Phenolic profile and in vitro biological activities of blackthorn fruit (Prunus spinosa L.)
Апстракт
The blackthorn, Prunus spinosa L. (Rosaceae) is a continental shrub widespread in Europe. The fruit is used in traditional medicine in the treatment of respiratory disorders, as well as diuretic, spasmolytic and anti-inflammatory agent. The aim of this study was to investigate the phenolic profile and in vitro biological activities: antioxidant, anti-diabetic, anti-tyrosinase, and anti-acetylcholinesterase. Furthermore the effect of blackthorn fruit extract on probiotic microorganisms was also studied. The blackthorn fruits were collected from two localities in Serbia and extracted with water, methanol and ethanol (50% V/V) at room temperature. The total phenolic contents was analysed by the Folin-Ciocalteu assay and was the highest in methanol extracts (321.36±9.13 and 217.04±17.99 mg GAE/100 g). On the contrary ethanol extracts contained the highest total flavonoids content (67.88±1.05 and 39.70±3.19 mg CE/100 g). Hydroxycinnamic acid derivatives (caffeoylquinic acid, caffeoyl hexosi...de) and quercetin glycosides (rutin, quercetin pentosyl-hexoside, isoquercitrin, quercetin pentoside) were detected in methanol extracts of both samples by LC-MS. Blackthorn ethanol extracts showed pronounced ferric reducing activity in FRAP test (2.80±0.07 and 1.89±0.01 mmol Trolox /100 g) and highest ability to neutralise ABTS radical (41.02±0.77 and 45.84±1.01 mmol Trolox /100 g). On the other hand methanol extract of both samples exerted significant DPPH radical scavenging activity (3.38±0.1 and 2.61±0.17 mmol Trolox/100 g) and highest activity in β-carotene/linoleic acid bleaching test (20.89±2.28 and 21.16±2.43%). Methanol extract of both samples inhibited the enzimes: α-amylase (IC50 2.05±0.05 and 1.26±0.04 mg/ml) α-glucosidase (IC50 0.63±0.02 and 0.43±0.06 mg/ml), tyrosinase (IC50 1.0±0.07 and 0.57±0.02 mg/ml), and acetylcholinesterase (IC50 0.56±0.28 and 2.16±0.56 mg/ml). In addition the methanol extracts of both samples markedly stimulated the growth of investigated probiotic microorganisms.
The blackthorn fruit represents a rich source of biologically active compounds and although it is almost forgotten it should be used again in human nutrition to maintain health.
Кључне речи:
Prunus spinosa / fruit / phenolics / antioxidant / enzyme inhibitory activityИзвор:
UNIFOOD conference, Belgrade, September 24-25, 2021, Book of Abstracts, 2021, 85-85Издавач:
- University of Belgrade
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200161 (Универзитет у Београду, Фармацеутски факултет) (RS-MESTD-inst-2020-200161)
Напомена:
- UNIFood2021 Conference, 24th-25th September 2021 University of Belgrade, 2nd International UNIfood Conference
Институција/група
PharmacyTY - CONF AU - Marčetić, Mirjana AU - Samardžić, Stevan AU - Ilić, Tijana AU - Božić, Dragana AU - Vidović, Bojana PY - 2021 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5097 AB - The blackthorn, Prunus spinosa L. (Rosaceae) is a continental shrub widespread in Europe. The fruit is used in traditional medicine in the treatment of respiratory disorders, as well as diuretic, spasmolytic and anti-inflammatory agent. The aim of this study was to investigate the phenolic profile and in vitro biological activities: antioxidant, anti-diabetic, anti-tyrosinase, and anti-acetylcholinesterase. Furthermore the effect of blackthorn fruit extract on probiotic microorganisms was also studied. The blackthorn fruits were collected from two localities in Serbia and extracted with water, methanol and ethanol (50% V/V) at room temperature. The total phenolic contents was analysed by the Folin-Ciocalteu assay and was the highest in methanol extracts (321.36±9.13 and 217.04±17.99 mg GAE/100 g). On the contrary ethanol extracts contained the highest total flavonoids content (67.88±1.05 and 39.70±3.19 mg CE/100 g). Hydroxycinnamic acid derivatives (caffeoylquinic acid, caffeoyl hexoside) and quercetin glycosides (rutin, quercetin pentosyl-hexoside, isoquercitrin, quercetin pentoside) were detected in methanol extracts of both samples by LC-MS. Blackthorn ethanol extracts showed pronounced ferric reducing activity in FRAP test (2.80±0.07 and 1.89±0.01 mmol Trolox /100 g) and highest ability to neutralise ABTS radical (41.02±0.77 and 45.84±1.01 mmol Trolox /100 g). On the other hand methanol extract of both samples exerted significant DPPH radical scavenging activity (3.38±0.1 and 2.61±0.17 mmol Trolox/100 