Anti-alpha amylase activity of Macrolepiota procera (Scop.: Fr.) Sing.

Abstract

The parasol mushroom Macrolepiota procera (Scop.:Fr.) Sing, Lepiotaceae, is a highly valued edible type of fungi. Its top is an egg-shaped, gray to brown hat, covered in peal-like marks. This mushroom resides on a layer of decomposing leaves in all types of the forests, appearing solitary or in small groups from late summer to autumn. Besides good taste, it has good nutritional and medicinal value. So far, scientific studies on M. procera have shown its antihypertensive, antioxidative, antiproliferative and cytotoxic abilities. Inhibitory effect against alpha-amylase, total polyphenolic content and antiradical scavenging activity of methanol extracts prepared from dry mushroom from local market in Belgrade was the main goal of this study. Alpha-amylase inhibitory assay was assessed spectrophotometrically with 3,5-dinitrosalicylic acid reagent. Total polyphenolic content and antiradical activity in methanolic extract was determined using common Folin-Ciocalteu method and DPPH assay. Concentration of methanolic extract that inhibited 50% alpha-amylase (IC50) was 0.66 mg/mL. The content of polyphenols in methanolic extract was 13.07±0.24 mg of gallic acid/g dry extract. The concentration that neutralizes 50% DPPH radicals was 466.45±5.52 μg/mL, which was weaker antiradical activity than standard antioxidants vitamin C (IC50 3.8 μg/ mL) and quercetin (IC50 2.75 μg/mL).