Anatomy and composition of the essential oil of Larix decidua L. (Pinaceae)
Апстракт
Larix decidua L. is a deciduous coniferous native to the mountains of central Europe, in the Alps and Carpathians. The aim
was to investigate anatomy and the chemical composition of
the essential oils of needles, stems and strobilus of L. decidua.
The samples were collected from larch trees from cultivated
conditions (Belgrade, Serbia.) and from natural habitat (Alps,
Austria). Needles of L. decidua, on cross section are more or
less elliptically shaped. Two small secretory canals are in the
mesophyll of the needles, on their lateral sides, just below the
epidermis. The young stem (primary structures) have canals in cortex, some are just below the epidermis and many, that
are in forming phase above the phloem. The older stem (perennial stem) has many secretory canals in the parenchyma of
cortex and in the xylem of secondary wood. The essential oils
were obtained by hydrodistillation and qualitative and quantitative analysis was performed by GC-FID and GC-MS. Small
amounts o...f essential oils were extracted from the needles
(0.06-0.41%), stems (0.31-0.54%) and strobilus (0.16 % and
0.19 %) of L. decidua. The monoterpene (28.70-50.40%) and
sesquiterpene compounds (41.80-67.20%) dominated in the
essential oil of needles, monoterpenes (80.50-91.70%) in the
oil of stems, diterpenes (46.50% and 40.50%) and monoterpenes (24.20% and 22.20%) in the oil of strobilus. Main com-
pounds in the essential oil of needles represented germacrene
D (15.2-46.9%), δ-3-carene (4.6-24.6%) and α-pinene (6.3-
14.1%), in the oil of stems α-pinene (12.8-21.8%), δ-3-carene
(8.6-14.3%) and sabinene (0.5-10.3%), while in the oil of strobilus diterpene of abietane type (4.1-10.2%) and nor-abietatetraene (3.9-9.2%) were the most abundant. The larch needles
and stem essential oils from cultivated and natural habitats
were quite similar in qualitative composition. However, the
essential oil of needles from natural habitat contained higher
content of germacrene D and α-pinene and the oil of stem
α-pinene and trans-verbenol. The oil of larch strobilus from
natural habitat was characterized by manool and 3-α-acetoxy-manool, diterpenes that were not present in the oil of
cultivated sample.
Кључне речи:
Larix decidua / anatomy / needles / stem / strobilus / essential oilИзвор:
Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts, 2018, 42, 1 supplement, 129-130Издавач:
- Institute of Botany and Botanical Garden Jevremovac, Belgrade
Финансирање / пројекти:
- Испитивање лековитог потенцијала биљака: морфолошка, хемијска и фармаколошка карактеризација (RS-MESTD-Basic Research (BR or ON)-173021)
Напомена:
- 7th Balkan Botanical Congress, 10-14th September 2018, Novi Sad, Serbia
Институција/група
PharmacyTY - CONF AU - Lakušić, Branislava AU - Marčetić, Mirjana PY - 2018 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5254 AB - Larix decidua L. is a deciduous coniferous native to the mountains of central Europe, in the Alps and Carpathians. The aim was to investigate anatomy and the chemical composition of the essential oils of needles, stems and strobilus of L. decidua. The samples were collected from larch trees from cultivated conditions (Belgrade, Serbia.) and from natural habitat (Alps, Austria). Needles of L. decidua, on cross section are more or less elliptically shaped. Two small secretory canals are in the mesophyll of the needles, on their lateral sides, just below the epidermis. The young stem (primary structures) have canals in cortex, some are just below the epidermis and many, that are in forming phase above the phloem. The older stem (perennial stem) has many secretory canals in the parenchyma of cortex and in the xylem of secondary wood. The essential oils were obtained by hydrodistillation and qualitative and quantitative analysis was performed by GC-FID and GC-MS. Small amounts of essential oils were extracted from the needles (0.06-0.41%), stems (0.31-0.54%) and strobilus (0.16 % and 0.19 %) of L. decidua. The monoterpene (28.70-50.40%) and sesquiterpene compounds (41.80-67.20%) dominated in the essential oil of needles, monoterpenes (80.50-91.70%) in the oil of stems, diterpenes (46.50% and 40.50%) and monoterpenes (24.20% and 22.20%) in the oil of strobilus. Main com- pounds in the essential oil of needles represented germacrene D (15.2-46.9%), δ-3-carene (4.6-24.6%) and α-pinene (6.3- 