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Određivanje sub-mikrograma hesperidina metodom perturbacije Bray -Liebhafsky oscilatorne reakcije realizovane u otvorenom reaktoru

dc.creatorPejić, Nataša
dc.creatorBlagojević, Slavica
dc.date.accessioned2019-09-02T10:57:56Z
dc.date.available2019-09-02T10:57:56Z
dc.date.issued2003
dc.identifier.issn0353-5517
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/472
dc.description.abstractResults obtained by kinetic method based on hesperidin interaction with Bray-Liebhafsky oscillatory reaction, as the matrix established in non-equilibrium stationary state in the vicinity of bifurcation point, are given. In these conditions, the matrix system is extremely sensitive on changes of parameter so that pulse perturbations performed by addition of micro amount of hesperidin were caused changes in system whish is followed potentiometricaly by Pt electrode. This kinetic procedure established on linear relationship between logarithm concentration of hesperidin and potential shift (defined as the difference in potential after the perturbation and potential of stationary state) that was obtained in range concentrations of hesperidin from 6.8×10-3 to 5.4×10-1 μg mL-1. Obtained limit of detection is 1.7×10-3 μg mL-1. Routine is successfully concerned for quantitative determination of hesperidin in real sample (capsules). The recovery determination of hesperidin from capsules was found to be 97.3 %. Thus, for the expected 109.8 mg Hesp, 107.4 mg were recovered by the applied method (RSD = 2.3%). Developed kinetic method is simple, sensitive and accurate.en
dc.description.abstractPrikazani su rezultati analize dobijeni kinetičkom metodom zasnovanom na interakciji hesperidina i Bray-Liebhafsky oscilatorne reakcije kao matrice kada se ista nalazi u neravnotežnom stabilnom stacionarnom stanju u blizini bifurkacione tačke. U ovim uslovima matrični sistem ispoljava izuzetnu osetljivost na promene parametara tako da pulsne perturbacije izazvane dodatkom mikrokoličina hesperidina dovode do promena u sistemu koje mogu da se prate potenciometrijski korišćenjem Pt elektrode. Ovaj kinetički postupak zasniva se na linearnoj vezi između logaritma koncentracije hesperidina i promene potencijala (definisanog kao razlika u potencijalu nakon izvršene perturbacije i potencijala stacionarnog stanja) koja je dobijena za koncentracije hesperidina u opsegu 6,8×10-3- 5,4×10-1 μg mL-1. Dobijeni limit detekcije iznosi 1,7×10-3 μg mL-1. Postupak je uspešno primenjen za kvantitativno određivanje hesperidina u realnom uzorku (kapsule). Tačnost metode (data recovery vrednostima) iznosi 97,3 %. Tako, od očekivanog sadržaja hesperidina u kapsuli od 109,8 mg, nađeno je 107,4 mg (RSD = 2,3%). Razvijena kinetička metoda je jednostavna, osetljiva i pouzdana.sr
dc.publisherInstitut bezbednosti, Beograd
dc.rightsopenAccess
dc.sourceNauka, tehnika, bezbednost
dc.subjectBray-Liebhafsky oscillatory reactionen
dc.subjectflavonoiden
dc.subjecthesperidinen
dc.subjectperturbation techniqueen
dc.subjectpotentiometricen
dc.subjectBray-Liebhafsky oscilatorna reakcijasr
dc.subjectflavonoidisr
dc.subjecthesperidinsr
dc.subjectperturbaciona tehnikasr
dc.subjectpotenciometrijasr
dc.titleDetermination of sub-micrograms of hesperidin by method of perturbation of Bray-Liebhafsky oscillatory reaction realized in an open reactoren
dc.titleOdređivanje sub-mikrograma hesperidina metodom perturbacije Bray -Liebhafsky oscilatorne reakcije realizovane u otvorenom reaktorusr
dc.typearticle
dc.rights.licenseARR
dcterms.abstractПејић, Наташа; Благојевић, Славица;
dc.citation.volume13
dc.citation.issue2
dc.citation.spage81
dc.citation.epage88
dc.citation.other13(2): 81-88
dc.identifier.rcubconv_809
dc.type.versionpublishedVersion


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