A reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed for separation of carvedilol and its impurities from Karvileks tablets. The best separation was achieved on a 100 mm x 4.6 mm, 5 mu m particle size, Chromolit RP 8e column. Use of acetonitrile-water, 45:55 (v/v), adjusted to pH 2.5 with formic acid, as mobile phase at a flow rate of 0.5 mL min(-1) enabled acceptable resolution of carvedilol, in large excess, from possible impurities, in a short elution time. UV detection was performed at 280 nm. Linearity, accuracy, precision, selectivity, and robustness were validated and found to be satisfactory. Overall, the proposed method was found to be highly sensitive, suitable, and accurate for quantitative determination of carvedilol and its impurities in dosage forms and in raw materials.
TY - JOUR
AU - Stojanović, J
AU - Marinković, Valentina
AU - Vladimirov, S
AU - Veličković, D.
AU - Sibinović, Predrag
PY - 2005
UR - http://farfar.pharmacy.bg.ac.rs/handle/123456789/623
AB - A reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed for separation of carvedilol and its impurities from Karvileks tablets. The best separation was achieved on a 100 mm x 4.6 mm, 5 mu m particle size, Chromolit RP 8e column. Use of acetonitrile-water, 45:55 (v/v), adjusted to pH 2.5 with formic acid, as mobile phase at a flow rate of 0.5 mL min(-1) enabled acceptable resolution of carvedilol, in large excess, from possible impurities, in a short elution time. UV detection was performed at 280 nm. Linearity, accuracy, precision, selectivity, and robustness were validated and found to be satisfactory. Overall, the proposed method was found to be highly sensitive, suitable, and accurate for quantitative determination of carvedilol and its impurities in dosage forms and in raw materials.
PB - Vieweg, Wiesbaden
T2 - Chromatographia
T1 - Determination of carvedilol and its impurities in pharmaceuticals
VL - 62
IS - 9-10
SP - 539
EP - 542
DO - 10.1365/s10337-005-0656-y
ER -
@article{
author = "Stojanović, J and Marinković, Valentina and Vladimirov, S and Veličković, D. and Sibinović, Predrag",
year = "2005",
url = "http://farfar.pharmacy.bg.ac.rs/handle/123456789/623",
abstract = "A reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed for separation of carvedilol and its impurities from Karvileks tablets. The best separation was achieved on a 100 mm x 4.6 mm, 5 mu m particle size, Chromolit RP 8e column. Use of acetonitrile-water, 45:55 (v/v), adjusted to pH 2.5 with formic acid, as mobile phase at a flow rate of 0.5 mL min(-1) enabled acceptable resolution of carvedilol, in large excess, from possible impurities, in a short elution time. UV detection was performed at 280 nm. Linearity, accuracy, precision, selectivity, and robustness were validated and found to be satisfactory. Overall, the proposed method was found to be highly sensitive, suitable, and accurate for quantitative determination of carvedilol and its impurities in dosage forms and in raw materials.",
publisher = "Vieweg, Wiesbaden",
journal = "Chromatographia",
title = "Determination of carvedilol and its impurities in pharmaceuticals",
volume = "62",
number = "9-10",
pages = "539-542",
doi = "10.1365/s10337-005-0656-y"
}
Stojanović J, Marinković V, Vladimirov S, Veličković D, Sibinović P. Determination of carvedilol and its impurities in pharmaceuticals. Chromatographia. 2005;62(9-10):539-542
Stojanović, J., Marinković, V., Vladimirov, S., Veličković, D.,& Sibinović, P. (2005). Determination of carvedilol and its impurities in pharmaceuticals.
ChromatographiaVieweg, Wiesbaden., 62(9-10), 539-542.
https://doi.org/10.1365/s10337-005-0656-y
Stojanović J, Marinković Valentina, Vladimirov S, Veličković D., Sibinović Predrag, "Determination of carvedilol and its impurities in pharmaceuticals" 62, no. 9-10 (2005):539-542,
https://doi.org/10.1365/s10337-005-0656-y .
