The current research provides a simplified sample preparation procedure for the accurate estimation of quercetin in pure and in the pharmaceutical dosage form. Direct spectrophotometric method for the determination of quercetin in the presence of ascorbic acid was established. The influences of medium, wavelength, pH, temperature and the ionic strengths on quercetin determination were investigated. The best conditions for calibration curve are: 50% ethanol, λ = 370 nm, pH = 4.2, T = 34°C and I = 7.5 × 10-5 M. Beer's law is obeyed in the concentration range 1.0-12.0 μg ml-1 for quercetin. The corresponding detection limit is 0.76 μg ml-1. The proposed method was verified by analyzing Quercetin + C capsules, Twinlab®.
Keywords:
Quercetin / Spectrophotometric method / Vitamin C
Source:
Farmaco, 2004, 59, 1, 21-24
Publisher:
Elsevier Masson SAS
Funding / projects:
Ministry of Sciences, Technologies and Development of the Republic of Serbia, grant No. 1458
TY - JOUR
AU - Pejić, Nataša
AU - Kuntić, Vesna
AU - Vujić, Zorica
AU - Micić, S
PY - 2004
UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/558
AB - The current research provides a simplified sample preparation procedure for the accurate estimation of quercetin in pure and in the pharmaceutical dosage form. Direct spectrophotometric method for the determination of quercetin in the presence of ascorbic acid was established. The influences of medium, wavelength, pH, temperature and the ionic strengths on quercetin determination were investigated. The best conditions for calibration curve are: 50% ethanol, λ = 370 nm, pH = 4.2, T = 34°C and I = 7.5 × 10-5 M. Beer's law is obeyed in the concentration range 1.0-12.0 μg ml-1 for quercetin. The corresponding detection limit is 0.76 μg ml-1. The proposed method was verified by analyzing Quercetin + C capsules, Twinlab®.
PB - Elsevier Masson SAS
T2 - Farmaco
T1 - Direct spectrophotometric determination of quercetin in the presence of ascorbic acid
VL - 59
IS - 1
SP - 21
EP - 24
DO - 10.1016/j.farmac.2003.07.013
ER -
@article{
author = "Pejić, Nataša and Kuntić, Vesna and Vujić, Zorica and Micić, S",
year = "2004",
abstract = "The current research provides a simplified sample preparation procedure for the accurate estimation of quercetin in pure and in the pharmaceutical dosage form. Direct spectrophotometric method for the determination of quercetin in the presence of ascorbic acid was established. The influences of medium, wavelength, pH, temperature and the ionic strengths on quercetin determination were investigated. The best conditions for calibration curve are: 50% ethanol, λ = 370 nm, pH = 4.2, T = 34°C and I = 7.5 × 10-5 M. Beer's law is obeyed in the concentration range 1.0-12.0 μg ml-1 for quercetin. The corresponding detection limit is 0.76 μg ml-1. The proposed method was verified by analyzing Quercetin + C capsules, Twinlab®.",
publisher = "Elsevier Masson SAS",
journal = "Farmaco",
title = "Direct spectrophotometric determination of quercetin in the presence of ascorbic acid",
volume = "59",
number = "1",
pages = "21-24",
doi = "10.1016/j.farmac.2003.07.013"
}
Pejić, N., Kuntić, V., Vujić, Z.,& Micić, S.. (2004). Direct spectrophotometric determination of quercetin in the presence of ascorbic acid. in Farmaco
Elsevier Masson SAS., 59(1), 21-24.
https://doi.org/10.1016/j.farmac.2003.07.013
Pejić N, Kuntić V, Vujić Z, Micić S. Direct spectrophotometric determination of quercetin in the presence of ascorbic acid. in Farmaco. 2004;59(1):21-24.
doi:10.1016/j.farmac.2003.07.013 .
Pejić, Nataša, Kuntić, Vesna, Vujić, Zorica, Micić, S, "Direct spectrophotometric determination of quercetin in the presence of ascorbic acid" in Farmaco, 59, no. 1 (2004):21-24,
https://doi.org/10.1016/j.farmac.2003.07.013 . .
