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The effects of acth, isoproterenol and Dexamethasone on the rat adrenal gland response to ethane dimethanesulphonate (Eds): A stereological study

Uticaj ACTH, izoproterenola i deksametazona na odgovor nadbubrežne žlezde pacova na etandimetansulfonat (EDS): stereološko ispitivanje

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2003
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Authors
Plećaš-Solarović, Bosiljka
Pešić, Vesna
Mirković, Duško
Leposavić, Gordana
Article (Published version)
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Abstract
Ethane dimethanesulphonate (EDS), an alkylating agent, caused marked atrophy of the adrenal cortex of adult male rats, in addition to its toxic effect on testicular Leydig cells. The aim of this work was to examine whether a 9-day treatment with ACTH (40 IU/kg/d), isoproterenol (120 mg/kg/d) or dexamethasone (0.25 mg/kg/d), which started 4 days prior to intraperitoneal administration of a single dose of EDS (75 mg/kg), affected the morphological changes in the adrenal cortex evoked by EDS alone. The animals were killed 15 days after EDS injection. Stereological analysis revealed that both ACTH and isoproterenol almost completely prevented cortical atrophy induced by EDS. They also considerably stimulated corticosterone secretion in EDS-injected animals. By contrast, in dexamethasone-suppressed rats, the deleterious effect of EDS on adrenocortical cells was augmented. The volume and cellularity of all cortical zones were reduced, but the remaining cells of the zona reticularis displayed... considerable hypertrophy which was probably responsible for the maintenance of corticosterone secretion. These results clearly demonstrate that both ACTH and b adrenoceptor stimulation have protective action against the toxic effects of EDS on rat adrenal cortex, whereas dexamethasone exerts an opposite influence.

Alkilirajući agens etandimetansulfonat (EDS), pored toksičnog delovanja na Lajdigove ćelije semenika, izaziva izraženu atrofiju kore nadbubrežne žlezde odraslih pacova. U ovom radu je ispitivan uticaj devetodnevnog tretmana sa ACTH (40 IU/kg/d), izoproterenolom (120 mg/kg/d) ili deksametazonom (0.25 mg/kg/d) na morfološke promene kore nadbubrega koje izaziva jedna intraperitonealna injekcija EDS (75 mg/kg). Tretmani su započeli 4 dana pre davanja EDS i nastavljeni su još 5 dana, a životinje su žrtvovane 15 dana posle aplikacije EDS. U prisustvu ACTH ili izoproterenola ne ispoljavaju se promene u stereološkim parametrima kore nadbubrega koje izaziva EDS. Oba tretmana značajno povećavaju i koncentraciju kortikosterona u serumu. Deksametazon, međutim, pojačava toksične efekte EDS; zapremina svih zona kore i broj parenhimskih ćelija u zonama su smanjeni, ali preostale ćelije retikularne zone pokazuju značajnu hipertrofiju, koja je verovatno odgovorna za održavanje sekrecije kortikosterona ...kod ovih životinja. Rezultati rada pokazuju da se toksični efekti EDS na koru nadbubrega mogu sprečiti visokim nivoom ACTH ili stimulacijom b adrenalinskih receptora, a potencirati egzogenim glukokortikoidom.

Keywords:
Ethane dimethanesulphonate / rat adrenal cortex / ACTH / isoproterenol / dexamethasone / stereology / corticosterone
Source:
Acta veterinaria, 2003, 53, 5-6, 333-342
Publisher:
  • Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd

