HDL Subclasses and the Distribution of Paraoxonase-1 Activity in Patients with ST-Segment Elevation Acute Myocardial Infarction
Authors
Đekić, SaškaVekić, Jelena

Zeljković, Aleksandra

Kotur-Stevuljević, Jelena

Kafedžić, Srđan
Zdravković, Marija
Ilić, Ivan
Hinić, Saša
Cerović, Milivoje
Stefanović, Milica
Mihajlović, Marija

Nešković, Aleksandar
Bogavac-Stanojević, Nataša

Article (Published version)
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Show full item recordAbstract
The aim of this multicentric study was to assess the impacts of oxidative stress, inflam-
mation, and the presence of small, dense, low-density lipoproteins (sdLDL) on the antioxidative
function of high-density lipoprotein (HDL) subclasses and the distribution of paraoxonase-1 (PON1)
activity within HDL in patients with ST-segment elevation acute myocardial infarction (STEMI). In
69 STEMI patients and 67 healthy control subjects, the lipoproteins’ subclasses were separated using
polyacrylamide gradient (3–31%) gel electrophoresis. The relative proportion of sdLDL and each
HDL subclass was evaluated by measuring the areas under the peaks of densitometric scans. The
distribution of the relative proportion of PON1 activity within the HDL subclasses (pPON1 within
HDL) was estimated using the zymogram method. The STEMI patients had significantly lower
proportions of HDL2a and HDL3a subclasses (p = 0.001 and p < 0.001, respectively) and lower pPON1
within HDL3b (p = 0.006), as well... as higher proportions of HDL3b and HDL3c subclasses (p = 0.013
and p < 0.001, respectively) and higher pPON1 within HDL2 than the controls. Independent positive
associations between sdLDL and pPON1 within HDL3a and between malondialdehyde (MDA) and
pPON1 within HDL2b were shown in the STEMI group. The increased oxidative stress and increased
proportion of sdLDL in STEMI are closely related to the compromised antioxidative function of small
HDL3 particles and the altered pPON1 within HDL.
Keywords:
high-density lipoprotein (HDL) subclasses / paraoxonase-1 (PON1) / small / dense / low-density lipoprotein (sdLDL)Source:
International Journal of Molecular Sciences, 2023, 24, 11Publisher:
- MDPI
Funding / projects:
DOI: 10.3390/ijms24119384
ISSN: 1661-6596
PubMed: 37298334
WoS: 001003450900001
Scopus: 2-s2.0-85161925404
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Institution/Community
PharmacyTY - JOUR AU - Đekić, Saška AU - Vekić, Jelena AU - Zeljković, Aleksandra AU - Kotur-Stevuljević, Jelena AU - Kafedžić, Srđan AU - Zdravković, Marija AU - Ilić, Ivan AU - Hinić, Saša AU - Cerović, Milivoje AU - Stefanović, Milica AU - Mihajlović, Marija AU - Nešković, Aleksandar AU - Bogavac-Stanojević, Nataša PY - 2023 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4843 AB - The aim of this multicentric study was to assess the impacts of oxidative stress, inflam- mation, and the presence of small, dense, low-density lipoproteins (sdLDL) on the antioxidative function of high-density lipoprotein (HDL) subclasses and the distribution of paraoxonase-1 (PON1) activity within HDL in patients with ST-segment elevation acute myocardial infarction (STEMI). In 69 STEMI patients and 67 healthy control subjects, the lipoproteins’ subclasses were separated using polyacrylamide gradient (3–31%) gel electrophoresis. The relative proportion of sdLDL and each HDL subclass was evaluated by measuring the areas under the peaks of densitometric scans. The distribution of the relative proportion of PON1 activity within the HDL subclasses (pPON1 within HDL) was estimated using the zymogram method. The STEMI patients had significantly lower proportions of HDL2a and HDL3a subclasses (p = 0.001 and p < 0.001, respectively) and lower pPON1 within HDL3b (p = 0.006), as well as higher proportions of HDL3b and HDL3c subclasses (p = 0.013 and p < 0.001, respectively) and higher pPON1 within HDL2 than the controls. Independent positive associations between sdLDL and pPON1 within HDL3a and between malondialdehyde (MDA) and pPON1 within HDL2b were shown in the STEMI group. The increased oxidative stress and increased proportion of sdLDL in STEMI are closely related to the compromised antioxidative function of small HDL3 particles and the altered pPON1 within HDL. PB - MDPI T2 - International Journal of Molecular Sciences T1 - HDL Subclasses and the Distribution of Paraoxonase-1 Activity in Patients with ST-Segment Elevation Acute Myocardial Infarction VL - 24 IS - 11 DO - 10.3390/ijms24119384 ER -
