In vitro cultures of plants from the rhamnaceae: Shoot propagation and anthraquinones production
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This study was conducted to investigate the effect of physical and chemical features on metabolic production in plant cell culture. The best multiplication of Frangula alnus Mill. shoots (8.84 +/- 1.11) was obtained on woody plant medium ( WPM) medium with indole-3-acetic acid (IAA) (0.1 mg l(-1)) and 6-benzylaminapurine (BAP) (1.0 mg l(-1)), but the highest metabolite production (1731 mg/100 g of total anthraquinone (AQ) aglycones) was in the shoots grown on the MS medium with addition of 1-naphthilaceneacetic (NAA) (0.1 mg l(-1)) and thidiazuron (TDZ) (0.1 mg l(-1)). The best multiplication of Frangula rupestris (Scop.) Schur. shoots (6.20 +/- 0.53) was obtained on MS medium supplemented with BAP (0.5 mg l(-1)). The highest metabolite production (451 mg/100 g of total anthraquinone aglycones) was in the shoots grown on medium with 2,4-D (0.1 mg l(-1)) and BAP (0.5 mg l(-1)). For the multiplication of Rhamnus catharticus L. shoots, the best results (2.8 +/- 0.7) were obtained on the m...edium with combination of 2,4-D (0.1 mg l(-1)) and kinetine (Kin) (1.0 mg l(-1)). The best synthesis of anthraquinones (AQs) (1205 mg/100 g of total AQ aglycones) was in the shoots, which were grown on MS medium supplemented with 2,4-D (0.1 mg l(-1)).
Keywords:anthraquinones production / Frangula alnus / Frangula rupestris / propagation / Rhamnus catharticus / shoot cultures
Source:Pharmaceutical Biology, 2005, 43, 5, 420-424
- Taylor & Francis Ltd, Abingdon