Optimization and validation of a hydrophilic interaction liquid chromatography method for determination of moxonidine and its impurities
Апстракт
Fast and simple hydrophilic interactions liquid chromatography method was developed and validated for the analysis of moxonidine and its impurities in pharmaceutical dosage form. The separation was performed on Zorbax RX-SIL column (250 mm x 4.6 mm, 5 µm) using mixture of acetonitrile and 40 mM ammonium-formiat buffer (pH 2.8) in ratio 80:20 (v/v) as mobile phase at flow rate of 1 mL/min, detection at 255 nm and temperature of 25 °C. Under the selected chromatographic conditions separation and analysis of five examined compounds in the mixture is enable within 12 minutes. The validation criteria for selectivity, linearity (r≥0.9976), accuracy (recovery: 93.66 %-114.08 %), precision (RSD: 0.56%-2.55%) and robustness of the method were fulfilled. The obtained values of the limit of detection and quantifiqation revealed that the method can be used for determination of impurities levels below 0.1%.
Извор:
PHYSICAL CHEMISTRY 2016 (Proceedings) 13th International Conference on Fundamental and Applied Aspects of Physical Chemistry, September 26-30, 2016, Belgrade, Serbia, 2016, II, 821-824Издавач:
- Society of Physical Chemists of Serbia
Финансирање / пројекти:
- Синтеза, квантитативни однос између структуре и дејства, физичко-хемијска карактеризација и анализа фармаколошки активних супстанци (RS-MESTD-Basic Research (BR or ON)-172033)
Напомена:
- 13th International Conference on Fundamental and Applied Aspects of Physical Chemistry, September 26-30, 2016, Belgrade, Serbia
Институција/група
PharmacyTY - CONF AU - Filipić, Slavica AU - Elek, Milica AU - Popović, Marija AU - Ružić, Dušan AU - Nikolić, Katarina AU - Agbaba, Danica PY - 2016 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4922 AB - Fast and simple hydrophilic interactions liquid chromatography method was developed and validated for the analysis of moxonidine and its impurities in pharmaceutical dosage form. The separation was performed on Zorbax RX-SIL column (250 mm x 4.6 mm, 5 µm) using mixture of acetonitrile and 40 mM ammonium-formiat buffer (pH 2.8) in ratio 80:20 (v/v) as mobile phase at flow rate of 1 mL/min, detection at 255 nm and temperature of 25 °C. Under the selected chromatographic conditions separation and analysis of five examined compounds in the mixture is enable within 12 minutes. The validation criteria for selectivity, linearity (r≥0.9976), accuracy (recovery: 93.66 %-114.08 %), precision (RSD: 0.56%-2.55%) and robustness of the method were fulfilled. The obtained values of the limit of detection and quantifiqation revealed that the method can be used for determination of impurities levels below 0.1%. PB - Society of Physical Chemists of Serbia C3 - PHYSICAL CHEMISTRY 2016 (Proceedings) 13th International Conference on Fundamental and Applied Aspects of Physical Chemistry, September 26-30, 2016, Belgrade, Serbia T1 - Optimization and validation of a hydrophilic interaction liquid chromatography method for determination of moxonidine and its impurities VL - II SP - 821 EP - 824 UR - https://hdl.handle.net/21.15107/rcub_farfar_4922 ER -
@conference{ author = "Filipić, Slavica and Elek, Milica and Popović, Marija and Ružić, Dušan and Nikolić, Katarina and Agbaba, Danica", year = "2016", abstract = "Fast and simple hydrophilic interactions liquid chromatography method was developed and validated for the analysis of moxonidine and its impurities in pharmaceutical dosage form. The separation was performed on Zorbax RX-SIL column (250 mm x 4.6 mm, 5 µm) using mixture of acetonitrile and 40 mM ammonium-formiat buffer (pH 2.8) in ratio 80:20 (v/v) as mobile phase at flow rate of 1 mL/min, detection at 255 nm and temperature of 25 °C. Under the selected chromatographic conditions separation and analysis of five examined compounds in the mixture is enable within 12 minutes. The validation criteria for selectivity, linearity (r≥0.9976), accuracy (recovery: 93.66 %-114.08 %), precision (RSD: 0.56%-2.55%) and robustness of the method were fulfilled. The obtained values of the limit of detection and quantifiqation revealed that the method can be used for determination of impurities levels below 0.1%.", publisher = "Society of Physical Chemists of Serbia", journal = "PHYSICAL CHEMISTRY 2016 (Proceedings) 13th International Conference on Fundamental and Applied Aspects of Physical Chemistry, September 26-30, 2016, Belgrade, Serbia", title = "Optimization and validation of a hydrophilic interaction liquid chromatography method for determination of moxonidine and its impurities", volume = "II", pages = "821-824", url = "https://hdl.handle.net/21.15107/rcub_farfar_4922" }
Filipić, S., Elek, M., Popović, M., Ružić, D., Nikolić, K.,& Agbaba, D.. (2016). Optimization and validation of a hydrophilic interaction liquid chromatography method for determination of moxonidine and its impurities. in PHYSICAL CHEMISTRY 2016 (Proceedings) 13th International Conference on Fundamental and Applied Aspects of Physical Chemistry, September 26-30, 2016, Belgrade, Serbia Society of Physical Chemists of Serbia., II, 821-824. https://hdl.handle.net/21.15107/rcub_farfar_4922
Filipić S, Elek M, Popović M, Ružić D, Nikolić K, Agbaba D. Optimization and validation of a hydrophilic interaction liquid chromatography method for determination of moxonidine and its impurities. in PHYSICAL CHEMISTRY 2016 (Proceedings) 13th International Conference on Fundamental and Applied Aspects of Physical Chemistry, September 26-30, 2016, Belgrade, Serbia. 2016;II:821-824. https://hdl.handle.net/21.15107/rcub_farfar_4922 .
Filipić, Slavica, Elek, Milica, Popović, Marija, Ružić, Dušan, Nikolić, Katarina, Agbaba, Danica, "Optimization and validation of a hydrophilic interaction liquid chromatography method for determination of moxonidine and its impurities" in PHYSICAL CHEMISTRY 2016 (Proceedings) 13th International Conference on Fundamental and Applied Aspects of Physical Chemistry, September 26-30, 2016, Belgrade, Serbia, II (2016):821-824, https://hdl.handle.net/21.15107/rcub_farfar_4922 .