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Application of the response surface methodology for RP-HPLC analysis of lidocaine and cetrimonium bromide

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Authors
Malenović, Anđelija
Ivanović, D
Medenica, Mirjana
Jančić, Biljana
Article (Published version)
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Abstract
The successful analysis of drugs in complex pharmaceutical formulations by reversed-phase high performance liquid chromatography (RP-HPLC) relies upon the optimization of the chromatographic conditions, the sample preparation and post-column detection. HPLC separation of mixtures depends on a large number of factors. The optimization of RP-HPLC method defines the simultaneous influence of some important conditions, such as column type, mobile phase composition, pH of the mobile phase, column temperature and flow rate, to the separation and determination. In this paper the RP-HPLC method was applied for the optimal separation of lidocaine, cetrimonium bromide and color in the pharmaceutical pellets (Septalen(R) pellets). The selectivity factor values defined the optimal conditions, which were confirmed by analyzing the appropriate mathematical models. With the aid of the response surface methodology (RSM) it was possible to anticipate to a certain degree and precisely select optimal exp...erimental conditions. Separations were performed on a Beckman Ultrasphere ODS 4.6 x 150 mm, 5 mum particle size column. Samples were introduced through a Rheodyne injector valve with a 20 muL sample loop. UV detection was performed at 208 nm. The optimization was performed within the pH range from 2.0 to 5.5; temperature range from 20 degrees C to 55 degrees C and composition of the mobile phase acetonitrile water from (15:85 V/V) to (35:65 V/V). The three-D graphs, constructed with sixty-four experimental points, confirmed the optimal conditions for the separation of lidocaine, cetrimonium bromide and color in analyzed pharmaceutical preparations.

Keywords:
lidocaine / cetrimonium bromide / RP-HPLC / RSM / optimization
Source:
Acta Chimica Slovenica, 2004, 51, 3, 559-566
Publisher:
  • Slovensko Kemijsko Drustvo, Ljubljana

ISSN: 1318-0207

WoS: 000223995600016

Scopus: 2-s2.0-6444235608
[ Google Scholar ]
7
7
Handle
https://hdl.handle.net/21.15107/rcub_farfar_522
URI
https://farfar.pharmacy.bg.ac.rs/handle/123456789/522
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Pharmacy
TY  - JOUR
AU  - Malenović, Anđelija
AU  - Ivanović, D
AU  - Medenica, Mirjana
AU  - Jančić, Biljana
PY  - 2004
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/522
AB  - The successful analysis of drugs in complex pharmaceutical formulations by reversed-phase high performance liquid chromatography (RP-HPLC) relies upon the optimization of the chromatographic conditions, the sample preparation and post-column detection. HPLC separation of mixtures depends on a large number of factors. The optimization of RP-HPLC method defines the simultaneous influence of some important conditions, such as column type, mobile phase composition, pH of the mobile phase, column temperature and flow rate, to the separation and determination. In this paper the RP-HPLC method was applied for the optimal separation of lidocaine, cetrimonium bromide and color in the pharmaceutical pellets (Septalen(R) pellets). The selectivity factor values defined the optimal conditions, which were confirmed by analyzing the appropriate mathematical models. With the aid of the response surface methodology (RSM) it was possible to anticipate to a certain degree and precisely select optimal experimental conditions. Separations were performed on a Beckman Ultrasphere ODS 4.6 x 150 mm, 5 mum particle size column. Samples were introduced through a Rheodyne injector valve with a 20 muL sample loop. UV detection was performed at 208 nm. The optimization was performed within the pH range from 2.0 to 5.5; temperature range from 20 degrees C to 55 degrees C and composition of the mobile phase acetonitrile water from (15:85 V/V) to (35:65 V/V). The three-D graphs, constructed with sixty-four experimental points, confirmed the optimal conditions for the separation of lidocaine, cetrimonium bromide and color in analyzed pharmaceutical preparations.
PB  - Slovensko Kemijsko Drustvo, Ljubljana
T2  - Acta Chimica Slovenica
T1  - Application of the response surface methodology for RP-HPLC analysis of lidocaine and cetrimonium bromide
VL  - 51
IS  - 3
SP  - 559
EP  - 566
UR  - https://hdl.handle.net/21.15107/rcub_farfar_522
ER  - 
@article{
author = "Malenović, Anđelija and Ivanović, D and Medenica, Mirjana and Jančić, Biljana",
year = "2004",
abstract = "The successful analysis of drugs in complex pharmaceutical formulations by reversed-phase high performance liquid chromatography (RP-HPLC) relies upon the optimization of the chromatographic conditions, the sample preparation and post-column detection. HPLC separation of mixtures depends on a large number of factors. The optimization of RP-HPLC method defines the simultaneous influence of some important conditions, such as column type, mobile phase composition, pH of the mobile phase, column temperature and flow rate, to the separation and determination. In this paper the RP-HPLC method was applied for the optimal separation of lidocaine, cetrimonium bromide and color in the pharmaceutical pellets (Septalen(R) pellets). The selectivity factor values defined the optimal conditions, which were confirmed by analyzing the appropriate mathematical models. With the aid of the response surface methodology (RSM) it was possible to anticipate to a certain degree and precisely select optimal experimental conditions. Separations were performed on a Beckman Ultrasphere ODS 4.6 x 150 mm, 5 mum particle size column. Samples were introduced through a Rheodyne injector valve with a 20 muL sample loop. UV detection was performed at 208 nm. The optimization was performed within the pH range from 2.0 to 5.5; temperature range from 20 degrees C to 55 degrees C and composition of the mobile phase acetonitrile water from (15:85 V/V) to (35:65 V/V). The three-D graphs, constructed with sixty-four experimental points, confirmed the optimal conditions for the separation of lidocaine, cetrimonium bromide and color in analyzed pharmaceutical preparations.",
publisher = "Slovensko Kemijsko Drustvo, Ljubljana",
journal = "Acta Chimica Slovenica",
title = "Application of the response surface methodology for RP-HPLC analysis of lidocaine and cetrimonium bromide",
volume = "51",
number = "3",
pages = "559-566",
url = "https://hdl.handle.net/21.15107/rcub_farfar_522"
}
Malenović, A., Ivanović, D., Medenica, M.,& Jančić, B.. (2004). Application of the response surface methodology for RP-HPLC analysis of lidocaine and cetrimonium bromide. in Acta Chimica Slovenica
Slovensko Kemijsko Drustvo, Ljubljana., 51(3), 559-566.
https://hdl.handle.net/21.15107/rcub_farfar_522
Malenović A, Ivanović D, Medenica M, Jančić B. Application of the response surface methodology for RP-HPLC analysis of lidocaine and cetrimonium bromide. in Acta Chimica Slovenica. 2004;51(3):559-566.
https://hdl.handle.net/21.15107/rcub_farfar_522 .
Malenović, Anđelija, Ivanović, D, Medenica, Mirjana, Jančić, Biljana, "Application of the response surface methodology for RP-HPLC analysis of lidocaine and cetrimonium bromide" in Acta Chimica Slovenica, 51, no. 3 (2004):559-566,
https://hdl.handle.net/21.15107/rcub_farfar_522 .

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