdl.handle.net/21.15107/rcub_farfar_460
ER  - 
@article{
author = "Ivanović, D and Medenica, Mirjana and Malenović, Anđelija and Jančić, Biljana and Misljenović, D",
year = "2003",
abstract = "The optimization of RP-HPLC method defined the simultaneous influence of some important conditions, such as the mobile phase composition, pH of the mobile phase and temperature, on the separation and determination. The RP-HPLC method was done for the determination of paracetamol, caffeine and propyphenazone in a multicomponent pharmaceutical dosage form. The separation factor values define the optimal conditions, which were confirmed by analysing the appropriate mathematical models. The chromatographic system Hewlett Packard 1100 consisted of a HP 1100 pump, HP 1100 UV-VIS detector and HP integrator. Separations were performed on a Beckman Ultrasphere ODS 4.6 x 150 mm, 5 microns particle size column. Samples were introduced through a Rheodyne injector valve with a 20 microL sample loop. UV detection was performed at 265 nm and phenobarottons was used as an internal standard. The optimization was performed within the pH range from 2.5 to 6.0; temperature range from 20 degrees C to 55 degrees C and composition of the mobile phase methanol-water from (30:70 V/V) to (65:35 V/V). The three-D graphs, constructed with sixty-four experimental points, confirmed the optimal conditions for the determination of the investigated analgetic drugs.",
journal = "Bollettino chimico farmaceutico",
title = "Optimization of the RP-HPLC method for multicomponent analgetic drug determination.",
volume = "142",
number = "9",
pages = "386-389",
url = "https://hdl.handle.net/21.15107/rcub_farfar_460"
}
Ivanović, D., Medenica, M., Malenović, A., Jančić, B.,& Misljenović, D.. (2003). Optimization of the RP-HPLC method for multicomponent analgetic drug determination.. in Bollettino chimico farmaceutico, 142(9), 386-389.
https://hdl.handle.net/21.15107/rcub_farfar_460
Ivanović D, Medenica M, Malenović A, Jančić B, Misljenović D. Optimization of the RP-HPLC method for multicomponent analgetic drug determination.. in Bollettino chimico farmaceutico. 2003;142(9):386-389.
https://hdl.handle.net/21.15107/rcub_farfar_460 .
Ivanović, D, Medenica, Mirjana, Malenović, Anđelija, Jančić, Biljana, Misljenović, D, "Optimization of the RP-HPLC method for multicomponent analgetic drug determination." in Bollettino chimico farmaceutico, 142, no. 9 (2003):386-389,
https://hdl.handle.net/21.15107/rcub_farfar_460 .

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