Spectrophotometric determination of Bifonazole and Clotrimazole in lotions Bicutrin® and Canesten®

Abstract

A direct spectrophotometric method for the determination of bifonazole and clotrimazole in commercial lotions Bicutrin® and Canesten®, respectively, has been developed. The determinations were performed in a 0.1 M HCl solution at 257 nm (bifonazole) and 225 nm (clotrimazole). A calibration curves were obtained in the concentration range 1.5 -15.0 µg/ml and 2.5 - 25.0 µg/ml for bifonazole and clotrimazole, respectively, with correlation coefficient is 0.9999 for both curve. The accuracy and reproducibility of the method was checked by analyzing solutions of three different concentrations of bifonazole (recovery 98.7 - 99.7 % ; RSD 0.60-1.55 % ) and clotrimazole (recovery 98.3 - 99.1 % ; RSD 0.96 - 1.67 % ). The method proposed in this study is fast, accurate and reproductive. It enables the direct spectrophotometric determination of bifonazole and clotrimazole in lotions without previous isolation. The simplicity and low cost can be advantage of this method.

U radu je predložena direktna spektrofotometrijska metoda za određivanje antimikotika bifonazola i klotrimazola u losionima Bicutrin®, odnosno Canesten®. Određivanja su vršena u 0,1 M HCl na talasnoj dužini 257 nm (bifonazol), odnosno 225 nm (klotrimazol). Kalibraciona kriva dobijena je u opsegu koncentracija bifonazola 1,5-15 µg/ml, i klotrimazola 2,5-25 µg/ml, sa koeficientom korelacije 0,9999 za obe krive. Tačnost i reproduktivnost metode proverena je analizom rastvora tri različite koncentracije bifonazola („recovery” 98,7-99,7 % ; RSD 0,60-1,55 % ) i klotrimazola („recovery” 98,3-99,1 % ; RSD 0,96-1,67 % ). Dobijeni rezultati ukazuju na tačnost i reproduktivnost predložene spektrofotometrijske metode, kao i na mogućnost direknog spektrofotometrijskog određivanja bifonazola i klotrimazola u odgovarajućim losionima bez prethodnog izolovanja.