g) and highest activity in β-carotene/linoleic acid bleaching test (20.89±2.28 and 21.16±2.43%). Methanol extract of both samples inhibited the enzimes: α-amylase (IC50 2.05±0.05 and 1.26±0.04 mg/ml) α-glucosidase (IC50 0.63±0.02 and 0.43±0.06 mg/ml), tyrosinase (IC50 1.0±0.07 and 0.57±0.02 mg/ml), and acetylcholinesterase (IC50 0.56±0.28 and 2.16±0.56 mg/ml). In addition the methanol extracts of both samples markedly stimulated the growth of investigated probiotic microorganisms. The blackthorn fruit represents a rich source of biologically active compounds and although it is almost forgotten it should be used again in human nutrition to maintain health. PB - University of Belgrade C3 - UNIFOOD conference, Belgrade, September 24-25, 2021, Book of Abstracts T1 - Phenolic profile and in vitro biological activities of blackthorn fruit (Prunus spinosa L.) SP - 85 EP - 85 UR - https://hdl.handle.net/21.15107/rcub_farfar_5097 ER -
@conference{ author = "Marčetić, Mirjana and Samardžić, Stevan and Ilić, Tijana and Božić, Dragana and Vidović, Bojana", year = "2021", abstract = "The blackthorn, Prunus spinosa L. (Rosaceae) is a continental shrub widespread in Europe. The fruit is used in traditional medicine in the treatment of respiratory disorders, as well as diuretic, spasmolytic and anti-inflammatory agent. The aim of this study was to investigate the phenolic profile and in vitro biological activities: antioxidant, anti-diabetic, anti-tyrosinase, and anti-acetylcholinesterase. Furthermore the effect of blackthorn fruit extract on probiotic microorganisms was also studied. The blackthorn fruits were collected from two localities in Serbia and extracted with water, methanol and ethanol (50% V/V) at room temperature. The total phenolic contents was analysed by the Folin-Ciocalteu assay and was the highest in methanol extracts (321.36±9.13 and 217.04±17.99 mg GAE/100 g). On the contrary ethanol extracts contained the highest total flavonoids content (67.88±1.05 and 39.70±3.19 mg CE/100 g). Hydroxycinnamic acid derivatives (caffeoylquinic acid, caffeoyl hexoside) and quercetin glycosides (rutin, quercetin pentosyl-hexoside, isoquercitrin, quercetin pentoside) were detected in methanol extracts of both samples by LC-MS. Blackthorn ethanol extracts showed pronounced ferric reducing activity in FRAP test (2.80±0.07 and 1.89±0.01 mmol Trolox /100 g) and highest ability to neutralise ABTS radical (41.02±0.77 and 45.84±1.01 mmol Trolox /100 g). On the other hand methanol extract of both samples exerted significant DPPH radical scavenging activity (3.38±0.1 and 2.61±0.17 mmol Trolox/100 g) and highest activity in β-carotene/linoleic acid bleaching test (20.89±2.28 and 21.16±2.43%). Methanol extract of both samples inhibited the enzimes: α-amylase (IC50 2.05±0.05 and 1.26±0.04 mg/ml) α-glucosidase (IC50 0.63±0.02 and 0.43±0.06 mg/ml), tyrosinase (IC50 1.0±0.07 and 0.57±0.02 mg/ml), and acetylcholinesterase (IC50 0.56±0.28 and 2.16±0.56 mg/ml). In addition the methanol extracts of both samples markedly stimulated the growth of investigated probiotic microorganisms. The blackthorn fruit represents a rich source of biologically active compounds and although it is almost forgotten it should be used again in human nutrition to maintain health.", publisher = "University of Belgrade", journal = "UNIFOOD conference, Belgrade, September 24-25, 2021, Book of Abstracts", title = "Phenolic profile and in vitro biological activities of blackthorn fruit (Prunus spinosa L.)", pages = "85-85", url = "https://hdl.handle.net/21.15107/rcub_farfar_5097" }
Marčetić, M., Samardžić, S., Ilić, T., Božić, D.,& Vidović, B.. (2021). Phenolic profile and in vitro biological activities of blackthorn fruit (Prunus spinosa L.). in UNIFOOD conference, Belgrade, September 24-25, 2021, Book of Abstracts University of Belgrade., 85-85. https://hdl.handle.net/21.15107/rcub_farfar_5097
Marčetić M, Samardžić S, Ilić T, Božić D, Vidović B. Phenolic profile and in vitro biological activities of blackthorn fruit (Prunus spinosa L.). in UNIFOOD conference, Belgrade, September 24-25, 2021, Book of Abstracts. 2021;:85-85. https://hdl.handle.net/21.15107/rcub_farfar_5097 .
Marčetić, Mirjana, Samardžić, Stevan, Ilić, Tijana, Božić, Dragana, Vidović, Bojana, "Phenolic profile and in vitro biological activities of blackthorn fruit (Prunus spinosa L.)" in UNIFOOD conference, Belgrade, September 24-25, 2021, Book of Abstracts (2021):85-85, https://hdl.handle.net/21.15107/rcub_farfar_5097 .