Development of liquid chromatographic method for fosinoprilat determination in human plasma using microemulsion as eluent
Samo za registrovane korisnike
2005
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Fosinopril sodium presents a prodrug for the active angiotensin converting enzyme (ACE) inhibitor, fosinoprilat. The dual elimination of fosinoprilat by the liver and the kidney distinguishes fosinopril from other angiotensin converting enzyme inhibitors. Such ways of elimination are important for antihypertensive therapy of patients on haemodialysis. The paper presents development and evaluation of a new and sensitive liquid chromatographic (LC) method for the analysis of fosinoprilat in plasma obtained from patients on haemodialysis. A microemulsion system mixture as mobile phase has been used for the separation and analysis of fosinoprilat in plasma samples. The plasma samples were injected directly onto the HPLC system (Waters Breeze) after appropriate sample dilution with mobile phase. Separations were performed on the Bakerbond ENV 4.6 mm x 150 mm, 5 mu m particle size column with UV detection at 220 nm. The flow rate was 1.00 mL min(-1). The mobile phase consisted of 1.0% (w/v) ...of diisopropyl ether, 2.0% (w/v) of sodium dodecyl sulphate (SDS), 6.0% (w/v) of n-propanol and 91 % (w/v) of aqueous 25 mM di-sodium hydrogen phosphate, pH adjusted to 2.8 with 85% orthophosphoric acid. The developed method was then subjected to method validation according to the criteria stated in the FDA bioanalytical method validation guidance. The results for specificity, linearity, low limit of quantification (LLOQ), precision, accuracy and stability were within the accepted criteria. The unique approach applied in this paper makes possible the determination of fosinoprilat even in the presence of metabolites of other drugs, so the method can be used for obtaining the reliable results in a fast and simple way.
Ključne reči:
fosinoprilat / liquid chromatography / human plasmaIzvor:
Journal of Chromatography A, 2005, 1088, 1-2, 187-192Izdavač:
- Elsevier Science BV, Amsterdam
DOI: 10.1016/j.chroma.2005.05.038
ISSN: 0021-9673
PubMed: 16130750
WoS: 000231185900024
Scopus: 2-s2.0-23044447454
Institucija/grupa
PharmacyTY - JOUR AU - Jančić, Biljana AU - Ivanović, D AU - Medenica, Mirjana AU - Malenović, Anđelija AU - Dimković, Nada PY - 2005 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/572 AB - Fosinopril sodium presents a prodrug for the active angiotensin converting enzyme (ACE) inhibitor, fosinoprilat. The dual elimination of fosinoprilat by the liver and the kidney distinguishes fosinopril from other angiotensin converting enzyme inhibitors. Such ways of elimination are important for antihypertensive therapy of patients on haemodialysis. The paper presents development and evaluation of a new and sensitive liquid chromatographic (LC) method for the analysis of fosinoprilat in plasma obtained from patients on haemodialysis. A microemulsion system mixture as mobile phase has been used for the separation and analysis of fosinoprilat in plasma samples. The plasma samples were injected directly onto the HPLC system (Waters Breeze) after appropriate sample dilution with mobile phase. Separations were performed on the Bakerbond ENV 4.6 mm x 150 mm, 5 mu m particle size column with UV detection at 220 nm. The flow rate was 1.00 mL min(-1). The mobile phase consisted of 1.0% (w/v) of diisopropyl ether, 2.0% (w/v) of sodium dodecyl sulphate (SDS), 6.0% (w/v) of n-propanol and 91 % (w/v) of aqueous 25 mM di-sodium hydrogen phosphate, pH adjusted to 2.8 with 85% orthophosphoric acid. The developed method was then subjected to method validation according to the criteria stated in the FDA bioanalytical method validation guidance. The results for specificity, linearity, low limit of quantification (LLOQ), precision, accuracy and stability were within the accepted criteria. The unique approach applied in this paper makes possible the determination of fosinoprilat even in the presence of metabolites of other drugs, so the method can be used for obtaining the reliable results in a fast and simple way. PB - Elsevier Science BV, Amsterdam T2 - Journal of Chromatography A T1 - Development of liquid chromatographic method for fosinoprilat determination in human plasma using microemulsion as eluent VL - 1088 IS - 1-2 SP - 187 EP - 192 DO - 10.1016/j.chroma.2005.05.038 ER -
@article{ author = "Jančić, Biljana and Ivanović, D and Medenica, Mirjana and Malenović, Anđelija and Dimković, Nada", year = "2005", abstract = "Fosinopril sodium presents a prodrug for the active angiotensin converting enzyme (ACE) inhibitor, fosinoprilat. The dual elimination of fosinoprilat by the liver and the kidney distinguishes fosinopril from other angiotensin converting enzyme inhibitors. Such ways of elimination are important for antihypertensive therapy of patients on haemodialysis. The paper presents development and evaluation of a new and sensitive liquid chromatographic (LC) method for the analysis of fosinoprilat in plasma obtained from patients on haemodialysis. A microemulsion system mixture as mobile phase has been used for the separation and analysis of fosinoprilat in plasma samples. The plasma samples were injected directly onto the HPLC system (Waters Breeze) after appropriate sample dilution with mobile phase. Separations were performed on the Bakerbond ENV 4.6 mm x 150 mm, 5 mu m particle size column with UV detection at 220 nm. The flow rate was 1.00 mL min(-1). The mobile phase consisted of 1.0% (w/v) of diisopropyl ether, 2.0% (w/v) of sodium dodecyl sulphate (SDS), 6.0% (w/v) of n-propanol and 91 % (w/v) of aqueous 25 mM di-sodium hydrogen phosphate, pH adjusted to 2.8 with 85% orthophosphoric acid. The developed method was then subjected to method validation according to the criteria stated in the FDA bioanalytical method validation guidance. The results for specificity, linearity, low limit of quantification (LLOQ), precision, accuracy and stability were within the accepted criteria. The unique approach applied in this paper makes possible the determination of fosinoprilat even in the presence of metabolites of other drugs, so the method can be used for obtaining the reliable results in a fast and simple way.", publisher = "Elsevier Science BV, Amsterdam", journal = "Journal of Chromatography A", title = "Development of liquid chromatographic method for fosinoprilat determination in human plasma using microemulsion as eluent", volume = "1088", number = "1-2", pages = "187-192", doi = "10.1016/j.chroma.2005.05.038" }
Jančić, B., Ivanović, D., Medenica, M., Malenović, A.,& Dimković, N.. (2005). Development of liquid chromatographic method for fosinoprilat determination in human plasma using microemulsion as eluent. in Journal of Chromatography A Elsevier Science BV, Amsterdam., 1088(1-2), 187-192. https://doi.org/10.1016/j.chroma.2005.05.038
Jančić B, Ivanović D, Medenica M, Malenović A, Dimković N. Development of liquid chromatographic method for fosinoprilat determination in human plasma using microemulsion as eluent. in Journal of Chromatography A. 2005;1088(1-2):187-192. doi:10.1016/j.chroma.2005.05.038 .
Jančić, Biljana, Ivanović, D, Medenica, Mirjana, Malenović, Anđelija, Dimković, Nada, "Development of liquid chromatographic method for fosinoprilat determination in human plasma using microemulsion as eluent" in Journal of Chromatography A, 1088, no. 1-2 (2005):187-192, https://doi.org/10.1016/j.chroma.2005.05.038 . .