@article{ author = "Đekić, Saška and Vekić, Jelena and Zeljković, Aleksandra and Kotur-Stevuljević, Jelena and Kafedžić, Srđan and Zdravković, Marija and Ilić, Ivan and Hinić, Saša and Cerović, Milivoje and Stefanović, Milica and Mihajlović, Marija and Nešković, Aleksandar and Bogavac-Stanojević, Nataša", year = "2023", abstract = "The aim of this multicentric study was to assess the impacts of oxidative stress, inflam- mation, and the presence of small, dense, low-density lipoproteins (sdLDL) on the antioxidative function of high-density lipoprotein (HDL) subclasses and the distribution of paraoxonase-1 (PON1) activity within HDL in patients with ST-segment elevation acute myocardial infarction (STEMI). In 69 STEMI patients and 67 healthy control subjects, the lipoproteins’ subclasses were separated using polyacrylamide gradient (3–31%) gel electrophoresis. The relative proportion of sdLDL and each HDL subclass was evaluated by measuring the areas under the peaks of densitometric scans. The distribution of the relative proportion of PON1 activity within the HDL subclasses (pPON1 within HDL) was estimated using the zymogram method. The STEMI patients had significantly lower proportions of HDL2a and HDL3a subclasses (p = 0.001 and p < 0.001, respectively) and lower pPON1 within HDL3b (p = 0.006), as well as higher proportions of HDL3b and HDL3c subclasses (p = 0.013 and p < 0.001, respectively) and higher pPON1 within HDL2 than the controls. Independent positive associations between sdLDL and pPON1 within HDL3a and between malondialdehyde (MDA) and pPON1 within HDL2b were shown in the STEMI group. The increased oxidative stress and increased proportion of sdLDL in STEMI are closely related to the compromised antioxidative function of small HDL3 particles and the altered pPON1 within HDL.", publisher = "MDPI", journal = "International Journal of Molecular Sciences", title = "HDL Subclasses and the Distribution of Paraoxonase-1 Activity in Patients with ST-Segment Elevation Acute Myocardial Infarction", volume = "24", number = "11", doi = "10.3390/ijms24119384" }
Đekić, S., Vekić, J., Zeljković, A., Kotur-Stevuljević, J., Kafedžić, S., Zdravković, M., Ilić, I., Hinić, S., Cerović, M., Stefanović, M., Mihajlović, M., Nešković, A.,& Bogavac-Stanojević, N.. (2023). HDL Subclasses and the Distribution of Paraoxonase-1 Activity in Patients with ST-Segment Elevation Acute Myocardial Infarction. in International Journal of Molecular Sciences MDPI., 24(11). https://doi.org/10.3390/ijms24119384
Đekić S, Vekić J, Zeljković A, Kotur-Stevuljević J, Kafedžić S, Zdravković M, Ilić I, Hinić S, Cerović M, Stefanović M, Mihajlović M, Nešković A, Bogavac-Stanojević N. HDL Subclasses and the Distribution of Paraoxonase-1 Activity in Patients with ST-Segment Elevation Acute Myocardial Infarction. in International Journal of Molecular Sciences. 2023;24(11). doi:10.3390/ijms24119384 .
Đekić, Saška, Vekić, Jelena, Zeljković, Aleksandra, Kotur-Stevuljević, Jelena, Kafedžić, Srđan, Zdravković, Marija, Ilić, Ivan, Hinić, Saša, Cerović, Milivoje, Stefanović, Milica, Mihajlović, Marija, Nešković, Aleksandar, Bogavac-Stanojević, Nataša, "HDL Subclasses and the Distribution of Paraoxonase-1 Activity in Patients with ST-Segment Elevation Acute Myocardial Infarction" in International Journal of Molecular Sciences, 24, no. 11 (2023), https://doi.org/10.3390/ijms24119384 . .