Comparison of HPTLC and HPLC for determination of econazole nitrate in topical dosage forms
Abstract
HPTLC and HPLC methods have been established for separation and quantitative determination of econazole nitrate. HPTLC was performed on silica gel plates with n-butyl acetate-carbon tetrachloride-methanol-diethylamine, 3 + 6 + 2.5 + 0.5 (v/v), as mobile phase. Chromatographic zones, or spots, of econazole base and nitrate were used to quantify econazole nitrate. HPLC was performed on amino and C-8 columns with acetonitrile-water, 30 + 70 (v/v), pH 2.5 (adjusted with phosphoric acid), as a mobile phase. The chromatograms were characterized by single peaks of econazole (amino column) or nitrate (C-8 column). The methods were successfully used for determination of econazole nitrate in spray solution and vaginal pessaries.
Keywords:
econazole nitrate / HPTLC / HPLC / pharmaceutical formulationsSource:
Journal of Planar Chromatography - Modern TLC, 2004, 17, 2, 109-112Publisher:
- Research Inst Medicinal Plants, Budakalasz
DOI: 10.1556/JPC.17.2004.2.5
ISSN: 0933-4173
WoS: 000221981300005
Scopus: 2-s2.0-2942558545
Collections
Institution/Community
PharmacyTY - JOUR AU - Popović, Gordana AU - Čakar, Mira AU - Vučićević, Katarina AU - Vladimirov, S AU - Agbaba, Danica PY - 2004 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/479 AB - HPTLC and HPLC methods have been established for separation and quantitative determination of econazole nitrate. HPTLC was performed on silica gel plates with n-butyl acetate-carbon tetrachloride-methanol-diethylamine, 3 + 6 + 2.5 + 0.5 (v/v), as mobile phase. Chromatographic zones, or spots, of econazole base and nitrate were used to quantify econazole nitrate. HPLC was performed on amino and C-8 columns with acetonitrile-water, 30 + 70 (v/v), pH 2.5 (adjusted with phosphoric acid), as a mobile phase. The chromatograms were characterized by single peaks of econazole (amino column) or nitrate (C-8 column). The methods were successfully used for determination of econazole nitrate in spray solution and vaginal pessaries. PB - Research Inst Medicinal Plants, Budakalasz T2 - Journal of Planar Chromatography - Modern TLC T1 - Comparison of HPTLC and HPLC for determination of econazole nitrate in topical dosage forms VL - 17 IS - 2 SP - 109 EP - 112 DO - 10.1556/JPC.17.2004.2.5 ER -
@article{ author = "Popović, Gordana and Čakar, Mira and Vučićević, Katarina and Vladimirov, S and Agbaba, Danica", year = "2004", abstract = "HPTLC and HPLC methods have been established for separation and quantitative determination of econazole nitrate. HPTLC was performed on silica gel plates with n-butyl acetate-carbon tetrachloride-methanol-diethylamine, 3 + 6 + 2.5 + 0.5 (v/v), as mobile phase. Chromatographic zones, or spots, of econazole base and nitrate were used to quantify econazole nitrate. HPLC was performed on amino and C-8 columns with acetonitrile-water, 30 + 70 (v/v), pH 2.5 (adjusted with phosphoric acid), as a mobile phase. The chromatograms were characterized by single peaks of econazole (amino column) or nitrate (C-8 column). The methods were successfully used for determination of econazole nitrate in spray solution and vaginal pessaries.", publisher = "Research Inst Medicinal Plants, Budakalasz", journal = "Journal of Planar Chromatography - Modern TLC", title = "Comparison of HPTLC and HPLC for determination of econazole nitrate in topical dosage forms", volume = "17", number = "2", pages = "109-112", doi = "10.1556/JPC.17.2004.2.5" }
Popović, G., Čakar, M., Vučićević, K., Vladimirov, S.,& Agbaba, D.. (2004). Comparison of HPTLC and HPLC for determination of econazole nitrate in topical dosage forms. in Journal of Planar Chromatography - Modern TLC Research Inst Medicinal Plants, Budakalasz., 17(2), 109-112. https://doi.org/10.1556/JPC.17.2004.2.5
Popović G, Čakar M, Vučićević K, Vladimirov S, Agbaba D. Comparison of HPTLC and HPLC for determination of econazole nitrate in topical dosage forms. in Journal of Planar Chromatography - Modern TLC. 2004;17(2):109-112. doi:10.1556/JPC.17.2004.2.5 .
Popović, Gordana, Čakar, Mira, Vučićević, Katarina, Vladimirov, S, Agbaba, Danica, "Comparison of HPTLC and HPLC for determination of econazole nitrate in topical dosage forms" in Journal of Planar Chromatography - Modern TLC, 17, no. 2 (2004):109-112, https://doi.org/10.1556/JPC.17.2004.2.5 . .