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Efekti različitih inhibitora azot oksid sintaze na oštećenje neurona indukovano hinolinskom kiselinom kod pacova

dc.creatorVasiljević, Ivana
dc.creatorJovanović, Marina
dc.creatorČolić, Miodrag
dc.creatorMihajlović, Rosa
dc.creatorĐukić, Mirjana
dc.creatorNinković, Milica
dc.creatorMaličević, Živorad
dc.date.accessioned2019-09-02T11:00:12Z
dc.date.available2019-09-02T11:00:12Z
dc.date.issued2004
dc.identifier.issn0354-3447
dc.identifier.urihttps://farfar.pharmacy.bg.ac.rs/handle/123456789/562
dc.description.abstractThe aetiology of neuronal death in neurodegenerative diseases, including Huntington-s disease, is still unknown. There could be a complex interplay among altered energy metabolism, excitotoxicity and oxidative stress. Our aim was to examine the effects of intrastriatal injection of a selective inhibitor of neuronal nitric oxide synthase, 7-nitroindazole, and a non-specific potent nitric oxide synthase inhibitor, Nw-nitro-L-arginine methyl ester, in order to study the possible involvement of glutathione, an important antioxidant, in quinolinic acid-induced striatal toxicity in the rat. Unilateral administration of quinolinic acid to rat striatum in a single dose of 150 nmol/L was used as a model of Huntington-s disease. The other group of animals were pretreated with 7- nitroindazole and Nw-nitro-L-arginine methyl ester, respectively. Control groups were treated with saline solution and olive oil, respectively. Content of total glutathione, was increased in the ipsi- and contralateral striatum, forebrain cortex, basal forebrain and hippocampus in the groups treated with nitric oxid synthase inhibitors and quinolinic acid compared to the quinolinic acid-treated animals. These results support the hypothesis that oxygen free radicals contribute to excitotoxic neuronal injury, and also that nitric oxide synthase inhibitors could be potential neuroprotective agents in Huntington-s disease.en
dc.description.abstractEtiologija selektivnog umiranja neurona u neurodegenerativnim bolestima je nepoznata, iako postoje dokazi o defektu energetskog metabolizma, ekscitotoksičnosti i oksidativnom oštećenju. Verovatno je da ključnu ulogu ima kompleksna interakcija između ovih mehanizama. Cilj ovog rada bio je da se ispitaju efekti intrastrijatne primene selektivnog inhibitora neuronske azot oksid sintaze, 7-nitroindazola, kao i nespecifičnog inhibitora azot oksid sintaze, Nw-nitro-l-arginin metil estra, zbog moguće uključenosti glutationa, ključnog antioksidansa, u toksičnost strijatuma izazvanu hinolinskom kiselinom, kod pacova. Unilateralna aplikacija hinolinske kiseline, u strijatum pacova u pojedinačnoj dozi od 150 nmol/L korišćena je kao model Hantingtonove bolesti. Druge grupe životinja tretirane su 7-nitroindazolom, odnosno Nw-nitro-l-arginin metil estrom. Kontrolne grupe dobijale su fiziološki rastvor, odnosno maslinovo ulje. Sadržaj ukupnog glutationa je povećan u ipsi- i kontralateralnom strijatumu, kori prednjeg mozga, bazalnom prednjem mozgu i hipokampusu grupa životinja koje su pored hinolinske kiseline primile i odgovarajući inhibitor neuronske azot oksid sintaze, u poređenju sa grupom tretiranom samo neurotoksinom. Ovi podaci pokazuju da kiseonični slobodni radikali učestvuju u ekscitotoksičnom oštećenju neurona, kao i da inhibitori azot oksid sintaze mogu biti potencijalni neuroprotektivni agensi u Hantingtonovoj bolesti.sr
dc.publisherDruštvo medicinskih biohemičara Srbije i Crne Gore, Beograd i Univerzitet u Beogradu - Farmaceutski fakultet, Beograd
dc.rightsopenAccess
dc.sourceJugoslovenska medicinska biohemija
dc.subjectHuntington-s diseaseen
dc.subjectquinolinic aciden
dc.subjectNw-nitro-L-arginine methyl esteren
dc.subject7-nitroindazoleen
dc.subjectglutathioneen
dc.subjectHantingtonova bolestsr
dc.subjecthinolinska kiselinasr
dc.subjectNw-nitro-l-arginin metil estarsr
dc.subject7-nitroindazolsr
dc.subjectglutationsr
dc.titleEffects of various nitric oxide synthase inhibitors on quinolinic acid-induced neuronal injury in ratsen
dc.titleEfekti različitih inhibitora azot oksid sintaze na oštećenje neurona indukovano hinolinskom kiselinom kod pacovasr
dc.typearticle
dc.rights.licenseARR
dcterms.abstractЧолић, Миодраг; Нинковић, Милица; Михајловић, Роса; Васиљевић, Ивана; Маличевић, Живорад; Ђукић, Мирјана; Јовановић, Марина; Ефекти различитих инхибитора азот оксид синтазе на оштећење неурона индуковано хинолинском киселином код пацова; Ефекти различитих инхибитора азот оксид синтазе на оштећење неурона индуковано хинолинском киселином код пацова;
dc.citation.volume23
dc.citation.issue1
dc.citation.spage11
dc.citation.epage18
dc.citation.other23(1): 11-18
dc.citation.rankM23
dc.identifier.doi10.2298/JMH0401011V
dc.identifier.scopus2-s2.0-1842427441
dc.identifier.fulltexthttps://farfar.pharmacy.bg.ac.rs//bitstream/id/2076/560.pdf
dc.identifier.rcubconv_831
dc.type.versionpublishedVersion


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