Controlling of the parthenolide partition using micelle structures of block copolymer in ionic liquid based aqueous biphasic system
Samo za registrovane korisnike
2019
Autori
Dimitrijević, AleksandraJocić, Ana
Marić, Slađana
Zdolšek, Nikola
Trtić-Petrović, Tatjana
Vraneš, Milan
Gadžurić, Slobodan
Arsenijević, Jelena
Ražić, Slavica
Tavares, Ana Paula M.
Freire, Mara G.
Konferencijski prilog (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The chemotherapeutic parthenolide (PTL) has an excellent
potential in the treatment of acute lymphoblastic leukemia and
much attention has been focused to overcome its poor aqueous
solubility, which limits PTL bioavailability. The most promising
approach is using micellar structures of Pluronics (class of triblock
copolymer of poly(ethylene)-poly(propylene)-poly(ethylene)),
as a drug delivery vectors. Pluronic PE6200 (PL6200) is selected
as one of the phase formers of aqueous biphasic system (ABS)
having in mind the amphiphilic nature of Pluronics which enables
good solubilization of hydrophobic molecules, their biocompatibility,
water solubility and complex mesophase behaviour. ABSs
are formed by combination of aqueous solutions of two polymers,
two salts or polymer/salt above certain concentrations with water
as the main constituent of both phases of ABS. In the quest for
more selective, performant and “greener” extraction technique of
PTL from natural matrices, a nove...l ABS comprised of a hydrophilic
ionic liquid (IL) - cholinium pyruvate (ChPyr) and PL6200
was investigated. In order to design selective Pluronic-mediated
purification process, (1) the ternary phase diagram was determinated
and characterised and (2) the partition of hydrophobic
PTL in this system was evaluated. A solution composed of 20
wt% of ChPyr + 30 wt% of PL6200 + 50 wt% of water with PTL
concentration of 2 mgml-1 led to high PTL extraction efficiency in
PL6200-rich phase (above 82%) with partition coefficient of 4.96.
The partition of PTL is mainly driven by its hydrophobic interaction
with poloxamer. For lower PL6200 concentrations in ABS,
formation of micelle particles was observed, what enabled solubilization
of PTL and its migration to hydrophilic IL-rich phase.
That decreased the PTL extraction efficiency toward PL6200-
rich phase. The obtained results showed that using cholinium-IL
/ Pluronic based ABS is promising alternative to volatile organic solvents that are nowdays used for extraction of PTL. Moreover,
using the Pluronic as constituent of ABS can provide the possibility
to simultaneously deal with low aqueous solubility of PTL by
forming the drug-loaded micelle particles and selective extraction
of active compound from real matrix.
Ključne reči:
Parthenolide / aqueous biphasic system / pluronicIzvor:
XX Euroanalysis 2019 Conference, Abstracts and Proceedings, 2019, 212-214Izdavač:
- Turkish Chemical Society
Finansiranje / projekti:
- Fizika i hemija sa jonskim snopovima (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45006)
- Project ID Project Title info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172012/RS// (RS-MESTD-Basic Research (BR or ON)-172012)
- Nove tehnologije za monitoring i zaštitu životnog okruženja od štetnih hemijskih supstanci i radijacionog opterećenja (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43009)
- Ispitivanje lekovitog potencijala biljaka: morfološka, hemijska i farmakološka karakterizacija (RS-MESTD-Basic Research (BR or ON)-173021)
- FCT Investigator Programme (IF/01634/2015)
Napomena:
- EuroAnalysis 2019, 20th European Conference on Analytical Chemistry, 1-5.9.2019, Istanbul, Turkey. Saopštenje sa međunarodnog skupa štampano u celini (kategorija M33)
Institucija/grupa
PharmacyTY - CONF AU - Dimitrijević, Aleksandra AU - Jocić, Ana AU - Marić, Slađana AU - Zdolšek, Nikola AU - Trtić-Petrović, Tatjana AU - Vraneš, Milan AU - Gadžurić, Slobodan AU - Arsenijević, Jelena AU - Ražić, Slavica AU - Tavares, Ana Paula M. AU - Freire, Mara G. PY - 2019 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5288 AB - The chemotherapeutic parthenolide (PTL) has an excellent potential in the treatment of acute lymphoblastic leukemia and much attention has been focused to overcome its poor aqueous solubility, which limits PTL bioavailability. The most promising approach is using micellar structures of Pluronics (class of triblock copolymer of poly(ethylene)-poly(propylene)-poly(ethylene)), as a drug delivery vectors. Pluronic PE6200 (PL6200) is selected as one of the phase formers of aqueous biphasic system (ABS) having in mind the amphiphilic nature of Pluronics which enables good solubilization of hydrophobic molecules, their biocompatibility, water solubility and complex mesophase behaviour. ABSs are formed by combination of aqueous solutions of two polymers, two salts or polymer/salt above certain concentrations with water as the main constituent of both phases of ABS. In the quest for more selective, performant and “greener” extraction technique of PTL from natural matrices, a novel ABS comprised of a hydrophilic ionic liquid (IL) - cholinium pyruvate (ChPyr) and PL6200 was investigated. In order to design selective Pluronic-mediated purification process, (1) the ternary phase diagram was determinated and characterised and (2) the partition of hydrophobic PTL in this system was evaluated. A solution composed of 20 wt% of ChPyr + 30 wt% of