FarFaR - Pharmacy Repository
University of Belgrade, Faculty of Pharmacy
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrilic)
    • Serbian (Latin)
  • Login
View Item 
  •   FarFaR
  • Pharmacy
  • Radovi istraživača / Researchers’ publications
  • View Item
  •   FarFaR
  • Pharmacy
  • Radovi istraživača / Researchers’ publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Stability testing of cefuroxime in tablets by micellar liquid chromatography

Authorized Users Only
2004
Authors
Živanović, L
Ivanović, I
Solomun, Ljiljana
Zečević, Mira
Article (Published version)
Metadata
Show full item record
Abstract
Cefuroxime is a broad-spectrum second-generation bactericidal cephalosporin antibiotic active against beta-lactamose-producing strains. Anti-cefuroxime, the geometric isomer of cefuroxime, might be present in cefuroxime dosage forms as a process-related impurity and possible degradation product. In the work discussed in this paper a precise and sensitive micellar liquid chromatographic (MLC) method for stability testing of cefuroxime axetil and anti-cefuroxime axetil in tablets, using benzoic acid as internal standard, was developed and validated. MLC was performed on an XTerra C-18 reversed-phase column at 50 degreesC with 8:92 (v/v) acetonitrile-20 mm sodium dodecyl sulphate, pH 2.5, as mobile phase at a flow rate of 1.5 mL min(-1). Detection was at 280 nm. Under these conditions the retention time and retention factor were of 6.65 min and 4.57, respectively, for cefuroxime axetil and 11.45 min and 8.59, respectively, for anti-cefuroxime axetil, indicating that the compounds were wel...l separated. RSD values for quantification of cefuroxime axetil and anti-cefuroxime axetil were 0.39 and 1.7%, respectively, indicating the precision of the MLC method was good. The method is sensitive-LOD = 0.5 mug mL(-1) and LOQ = 1.5 mug mL(-1) for anti-cefuroxime axetil-and reproducible, with good recovery values.

Keywords:
micellar liquid chromatography / stability testing / cefuroxime axetil / anti-cefuroxime axetil
Source:
Chromatographia, 2004, 60, SUPPL.
Publisher:
  • Springer Heidelberg, Heidelberg

DOI: 10.1365/s10337-004-0237-5

ISSN: 0009-5893

WoS: 000224155700009

Scopus: 2-s2.0-3943102031
[ Google Scholar ]
10
9
URI
http://farfar.pharmacy.bg.ac.rs/handle/123456789/510
Collections
  • Radovi istraživača / Researchers’ publications
Institution
Pharmacy

DSpace software copyright © 2002-2015  DuraSpace
About FarFaR - Pharmacy Repository | Send Feedback

OpenAIRERCUB
 

 

All of DSpaceInstitutionsAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About FarFaR - Pharmacy Repository | Send Feedback

OpenAIRERCUB