14.1%), in the oil of stems α-pinene (12.8-21.8%), δ-3-carene (8.6-14.3%) and sabinene (0.5-10.3%), while in the oil of strobilus diterpene of abietane type (4.1-10.2%) and nor-abietatetraene (3.9-9.2%) were the most abundant. The larch needles and stem essential oils from cultivated and natural habitats were quite similar in qualitative composition. However, the essential oil of needles from natural habitat contained higher content of germacrene D and α-pinene and the oil of stem α-pinene and trans-verbenol. The oil of larch strobilus from natural habitat was characterized by manool and 3-α-acetoxy-manool, diterpenes that were not present in the oil of cultivated sample. PB - Institute of Botany and Botanical Garden Jevremovac, Belgrade C3 - Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts T1 - Anatomy and composition of the essential oil of Larix decidua L. (Pinaceae) VL - 42 IS - 1 supplement SP - 129 EP - 130 UR - https://hdl.handle.net/21.15107/rcub_farfar_5254 ER -
@conference{ author = "Lakušić, Branislava and Marčetić, Mirjana", year = "2018", abstract = "Larix decidua L. is a deciduous coniferous native to the mountains of central Europe, in the Alps and Carpathians. The aim was to investigate anatomy and the chemical composition of the essential oils of needles, stems and strobilus of L. decidua. The samples were collected from larch trees from cultivated conditions (Belgrade, Serbia.) and from natural habitat (Alps, Austria). Needles of L. decidua, on cross section are more or less elliptically shaped. Two small secretory canals are in the mesophyll of the needles, on their lateral sides, just below the epidermis. The young stem (primary structures) have canals in cortex, some are just below the epidermis and many, that are in forming phase above the phloem. The older stem (perennial stem) has many secretory canals in the parenchyma of cortex and in the xylem of secondary wood. The essential oils were obtained by hydrodistillation and qualitative and quantitative analysis was performed by GC-FID and GC-MS. Small amounts of essential oils were extracted from the needles (0.06-0.41%), stems (0.31-0.54%) and strobilus (0.16 % and 0.19 %) of L. decidua. The monoterpene (28.70-50.40%) and sesquiterpene compounds (41.80-67.20%) dominated in the essential oil of needles, monoterpenes (80.50-91.70%) in the oil of stems, diterpenes (46.50% and 40.50%) and monoterpenes (24.20% and 22.20%) in the oil of strobilus. Main com- pounds in the essential oil of needles represented germacrene D (15.2-46.9%), δ-3-carene (4.6-24.6%) and α-pinene (6.3- 14.1%), in the oil of stems α-pinene (12.8-21.8%), δ-3-carene (8.6-14.3%) and sabinene (0.5-10.3%), while in the oil of strobilus diterpene of abietane type (4.1-10.2%) and nor-abietatetraene (3.9-9.2%) were the most abundant. The larch needles and stem essential oils from cultivated and natural habitats were quite similar in qualitative composition. However, the essential oil of needles from natural habitat contained higher content of germacrene D and α-pinene and the oil of stem α-pinene and trans-verbenol. The oil of larch strobilus from natural habitat was characterized by manool and 3-α-acetoxy-manool, diterpenes that were not present in the oil of cultivated sample.", publisher = "Institute of Botany and Botanical Garden Jevremovac, Belgrade", journal = "Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts", title = "Anatomy and composition of the essential oil of Larix decidua L. (Pinaceae)", volume = "42", number = "1 supplement", pages = "129-130", url = "https://hdl.handle.net/21.15107/rcub_farfar_5254" }
Lakušić, B.,& Marčetić, M.. (2018). Anatomy and composition of the essential oil of Larix decidua L. (Pinaceae). in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts Institute of Botany and Botanical Garden Jevremovac, Belgrade., 42(1 supplement), 129-130. https://hdl.handle.net/21.15107/rcub_farfar_5254
Lakušić B, Marčetić M. Anatomy and composition of the essential oil of Larix decidua L. (Pinaceae). in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts. 2018;42(1 supplement):129-130. https://hdl.handle.net/21.15107/rcub_farfar_5254 .
Lakušić, Branislava, Marčetić, Mirjana, "Anatomy and composition of the essential oil of Larix decidua L. (Pinaceae)" in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts, 42, no. 1 supplement (2018):129-130, https://hdl.handle.net/21.15107/rcub_farfar_5254 .