DOI: 10.2298/AVB0306333P

ISSN: 0567-8315

WoS: 000187620300005

Scopus: 2-s2.0-1642534315
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1
1
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https://farfar.pharmacy.bg.ac.rs/handle/123456789/468
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  • Radovi istraživača / Researchers’ publications
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Pharmacy
TY  - JOUR
AU  - Plećaš-Solarović, Bosiljka
AU  - Pešić, Vesna
AU  - Mirković, Duško
AU  - Leposavić, Gordana
PY  - 2003
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/468
AB  - Ethane dimethanesulphonate (EDS), an alkylating agent, caused marked atrophy of the adrenal cortex of adult male rats, in addition to its toxic effect on testicular Leydig cells. The aim of this work was to examine whether a 9-day treatment with ACTH (40 IU/kg/d), isoproterenol (120 mg/kg/d) or dexamethasone (0.25 mg/kg/d), which started 4 days prior to intraperitoneal administration of a single dose of EDS (75 mg/kg), affected the morphological changes in the adrenal cortex evoked by EDS alone. The animals were killed 15 days after EDS injection. Stereological analysis revealed that both ACTH and isoproterenol almost completely prevented cortical atrophy induced by EDS. They also considerably stimulated corticosterone secretion in EDS-injected animals. By contrast, in dexamethasone-suppressed rats, the deleterious effect of EDS on adrenocortical cells was augmented. The volume and cellularity of all cortical zones were reduced, but the remaining cells of the zona reticularis displayed considerable hypertrophy which was probably responsible for the maintenance of corticosterone secretion. These results clearly demonstrate that both ACTH and b adrenoceptor stimulation have protective action against the toxic effects of EDS on rat adrenal cortex, whereas dexamethasone exerts an opposite influence.
AB  - Alkilirajući agens etandimetansulfonat (EDS), pored toksičnog delovanja na Lajdigove ćelije semenika, izaziva izraženu atrofiju kore nadbubrežne žlezde odraslih pacova. U ovom radu je ispitivan uticaj devetodnevnog tretmana sa ACTH (40 IU/kg/d), izoproterenolom (120 mg/kg/d) ili deksametazonom (0.25 mg/kg/d) na morfološke promene kore nadbubrega koje izaziva jedna intraperitonealna injekcija EDS (75 mg/kg). Tretmani su započeli 4 dana pre davanja EDS i nastavljeni su još 5 dana, a životinje su žrtvovane 15 dana posle aplikacije EDS. U prisustvu ACTH ili izoproterenola ne ispoljavaju se promene u stereološkim parametrima kore nadbubrega koje izaziva EDS. Oba tretmana značajno povećavaju i koncentraciju kortikosterona u serumu. Deksametazon, međutim, pojačava toksične efekte EDS; zapremina svih zona kore i broj parenhimskih ćelija u zonama su smanjeni, ali preostale ćelije retikularne zone pokazuju značajnu hipertrofiju, koja je verovatno odgovorna za održavanje sekrecije kortikosterona kod ovih životinja. Rezultati rada pokazuju da se toksični efekti EDS na koru nadbubrega mogu sprečiti visokim nivoom ACTH ili stimulacijom b adrenalinskih receptora, a potencirati egzogenim glukokortikoidom.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria
T1  - The effects of acth, isoproterenol and Dexamethasone on the rat adrenal gland response to ethane dimethanesulphonate (Eds): A stereological study
T1  - Uticaj ACTH, izoproterenola i deksametazona na odgovor nadbubrežne žlezde pacova na etandimetansulfonat (EDS): stereološko ispitivanje
VL  - 53
IS  - 5-6
SP  - 333
EP  - 342
DO  - 10.2298/AVB0306333P
ER  - 
@article{
author = "Plećaš-Solarović, Bosiljka and Pešić, Vesna and Mirković, Duško and Leposavić, Gordana",
year = "2003",
abstract = "Ethane dimethanesulphonate (EDS), an alkylating agent, caused marked atrophy of the adrenal cortex of adult male rats, in addition to its toxic effect on testicular Leydig cells. The aim of this work was to examine whether a 9-day treatment with ACTH (40 IU/kg/d), isoproterenol (120 mg/kg/d) or dexamethasone (0.25 mg/kg/d), which started 4 days prior to intraperitoneal administration of a single dose of EDS (75 mg/kg), affected the morphological changes in the adrenal cortex evoked by EDS alone. The animals were killed 15 days after EDS injection. Stereological analysis revealed that both ACTH and isoproterenol almost completely prevented cortical atrophy induced by EDS. They also considerably stimulated corticosterone secretion in EDS-injected animals. By contrast, in dexamethasone-suppressed rats, the deleterious effect of EDS on adrenocortical cells was augmented. The volume and cellularity of all cortical zones were reduced, but the remaining cells of the zona reticularis displayed considerable hypertrophy which was probably responsible for the maintenance of corticosterone secretion. These results clearly demonstrate that both ACTH and b adrenoceptor stimulation have protective action against the toxic effects of EDS on rat adrenal cortex, whereas dexamethasone exerts an opposite influence., Alkilirajući agens etandimetansulfonat (EDS), pored toksičnog delovanja na Lajdigove ćelije semenika, izaziva izraženu atrofiju kore nadbubrežne žlezde odraslih pacova. U ovom radu je ispitivan uticaj devetodnevnog tretmana sa ACTH (40 IU/kg/d), izoproterenolom (120 mg/kg/d) ili deksametazonom (0.25 mg/kg/d) na morfološke promene kore nadbubrega koje izaziva jedna intraperitonealna injekcija EDS (75 mg/kg). Tretmani su započeli 4 dana pre davanja EDS i nastavljeni su još 5 dana, a životinje su žrtvovane 15 dana posle aplikacije EDS. U prisustvu ACTH ili izoproterenola ne ispoljavaju se promene u stereološkim parametrima kore nadbubrega koje izaziva EDS. Oba tretmana značajno povećavaju i koncentraciju kortikosterona u serumu. Deksametazon, međutim, pojačava toksične efekte EDS; zapremina svih zona kore i broj parenhimskih ćelija u zonama su smanjeni, ali preostale ćelije retikularne zone pokazuju značajnu hipertrofiju, koja je verovatno odgovorna za održavanje sekrecije kortikosterona kod ovih životinja. Rezultati rada pokazuju da se toksični efekti EDS na koru nadbubrega mogu sprečiti visokim nivoom ACTH ili stimulacijom b adrenalinskih receptora, a potencirati egzogenim glukokortikoidom.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria",
title = "The effects of acth, isoproterenol and Dexamethasone on the rat adrenal gland response to ethane dimethanesulphonate (Eds): A stereological study, Uticaj ACTH, izoproterenola i deksametazona na odgovor nadbubrežne žlezde pacova na etandimetansulfonat (EDS): stereološko ispitivanje",
volume = "53",
number = "5-6",
pages = "333-342",
doi = "10.2298/AVB0306333P"
}
Plećaš-Solarović, B., Pešić, V., Mirković, D.,& Leposavić, G.. (2003). The effects of acth, isoproterenol and Dexamethasone on the rat adrenal gland response to ethane dimethanesulphonate (Eds): A stereological study. in Acta veterinaria
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 53(5-6), 333-342.
https://doi.org/10.2298/AVB0306333P
Plećaš-Solarović B, Pešić V, Mirković D, Leposavić G. The effects of acth, isoproterenol and Dexamethasone on the rat adrenal gland response to ethane dimethanesulphonate (Eds): A stereological study. in Acta veterinaria. 2003;53(5-6):333-342.
doi:10.2298/AVB0306333P .
Plećaš-Solarović, Bosiljka, Pešić, Vesna, Mirković, Duško, Leposavić, Gordana, "The effects of acth, isoproterenol and Dexamethasone on the rat adrenal gland response to ethane dimethanesulphonate (Eds): A stereological study" in Acta veterinaria, 53, no. 5-6 (2003):333-342,
https://doi.org/10.2298/AVB0306333P . .

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