A simple, precise and sensitive reverse-phase high performance liquid chromatographic (RP-HPLC) method has been developed for the quantitation of bifonazole, an imidazole antifungal, simultaneously with benzyl alcohol, used as preservative, in pharmaceutical formulations. Method employed Zorbax Eclipse XDB-C18 (250x4.6 mm i.d., 5 mu m) column, methanol-ammonium acetate (pH 2; 65 mM) (65:35, v/v, pH(*) 3.6) as mobile phase with flow rate of 1 mL min(-1) and variable UV detection at 220 and 252 nm. The proposed method was validated by testing its linearity, selectivity, recovery, repeatability, LOD/LOQ values and it was successfully employed for the determination of bifonazole and benzyl alcohol in pharmaceutical cream-based formulations.
TY - JOUR
AU - Čudina, Olivera
AU - Comor, Mirjana
AU - Janković, IA
PY - 2005
UR - http://farfar.pharmacy.bg.ac.rs/handle/123456789/588
AB - A simple, precise and sensitive reverse-phase high performance liquid chromatographic (RP-HPLC) method has been developed for the quantitation of bifonazole, an imidazole antifungal, simultaneously with benzyl alcohol, used as preservative, in pharmaceutical formulations. Method employed Zorbax Eclipse XDB-C18 (250x4.6 mm i.d., 5 mu m) column, methanol-ammonium acetate (pH 2; 65 mM) (65:35, v/v, pH(*) 3.6) as mobile phase with flow rate of 1 mL min(-1) and variable UV detection at 220 and 252 nm. The proposed method was validated by testing its linearity, selectivity, recovery, repeatability, LOD/LOQ values and it was successfully employed for the determination of bifonazole and benzyl alcohol in pharmaceutical cream-based formulations.
PB - Springer Heidelberg, Heidelberg
T2 - Chromatographia
T1 - Simultaneous determination of bifonazole and benzyl alcohol in pharmaceutical formulations by reverse-phase HPLC
VL - 61
IS - 7-8
SP - 415
EP - 418
DO - 10.1365/s10337-005-0524-9
UR - conv_1614
ER -
@article{
author = "Čudina, Olivera and Comor, Mirjana and Janković, IA",
year = "2005",
abstract = "A simple, precise and sensitive reverse-phase high performance liquid chromatographic (RP-HPLC) method has been developed for the quantitation of bifonazole, an imidazole antifungal, simultaneously with benzyl alcohol, used as preservative, in pharmaceutical formulations. Method employed Zorbax Eclipse XDB-C18 (250x4.6 mm i.d., 5 mu m) column, methanol-ammonium acetate (pH 2; 65 mM) (65:35, v/v, pH(*) 3.6) as mobile phase with flow rate of 1 mL min(-1) and variable UV detection at 220 and 252 nm. The proposed method was validated by testing its linearity, selectivity, recovery, repeatability, LOD/LOQ values and it was successfully employed for the determination of bifonazole and benzyl alcohol in pharmaceutical cream-based formulations.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Chromatographia",
title = "Simultaneous determination of bifonazole and benzyl alcohol in pharmaceutical formulations by reverse-phase HPLC",
volume = "61",
number = "7-8",
pages = "415-418",
doi = "10.1365/s10337-005-0524-9",
url = "conv_1614"
}
Čudina, O., Comor, M.,& Janković, I.. (2005). Simultaneous determination of bifonazole and benzyl alcohol in pharmaceutical formulations by reverse-phase HPLC. in Chromatographia
Springer Heidelberg, Heidelberg., 61(7-8), 415-418.
https://doi.org/10.1365/s10337-005-0524-9
conv_1614
Čudina O, Comor M, Janković I. Simultaneous determination of bifonazole and benzyl alcohol in pharmaceutical formulations by reverse-phase HPLC. in Chromatographia. 2005;61(7-8):415-418.
doi:10.1365/s10337-005-0524-9
conv_1614 .
Čudina, Olivera, Comor, Mirjana, Janković, IA, "Simultaneous determination of bifonazole and benzyl alcohol in pharmaceutical formulations by reverse-phase HPLC" in Chromatographia, 61, no. 7-8 (2005):415-418,
https://doi.org/10.1365/s10337-005-0524-9 .,
conv_1614 .
