A HPTLC method for the separation and identification of pentoxifylline and related substances, impurities of reaction partners, and side reaction products has been developed using different mobile and stationary phases. For quantitative assay of possible by-products as impurities, LiChrosphere (R) RP-18 F254s chromatoplates, acetone-chloroform-toluene-dioxane (221:11 v/v) as a mobile phase, and detection at 275 nm were employed. Linearity (r >= 0.997), recovery (86.5-115.5%), and determination limit (0.1-0.6%) were evaluated and found to be satisfactory. This method enables monitoring of the synthesis, as well as purity control of pentoxifylline-containing raw materials and pharmaceuticals.
TY - JOUR
AU - Grozdanović, Olivera
AU - Antić, Dušan
AU - Agbaba, Danica
PY - 2005
UR - http://farfar.pharmacy.bg.ac.rs/handle/123456789/587
AB - A HPTLC method for the separation and identification of pentoxifylline and related substances, impurities of reaction partners, and side reaction products has been developed using different mobile and stationary phases. For quantitative assay of possible by-products as impurities, LiChrosphere (R) RP-18 F254s chromatoplates, acetone-chloroform-toluene-dioxane (221:11 v/v) as a mobile phase, and detection at 275 nm were employed. Linearity (r >= 0.997), recovery (86.5-115.5%), and determination limit (0.1-0.6%) were evaluated and found to be satisfactory. This method enables monitoring of the synthesis, as well as purity control of pentoxifylline-containing raw materials and pharmaceuticals.
PB - Wiley-VCH Verlag GMBH, Weinheim
T2 - Journal of Separation Science
T1 - Development of a HPTLC method for in-process purity testing of pentoxifylline
VL - 28
IS - 6
SP - 575
EP - 580
DO - 10.1002/jssc.200400006
ER -
@article{
author = "Grozdanović, Olivera and Antić, Dušan and Agbaba, Danica",
year = "2005",
url = "http://farfar.pharmacy.bg.ac.rs/handle/123456789/587",
abstract = "A HPTLC method for the separation and identification of pentoxifylline and related substances, impurities of reaction partners, and side reaction products has been developed using different mobile and stationary phases. For quantitative assay of possible by-products as impurities, LiChrosphere (R) RP-18 F254s chromatoplates, acetone-chloroform-toluene-dioxane (221:11 v/v) as a mobile phase, and detection at 275 nm were employed. Linearity (r >= 0.997), recovery (86.5-115.5%), and determination limit (0.1-0.6%) were evaluated and found to be satisfactory. This method enables monitoring of the synthesis, as well as purity control of pentoxifylline-containing raw materials and pharmaceuticals.",
publisher = "Wiley-VCH Verlag GMBH, Weinheim",
journal = "Journal of Separation Science",
title = "Development of a HPTLC method for in-process purity testing of pentoxifylline",
volume = "28",
number = "6",
pages = "575-580",
doi = "10.1002/jssc.200400006"
}
Grozdanović, O., Antić, D.,& Agbaba, D. (2005). Development of a HPTLC method for in-process purity testing of pentoxifylline.
Journal of Separation Science
Wiley-VCH Verlag GMBH, Weinheim., 28(6), 575-580.
https://doi.org/10.1002/jssc.200400006
Grozdanović O, Antić D, Agbaba D. Development of a HPTLC method for in-process purity testing of pentoxifylline. Journal of Separation Science. 2005;28(6):575-580
Grozdanović Olivera, Antić Dušan, Agbaba Danica, "Development of a HPTLC method for in-process purity testing of pentoxifylline" Journal of Separation Science, 28, no. 6 (2005):575-580,
https://doi.org/10.1002/jssc.200400006 .
