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dc.creatorMaksimović, Zoran
dc.creatorMalenović, Anđelija
dc.creatorJančić, Biljana
dc.creatorKovačević, Nada
dc.date.accessioned2019-09-02T10:59:01Z
dc.date.available2019-09-02T10:59:01Z
dc.date.issued2004
dc.identifier.issn0031-7144
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/514
dc.description.abstractA RP-HPLC method for quantification of allantoin in silk of fifteen maize hybrids (Zea mays L., Poaceae) was described. Following extraction of the plant material with an acetone-water (7:3, V/V) mixture, filtration and dilution, the extracts were analyzed without previous chemical derivatization. Separation and quantification were achieved using an Alltech Econosil C18 column under isocratic conditions at 40 degreesC. The mobile phase flow (20% methanol -80% water with 5 mM sodium laurylsulfate added at pH 2.5, adjusted with 85% orthophosphoric acid; pH of water phase was finally adjusted at 6.0 by addition of triethylamine) was maintained at 1.0 mL/min. Column effluent was monitored at 235 nm. This simple procedure afforded efficient separation and quantification of allantoin in plant material, without interference of polyphenols or other plant constituents of medium to high polarity, or similar UV absorption. Our study revealed that the silk of all investigated maize hybrids could be considered relatively rich in allantoin, covering the concentration range between 215 and 289 mg per 100 g of dry plant material.en
dc.publisherGovi-Verlag Pharmazeutischer Verlag Gmbh, Eschborn
dc.rightsrestrictedAccess
dc.sourcePharmazie
dc.titleQuantification of allantoin in various Zea mays L. hybrids by RP-HPLC with UV detectionen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractЈанчић, Биљана; Ковачевић, Нада; Маленовић, Aнђелија; Максимовић, Зоран;
dc.citation.volume59
dc.citation.issue7
dc.citation.spage524
dc.citation.epage527
dc.citation.other59(7): 524-527
dc.citation.rankM23
dc.identifier.wos000222695500006
dc.identifier.pmid15296088
dc.identifier.scopus2-s2.0-3242704255
dc.identifier.rcubconv_1551
dc.type.versionpublishedVersion


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