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Retention modelling in liquid chromatographic separation of simvastatin and six impurities using a microemulsion as eluent

Authorized Users Only
2004
Authors
Malenović, Anđelija
Ivanović, D
Medenica, Mirjana
Jančić, Biljana
Marković, S
Article (Published version)
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Abstract
A novel and unique approach was used for retention modelling in the separation of simvastatin and six impurities by liquid chromatographic using a microemulsion as mobile phase. A microemulsion is a modification of a micellar system where a lipophilic organic solvent is dissolved in the micelles; for that reason, microemulsions are usually treated as solvent-modified micellar solutions. When microemulsions are used as eluents in HPLC separations, solutes partition between the charged oil droplets and the aqueous buffer phase. The complexity of the composition of the microemulsion permits extensive manipulations to be made during method development in order to achieve acceptable resolution of such a complex mixture of substances. In order to avoid a laborious "trial and error" procedure, a 2(3) full factorial design was applied for choosing an optimal microemulsion composition to obtain good separation in a reasonable run time. Organic solvent, sodium dodecyl sulphate, and n-butanol con...tent were varied within defined experimental domain. Optimal conditions for the separation of simvastatin and its six impurities were obtained using an X Terra(TM) 50 x 4.6 mm, 3.5 mum particle size column at 30degreesC. The mobile phase consisted of 0.9% w/w of diisopropyl ether, 2.2% w/w of sodium dodecylsulphate (SDS), 7.0% w/w of co-surfactant such as n-butanol, and 89.9% w/w of aqueous 25 mM disodium phosphate pH 7.0.

Keywords:
simvastatin / impurities / LC / microemulsion
Source:
Journal of Separation Science, 2004, 27, 13, 1087-1092
Publisher:
  • Wiley-VCH Verlag GMBH, Weinheim

DOI: 10.1002/jssc.200401748

ISSN: 1615-9306

PubMed: 15495410

WoS: 000224188000004

Scopus: 2-s2.0-4844223516
[ Google Scholar ]
48
40
URI
https://farfar.pharmacy.bg.ac.rs/handle/123456789/505
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Pharmacy
TY  - JOUR
AU  - Malenović, Anđelija
AU  - Ivanović, D
AU  - Medenica, Mirjana
AU  - Jančić, Biljana
AU  - Marković, S
PY  - 2004
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/505
AB  - A novel and unique approach was used for retention modelling in the separation of simvastatin and six impurities by liquid chromatographic using a microemulsion as mobile phase. A microemulsion is a modification of a micellar system where a lipophilic organic solvent is dissolved in the micelles; for that reason, microemulsions are usually treated as solvent-modified micellar solutions. When microemulsions are used as eluents in HPLC separations, solutes partition between the charged oil droplets and the aqueous buffer phase. The complexity of the composition of the microemulsion permits extensive manipulations to be made during method development in order to achieve acceptable resolution of such a complex mixture of substances. In order to avoid a laborious "trial and error" procedure, a 2(3) full factorial design was applied for choosing an optimal microemulsion composition to obtain good separation in a reasonable run time. Organic solvent, sodium dodecyl sulphate, and n-butanol content were varied within defined experimental domain. Optimal conditions for the separation of simvastatin and its six impurities were obtained using an X Terra(TM) 50 x 4.6 mm, 3.5 mum particle size column at 30degreesC. The mobile phase consisted of 0.9% w/w of diisopropyl ether, 2.2% w/w of sodium dodecylsulphate (SDS), 7.0% w/w of co-surfactant such as n-butanol, and 89.9% w/w of aqueous 25 mM disodium phosphate pH 7.0.
PB  - Wiley-VCH Verlag GMBH, Weinheim
T2  - Journal of Separation Science
T1  - Retention modelling in liquid chromatographic separation of simvastatin and six impurities using a microemulsion as eluent
VL  - 27
IS  - 13
SP  - 1087
EP  - 1092
DO  - 10.1002/jssc.200401748
ER  - 
@article{
author = "Malenović, Anđelija and Ivanović, D and Medenica, Mirjana and Jančić, Biljana and Marković, S",
year = "2004",
abstract = "A novel and unique approach was used for retention modelling in the separation of simvastatin and six impurities by liquid chromatographic using a microemulsion as mobile phase. A microemulsion is a modification of a micellar system where a lipophilic organic solvent is dissolved in the micelles; for that reason, microemulsions are usually treated as solvent-modified micellar solutions. When microemulsions are used as eluents in HPLC separations, solutes partition between the charged oil droplets and the aqueous buffer phase. The complexity of the composition of the microemulsion permits extensive manipulations to be made during method development in order to achieve acceptable resolution of such a complex mixture of substances. In order to avoid a laborious "trial and error" procedure, a 2(3) full factorial design was applied for choosing an optimal microemulsion composition to obtain good separation in a reasonable run time. Organic solvent, sodium dodecyl sulphate, and n-butanol content were varied within defined experimental domain. Optimal conditions for the separation of simvastatin and its six impurities were obtained using an X Terra(TM) 50 x 4.6 mm, 3.5 mum particle size column at 30degreesC. The mobile phase consisted of 0.9% w/w of diisopropyl ether, 2.2% w/w of sodium dodecylsulphate (SDS), 7.0% w/w of co-surfactant such as n-butanol, and 89.9% w/w of aqueous 25 mM disodium phosphate pH 7.0.",
publisher = "Wiley-VCH Verlag GMBH, Weinheim",
journal = "Journal of Separation Science",
title = "Retention modelling in liquid chromatographic separation of simvastatin and six impurities using a microemulsion as eluent",
volume = "27",
number = "13",
pages = "1087-1092",
doi = "10.1002/jssc.200401748"
}
Malenović, A., Ivanović, D., Medenica, M., Jančić, B.,& Marković, S.. (2004). Retention modelling in liquid chromatographic separation of simvastatin and six impurities using a microemulsion as eluent. in Journal of Separation Science
Wiley-VCH Verlag GMBH, Weinheim., 27(13), 1087-1092.
https://doi.org/10.1002/jssc.200401748
Malenović A, Ivanović D, Medenica M, Jančić B, Marković S. Retention modelling in liquid chromatographic separation of simvastatin and six impurities using a microemulsion as eluent. in Journal of Separation Science. 2004;27(13):1087-1092.
doi:10.1002/jssc.200401748 .
Malenović, Anđelija, Ivanović, D, Medenica, Mirjana, Jančić, Biljana, Marković, S, "Retention modelling in liquid chromatographic separation of simvastatin and six impurities using a microemulsion as eluent" in Journal of Separation Science, 27, no. 13 (2004):1087-1092,
https://doi.org/10.1002/jssc.200401748 . .

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