PL6200 + 50 wt% of water with PTL concentration of 2 mgml-1 led to high PTL extraction efficiency in PL6200-rich phase (above 82%) with partition coefficient of 4.96. The partition of PTL is mainly driven by its hydrophobic interaction with poloxamer. For lower PL6200 concentrations in ABS, formation of micelle particles was observed, what enabled solubilization of PTL and its migration to hydrophilic IL-rich phase. That decreased the PTL extraction efficiency toward PL6200- rich phase. The obtained results showed that using cholinium-IL / Pluronic based ABS is promising alternative to volatile organic solvents that are nowdays used for extraction of PTL. Moreover, using the Pluronic as constituent of ABS can provide the possibility to simultaneously deal with low aqueous solubility of PTL by forming the drug-loaded micelle particles and selective extraction of active compound from real matrix. PB - Turkish Chemical Society C3 - XX Euroanalysis 2019 Conference, Abstracts and Proceedings T1 - Controlling of the parthenolide partition using micelle structures of block copolymer in ionic liquid based aqueous biphasic system SP - 212 EP - 214 UR - https://hdl.handle.net/21.15107/rcub_farfar_5288 ER -
@conference{ author = "Dimitrijević, Aleksandra and Jocić, Ana and Marić, Slađana and Zdolšek, Nikola and Trtić-Petrović, Tatjana and Vraneš, Milan and Gadžurić, Slobodan and Arsenijević, Jelena and Ražić, Slavica and Tavares, Ana Paula M. and Freire, Mara G.", year = "2019", abstract = "The chemotherapeutic parthenolide (PTL) has an excellent potential in the treatment of acute lymphoblastic leukemia and much attention has been focused to overcome its poor aqueous solubility, which limits PTL bioavailability. The most promising approach is using micellar structures of Pluronics (class of triblock copolymer of poly(ethylene)-poly(propylene)-poly(ethylene)), as a drug delivery vectors. Pluronic PE6200 (PL6200) is selected as one of the phase formers of aqueous biphasic system (ABS) having in mind the amphiphilic nature of Pluronics which enables good solubilization of hydrophobic molecules, their biocompatibility, water solubility and complex mesophase behaviour. ABSs are formed by combination of aqueous solutions of two polymers, two salts or polymer/salt above certain concentrations with water as the main constituent of both phases of ABS. In the quest for more selective, performant and “greener” extraction technique of PTL from natural matrices, a novel ABS comprised of a hydrophilic ionic liquid (IL) - cholinium pyruvate (ChPyr) and PL6200 was investigated. In order to design selective Pluronic-mediated purification process, (1) the ternary phase diagram was determinated and characterised and (2) the partition of hydrophobic PTL in this system was evaluated. A solution composed of 20 wt% of ChPyr + 30 wt% of PL6200 + 50 wt% of water with PTL concentration of 2 mgml-1 led to high PTL extraction efficiency in PL6200-rich phase (above 82%) with partition coefficient of 4.96. The partition of PTL is mainly driven by its hydrophobic interaction with poloxamer. For lower PL6200 concentrations in ABS, formation of micelle particles was observed, what enabled solubilization of PTL and its migration to hydrophilic IL-rich phase. That decreased the PTL extraction efficiency toward PL6200- rich phase. The obtained results showed that using cholinium-IL / Pluronic based ABS is promising alternative to volatile organic solvents that are nowdays used for extraction of PTL. Moreover, using the Pluronic as constituent of ABS can provide the possibility to simultaneously deal with low aqueous solubility of PTL by forming the drug-loaded micelle particles and selective extraction of active compound from real matrix.", publisher = "Turkish Chemical Society", journal = "XX Euroanalysis 2019 Conference, Abstracts and Proceedings", title = "Controlling of the parthenolide partition using micelle structures of block copolymer in ionic liquid based aqueous biphasic system", pages = "212-214", url = "https://hdl.handle.net/21.15107/rcub_farfar_5288" }
Dimitrijević, A., Jocić, A., Marić, S., Zdolšek, N., Trtić-Petrović, T., Vraneš, M., Gadžurić, S., Arsenijević, J., Ražić, S., Tavares, A. P. M.,& Freire, M. G.. (2019). Controlling of the parthenolide partition using micelle structures of block copolymer in ionic liquid based aqueous biphasic system. in XX Euroanalysis 2019 Conference, Abstracts and Proceedings Turkish Chemical Society., 212-214. https://hdl.handle.net/21.15107/rcub_farfar_5288
Dimitrijević A, Jocić A, Marić S, Zdolšek N, Trtić-Petrović T, Vraneš M, Gadžurić S, Arsenijević J, Ražić S, Tavares APM, Freire MG. Controlling of the parthenolide partition using micelle structures of block copolymer in ionic liquid based aqueous biphasic system. in XX Euroanalysis 2019 Conference, Abstracts and Proceedings. 2019;:212-214. https://hdl.handle.net/21.15107/rcub_farfar_5288 .
Dimitrijević, Aleksandra, Jocić, Ana, Marić, Slađana, Zdolšek, Nikola, Trtić-Petrović, Tatjana, Vraneš, Milan, Gadžurić, Slobodan, Arsenijević, Jelena, Ražić, Slavica, Tavares, Ana Paula M., Freire, Mara G., "Controlling of the parthenolide partition using micelle structures of block copolymer in ionic liquid based aqueous biphasic system" in XX Euroanalysis 2019 Conference, Abstracts and Proceedings (2019):212-214, https://hdl.handle.net/21.15107/rcub_farfar_5288 .