Use of pralidoxime-Pd(II) complex for the spectrophotometric determination of the cholinesterase reactivator (PAM-2Cl) in the urine
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A simple and rapid method for the spectrophotometric determination of pralidoxime chloride in urine, based on complex formation with palladium(II) without preliminary mineralization of the sample, is described. The proposed method is shown to be reproducible and in good agreement with a reference method, which involved spectrophotometric determination of corresponding oximate ions in ammonium hydroxide solution at 336 nm. Our results indicate that the proposed method is reliable, rapid and of sufficient sensitivity for pralidoxime chloride analysis in urine.