The simple and rapid RP-HPLC method, for the simultaneous determination of lidocaine and cetrimonium bromide in the presence of pellet color corrigent, was developed. Separations were performed on a Beckman Ultrasphere ODS 4.6 mm x 15 cm, 5 μm particle column at 40°C. The mobile phase consisted of water phase and acetonitrile (72:28:V/V), pH value of the mobile phase was adjusted to 2.0 with 85% ortophosphoric acid. Bisacodil was used as an internal standard. The flow rate was 1 ml/min and UV detection was performed at 208 nm. The proposed RP-HPLC method was validated and all the parameters for the validation of the method are given. According to the obtained results, the developed method was found to be suitable and accurate for the determination of these drugs in commercial formulations.
TY - JOUR
AU - Malenović, Anđelija
AU - Medenica, Mirjana
AU - Ivanović, D
AU - Jančić, Biljana
AU - Marković, S
PY - 2005
UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/649
AB - The simple and rapid RP-HPLC method, for the simultaneous determination of lidocaine and cetrimonium bromide in the presence of pellet color corrigent, was developed. Separations were performed on a Beckman Ultrasphere ODS 4.6 mm x 15 cm, 5 μm particle column at 40°C. The mobile phase consisted of water phase and acetonitrile (72:28:V/V), pH value of the mobile phase was adjusted to 2.0 with 85% ortophosphoric acid. Bisacodil was used as an internal standard. The flow rate was 1 ml/min and UV detection was performed at 208 nm. The proposed RP-HPLC method was validated and all the parameters for the validation of the method are given. According to the obtained results, the developed method was found to be suitable and accurate for the determination of these drugs in commercial formulations.
PB - Elsevier Masson SAS
T2 - Farmaco
T1 - Development and validation of RP-HPLC method for cetrimonium bromide and lidocaine determination
VL - 60
IS - 2
SP - 157
EP - 161
DO - 10.1016/j.farmac.2004.11.004
ER -
@article{
author = "Malenović, Anđelija and Medenica, Mirjana and Ivanović, D and Jančić, Biljana and Marković, S",
year = "2005",
abstract = "The simple and rapid RP-HPLC method, for the simultaneous determination of lidocaine and cetrimonium bromide in the presence of pellet color corrigent, was developed. Separations were performed on a Beckman Ultrasphere ODS 4.6 mm x 15 cm, 5 μm particle column at 40°C. The mobile phase consisted of water phase and acetonitrile (72:28:V/V), pH value of the mobile phase was adjusted to 2.0 with 85% ortophosphoric acid. Bisacodil was used as an internal standard. The flow rate was 1 ml/min and UV detection was performed at 208 nm. The proposed RP-HPLC method was validated and all the parameters for the validation of the method are given. According to the obtained results, the developed method was found to be suitable and accurate for the determination of these drugs in commercial formulations.",
publisher = "Elsevier Masson SAS",
journal = "Farmaco",
title = "Development and validation of RP-HPLC method for cetrimonium bromide and lidocaine determination",
volume = "60",
number = "2",
pages = "157-161",
doi = "10.1016/j.farmac.2004.11.004"
}
Malenović, A., Medenica, M., Ivanović, D., Jančić, B.,& Marković, S.. (2005). Development and validation of RP-HPLC method for cetrimonium bromide and lidocaine determination. in Farmaco
Elsevier Masson SAS., 60(2), 157-161.
https://doi.org/10.1016/j.farmac.2004.11.004
Malenović A, Medenica M, Ivanović D, Jančić B, Marković S. Development and validation of RP-HPLC method for cetrimonium bromide and lidocaine determination. in Farmaco. 2005;60(2):157-161.
doi:10.1016/j.farmac.2004.11.004 .
Malenović, Anđelija, Medenica, Mirjana, Ivanović, D, Jančić, Biljana, Marković, S, "Development and validation of RP-HPLC method for cetrimonium bromide and lidocaine determination" in Farmaco, 60, no. 2 (2005):157-161,
https://doi.org/10.1016/j.farmac.2004.11.004 . .
