In vivo and in silico approach in revealing the influence of lead (Pb) on thyroid gland function
Само за регистроване кориснике
2023
Аутори
Vukelić, DraganaBuha-Đorđević, Aleksandra
Anđelković, Milena
Baralić, Katarina
Ćurčić, Marijana
Đukić-Ćosić, Danijela
Antonijević, Biljana
Bulat, Zorica
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The purpose of this study was to examine the impact of low doses of lead (Pb) on levels of thyroid hormones (T3, T4, FT3, and FT4) and thyroid-related antibodies (anti-Tg and anti-TPO) in the rat model, as well as genes that are related to Pb and thyroid function, relationships between genes, biological processes, molecular processes, and pathways using an in silico approach. Male rats were randomized into seven groups (n = 42), one control group and six groups that received a range of Pb doses: 0.1, 0.5, 1, 3, 7, and 15 mg Pb/kg body weight (b.w.). Dose-response modelling was performed by PROAST software using model averaging method. The Comparative Toxicogenomics Database, GeneMANIA server, and ToppGene Suite portal were used as the main bioinformatic tools in this analysis. The results of our study have shown that low Pb doses induced elevation of thyroid hormones (T4, FT4, and TSH) in rats after subacute exposure, while had no impact on T3, FT3, anti-TPO, and anti-Tg, indicating hy...perthyroidism. Dose-dependent effects were increases in T4 and FT4, with the lowest benchmark dose derived for FT4 levels. In silico toxicogenomic data analysis showed that the main molecular pathways/process related to Pb-induced hyperthyroidism are connected with 14 genes involved in antioxidant defense and Se-dependent processes. The results presented here may be useful in further investigation of the health impacts of low-level Pb exposure on thyroid function and endocrine disruption effects.
Кључне речи:
Pb / Toxicogenomic data mining / Hyperthyroidism / Thyroid gland / Thyroid hormonesИзвор:
Environmental Research, 2023, 237Издавач:
- Elsevier
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200161 (Универзитет у Београду, Фармацеутски факултет) (RS-MESTD-inst-2020-200161)
DOI: 10.1016/j.envres.2023.117035
ISSN: 0013-9351
PubMed: 37659646
Scopus: 2-s2.0-85170208329
Институција/група
PharmacyTY - JOUR AU - Vukelić, Dragana AU - Buha-Đorđević, Aleksandra AU - Anđelković, Milena AU - Baralić, Katarina AU - Ćurčić, Marijana AU - Đukić-Ćosić, Danijela AU - Antonijević, Biljana AU - Bulat, Zorica PY - 2023 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5033 AB - The purpose of this study was to examine the impact of low doses of lead (Pb) on levels of thyroid hormones (T3, T4, FT3, and FT4) and thyroid-related antibodies (anti-Tg and anti-TPO) in the rat model, as well as genes that are related to Pb and thyroid function, relationships between genes, biological processes, molecular processes, and pathways using an in silico approach. Male rats were randomized into seven groups (n = 42), one control group and six groups that received a range of Pb doses: 0.1, 0.5, 1, 3, 7, and 15 mg Pb/kg body weight (b.w.). Dose-response modelling was performed by PROAST software using model averaging method. The Comparative Toxicogenomics Database, GeneMANIA server, and ToppGene Suite portal were used as the main bioinformatic tools in this analysis. The results of our study have shown that low Pb doses induced elevation of thyroid hormones (T4, FT4, and TSH) in rats after subacute exposure, while had no impact on T3, FT3, anti-TPO, and anti-Tg, indicating hyperthyroidism. Dose-dependent effects were increases in T4 and FT4, with the lowest benchmark dose derived for FT4 levels. In silico toxicogenomic data analysis showed that the main molecular pathways/process related to Pb-induced hyperthyroidism are connected with 14 genes involved in antioxidant defense and Se-dependent processes. The results presented here may be useful in further investigation of the health impacts of low-level Pb exposure on thyroid function and endocrine disruption effects. PB - Elsevier T2 - Environmental Research T1 - In vivo and in silico approach in revealing the influence of lead (Pb) on thyroid gland function VL - 237 DO - 10.1016/j.envres.2023.117035 ER -
@article{ author = "Vukelić, Dragana and Buha-Đorđević, Aleksandra and Anđelković, Milena and Baralić, Katarina and Ćurčić, Marijana and Đukić-Ćosić, Danijela and Antonijević, Biljana and Bulat, Zorica", year = "2023", abstract = "The purpose of this study was to examine the impact of low doses of lead (Pb) on levels of thyroid hormones (T3, T4, FT3, and FT4) and thyroid-related antibodies (anti-Tg and anti-TPO) in the rat model, as well as genes that are related to Pb and thyroid function, relationships between genes, biological processes, molecular processes, and pathways using an in silico approach. Male rats were randomized into seven groups (n = 42), one control group and six groups that received a range of Pb doses: 0.1, 0.5, 1, 3, 7, and 15 mg Pb/kg body weight (b.w.). Dose-response modelling was performed by PROAST software using model averaging method. The Comparative Toxicogenomics Database, GeneMANIA server, and ToppGene Suite portal were used as the main bioinformatic tools in this analysis. The results of our study have shown that low Pb doses induced elevation of thyroid hormones (T4, FT4, and TSH) in rats after subacute exposure, while had no impact on T3, FT3, anti-TPO, and anti-Tg, indicating hyperthyroidism. Dose-dependent effects were increases in T4 and FT4, with the lowest benchmark dose derived for FT4 levels. In silico toxicogenomic data analysis showed that the main molecular pathways/process related to Pb-induced hyperthyroidism are connected with 14 genes involved in antioxidant defense and Se-dependent processes. The results presented here may be useful in further investigation of the health impacts of low-level Pb exposure on thyroid function and endocrine disruption effects.", publisher = "Elsevier", journal = "Environmental Research", title = "In vivo and in silico approach in revealing the influence of lead (Pb) on thyroid gland function", volume = "237", doi = "10.1016/j.envres.2023.117035" }
Vukelić, D., Buha-Đorđević, A., Anđelković, M., Baralić, K., Ćurčić, M., Đukić-Ćosić, D., Antonijević, B.,& Bulat, Z.. (2023). In vivo and in silico approach in revealing the influence of lead (Pb) on thyroid gland function. in Environmental Research Elsevier., 237. https://doi.org/10.1016/j.envres.2023.117035
Vukelić D, Buha-Đorđević A, Anđelković M, Baralić K, Ćurčić M, Đukić-Ćosić D, Antonijević B, Bulat Z. In vivo and in silico approach in revealing the influence of lead (Pb) on thyroid gland function. in Environmental Research. 2023;237. doi:10.1016/j.envres.2023.117035 .
Vukelić, Dragana, Buha-Đorđević, Aleksandra, Anđelković, Milena, Baralić, Katarina, Ćurčić, Marijana, Đukić-Ćosić, Danijela, Antonijević, Biljana, Bulat, Zorica, "In vivo and in silico approach in revealing the influence of lead (Pb) on thyroid gland function" in Environmental Research, 237 (2023), https://doi.org/10.1016/j.envres.2023.117035 . .