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dc.creatorHomšek, Ana
dc.creatorMarković, Bojan
dc.creatorBogavac-Stanojević, Nataša
dc.creatorVladimirov, Sote
dc.creatorKarljiković-Rajić, Katarina
dc.date.accessioned2023-06-22T07:38:47Z
dc.date.available2023-06-22T07:38:47Z
dc.date.issued2020
dc.identifier.issn0003-7028
dc.identifier.urihttps://farfar.pharmacy.bg.ac.rs/handle/123456789/4833
dc.description.abstractThe application assessment of different programs was performed with equivalence tests for method transfer pro second-order derivative spectrophotometry. The digital second-order derivative spectra were calculated on different instruments; GBC Scientific Equipment Cintra 20 (Cintral v.2.6 and Spectral v.1.70 software programs) and Thermo Scientific Evolution 300 (VISIONPro software) were analyzed using the amplitude A/B ratio (A = 2D265,263; B = 2D263,261). Amplitude A/B ratio is the resolution parameter for derivative spectrophotometry prescribed in European Pharmacopoeia. The obtained values for A/B ratio were either very similar or significantly different among programs: 0.669 (Cintral v.2.6), 0.549 (Spectral v.1.70), 0.556 (medium indirect VISIONPro), 0.557 (one-step Savitzky–Golay 7 VISIONPro), 0.689 (two-step Savitzky–Golay 7 VISIONPro). Method transfer was possible between Spectral v.1.70 and VISIONPro (medium indirect and one-step Savitzky–Golay 7), but the values obtained in Cintral v.2.6 were not comparable to the other programs. The absorbance data exported from both instruments were additionally calculated in OriginPro8 which provided almost the same mean A/B values (0.627 Cintral v.2.6; 0.624 VISIONPro), confirming that the two instruments recorded the same zero-order spectra. The calculation of resolution parameter could be used for verification of program comparison, which would enable transfer between sender and receiver laboratory. The accordance between program algorithms was confirmed when acceptable differences for values of resolution parameter (A/B ratios) were achieved.sr
dc.language.isoensr
dc.publisherSAGE Publicationssr
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172041/RS//sr
dc.rightsrestrictedAccesssr
dc.sourceApplied Spectroscopysr
dc.subjectDigital derivative spectrasr
dc.subjectEPsr
dc.subjectEuropean Pharmacopoeiasr
dc.subjectamplitude A/B ratio–resolution parametersr
dc.subjectequivalence testssr
dc.subjectmethod transfersr
dc.subjectprogram algorithms evaluationsr
dc.titleMethod Transfer Evaluation for Digital Derivative Spectrophotometry Through its Resolution Parameter Comparison of Different Computer Programssr
dc.typearticlesr
dc.rights.licenseARRsr
dc.citation.volume74
dc.citation.issue5
dc.citation.spage525
dc.citation.epage535
dc.citation.rankM22
dc.identifier.wos000523834400001
dc.identifier.doi10.1177/0003702819889374
dc.identifier.pmid32167375
dc.identifier.scopus2-s2.0-85082715464
dc.type.versionpublishedVersionsr


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