Immunomodulatory effects of the methanolic extract of Epimedium alpinum in vitro
Само за регистроване кориснике
2006
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The effect of the methanolic extract of root and rhizome of Epimedium alpinum (MEEA) on phenotype and functions of rat lymphocytes in vitro was studied. It has been found that MEEA at lower concentrations (0.1 mu g/ml and 1 mu g/ml) significantly enhanced proliferation of splenocytes and thymocytes triggered by concanavalin A (Con A), whereas higher concentrations of the extract (50 mu g/ ml-500 mu g/ml) were inhibitory. The stimulatory effect of MEEA on Con A-induced proliferation of splenocytes correlated with the up-regulation of interleukin-2 receptor alpha (IL-2R alpha) expression. In addition, increased production of IL-2 was observed when a blocking IL-2R alpha monoclonal antibody (mAb) was added to cell cultures. MEEA-suppressed proliferation of splenocytes was due to the inhibition of IL-2 production, the down-regulation of IL-2Ra expression and the induction of apoptosis. Cellular proliferation in the presence of inhibitory concentrations of MEEA higher than 50 mu g/ml could ...not be restored by the addition of exogenous IL-2.
Кључне речи:
Epimedium alpinum / rat lymphocytes / IL-2 production / IL-2R alpha expression / apoptosisИзвор:
Fitoterapia, 2006, 77, 7-8, 561-567Издавач:
- Elsevier Science BV, Amsterdam
DOI: 10.1016/j.fitote.2006.09.008
ISSN: 0367-326X
PubMed: 17070658
WoS: 000242945900015
Scopus: 2-s2.0-33750945984
Институција/група
PharmacyTY - JOUR AU - Kovačević, Nada AU - Čolić, Miodrag AU - Backović, Aleksandar AU - Došlov-Kokoruš, Zvezdana PY - 2006 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/674 AB - The effect of the methanolic extract of root and rhizome of Epimedium alpinum (MEEA) on phenotype and functions of rat lymphocytes in vitro was studied. It has been found that MEEA at lower concentrations (0.1 mu g/ml and 1 mu g/ml) significantly enhanced proliferation of splenocytes and thymocytes triggered by concanavalin A (Con A), whereas higher concentrations of the extract (50 mu g/ ml-500 mu g/ml) were inhibitory. The stimulatory effect of MEEA on Con A-induced proliferation of splenocytes correlated with the up-regulation of interleukin-2 receptor alpha (IL-2R alpha) expression. In addition, increased production of IL-2 was observed when a blocking IL-2R alpha monoclonal antibody (mAb) was added to cell cultures. MEEA-suppressed proliferation of splenocytes was due to the inhibition of IL-2 production, the down-regulation of IL-2Ra expression and the induction of apoptosis. Cellular proliferation in the presence of inhibitory concentrations of MEEA higher than 50 mu g/ml could not be restored by the addition of exogenous IL-2. PB - Elsevier Science BV, Amsterdam T2 - Fitoterapia T1 - Immunomodulatory effects of the methanolic extract of Epimedium alpinum in vitro VL - 77 IS - 7-8 SP - 561 EP - 567 DO - 10.1016/j.fitote.2006.09.008 ER -
@article{ author = "Kovačević, Nada and Čolić, Miodrag and Backović, Aleksandar and Došlov-Kokoruš, Zvezdana", year = "2006", abstract = "The effect of the methanolic extract of root and rhizome of Epimedium alpinum (MEEA) on phenotype and functions of rat lymphocytes in vitro was studied. It has been found that MEEA at lower concentrations (0.1 mu g/ml and 1 mu g/ml) significantly enhanced proliferation of splenocytes and thymocytes triggered by concanavalin A (Con A), whereas higher concentrations of the extract (50 mu g/ ml-500 mu g/ml) were inhibitory. The stimulatory effect of MEEA on Con A-induced proliferation of splenocytes correlated with the up-regulation of interleukin-2 receptor alpha (IL-2R alpha) expression. In addition, increased production of IL-2 was observed when a blocking IL-2R alpha monoclonal antibody (mAb) was added to cell cultures. MEEA-suppressed proliferation of splenocytes was due to the inhibition of IL-2 production, the down-regulation of IL-2Ra expression and the induction of apoptosis. Cellular proliferation in the presence of inhibitory concentrations of MEEA higher than 50 mu g/ml could not be restored by the addition of exogenous IL-2.", publisher = "Elsevier Science BV, Amsterdam", journal = "Fitoterapia", title = "Immunomodulatory effects of the methanolic extract of Epimedium alpinum in vitro", volume = "77", number = "7-8", pages = "561-567", doi = "10.1016/j.fitote.2006.09.008" }
Kovačević, N., Čolić, M., Backović, A.,& Došlov-Kokoruš, Z.. (2006). Immunomodulatory effects of the methanolic extract of Epimedium alpinum in vitro. in Fitoterapia Elsevier Science BV, Amsterdam., 77(7-8), 561-567. https://doi.org/10.1016/j.fitote.2006.09.008
Kovačević N, Čolić M, Backović A, Došlov-Kokoruš Z. Immunomodulatory effects of the methanolic extract of Epimedium alpinum in vitro. in Fitoterapia. 2006;77(7-8):561-567. doi:10.1016/j.fitote.2006.09.008 .
Kovačević, Nada, Čolić, Miodrag, Backović, Aleksandar, Došlov-Kokoruš, Zvezdana, "Immunomodulatory effects of the methanolic extract of Epimedium alpinum in vitro" in Fitoterapia, 77, no. 7-8 (2006):561-567, https://doi.org/10.1016/j.fitote.2006.09.008 . .