A simple, rapid and reproducible fluorodensitometric method for the determination of conjugated estrogens has been developed. The proposed procedure includes the following steps: extraction, hydrolysis of sodium sulphate esters of estrone, equilin, equilenin and their 17-α-hydroxy derivatives, separation of the liberated 3-phenolic steroids and in situ measurement of fluorescence. The fluorescence emission was measured after spraying the spots of estrone and estradiol with 2,4-dinitrophenylhydrazine in sulphuric acid-ethanol medium and equilin and 17-α-dihydroequilin with phosphoric acid and sodium hydroxide solution, respectively. Equilenin and 17-α-dihydroequilenin were determined by measuring the native fluorescence. The method applied to the determination of raw material and tablets provided results which agreed well with the stated content and the requirements of USP XXI for conjugated estrogens.
Keywords:
Conjugated estrogens / fluorodensitometric determination / raw material and pharmaceutical preparation.
Source:
Journal of Pharmaceutical and Biomedical Analysis, 1989, 7, 12, 1657-1662
TY - JOUR
AU - Novaković, J
AU - Agbaba, Danica
AU - Stakić, D.Ž
PY - 1989
UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/49
AB - A simple, rapid and reproducible fluorodensitometric method for the determination of conjugated estrogens has been developed. The proposed procedure includes the following steps: extraction, hydrolysis of sodium sulphate esters of estrone, equilin, equilenin and their 17-α-hydroxy derivatives, separation of the liberated 3-phenolic steroids and in situ measurement of fluorescence. The fluorescence emission was measured after spraying the spots of estrone and estradiol with 2,4-dinitrophenylhydrazine in sulphuric acid-ethanol medium and equilin and 17-α-dihydroequilin with phosphoric acid and sodium hydroxide solution, respectively. Equilenin and 17-α-dihydroequilenin were determined by measuring the native fluorescence. The method applied to the determination of raw material and tablets provided results which agreed well with the stated content and the requirements of USP XXI for conjugated estrogens.
PB - Elsevier
T2 - Journal of Pharmaceutical and Biomedical Analysis
T1 - Fluorodensitometric determination of conjugated estrogens in raw material and in pharmaceutical preparations
VL - 7
IS - 12
SP - 1657
EP - 1662
DO - 10.1016/0731-7085(89)80179-7
ER -
@article{
author = "Novaković, J and Agbaba, Danica and Stakić, D.Ž",
year = "1989",
abstract = "A simple, rapid and reproducible fluorodensitometric method for the determination of conjugated estrogens has been developed. The proposed procedure includes the following steps: extraction, hydrolysis of sodium sulphate esters of estrone, equilin, equilenin and their 17-α-hydroxy derivatives, separation of the liberated 3-phenolic steroids and in situ measurement of fluorescence. The fluorescence emission was measured after spraying the spots of estrone and estradiol with 2,4-dinitrophenylhydrazine in sulphuric acid-ethanol medium and equilin and 17-α-dihydroequilin with phosphoric acid and sodium hydroxide solution, respectively. Equilenin and 17-α-dihydroequilenin were determined by measuring the native fluorescence. The method applied to the determination of raw material and tablets provided results which agreed well with the stated content and the requirements of USP XXI for conjugated estrogens.",
publisher = "Elsevier",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Fluorodensitometric determination of conjugated estrogens in raw material and in pharmaceutical preparations",
volume = "7",
number = "12",
pages = "1657-1662",
doi = "10.1016/0731-7085(89)80179-7"
}
Novaković, J., Agbaba, D.,& Stakić, D.Ž. (1989). Fluorodensitometric determination of conjugated estrogens in raw material and in pharmaceutical preparations. in Journal of Pharmaceutical and Biomedical Analysis
Elsevier., 7(12), 1657-1662.
https://doi.org/10.1016/0731-7085(89)80179-7
Novaković J, Agbaba D, Stakić D. Fluorodensitometric determination of conjugated estrogens in raw material and in pharmaceutical preparations. in Journal of Pharmaceutical and Biomedical Analysis. 1989;7(12):1657-1662.
doi:10.1016/0731-7085(89)80179-7 .
Novaković, J, Agbaba, Danica, Stakić, D.Ž, "Fluorodensitometric determination of conjugated estrogens in raw material and in pharmaceutical preparations" in Journal of Pharmaceutical and Biomedical Analysis, 7, no. 12 (1989):1657-1662,
https://doi.org/10.1016/0731-7085(89)80179-7 . .
