The effect of the methanolic extract of root and rhizome of Epimedium alpinum (MEEA) on phenotype and functions of rat lymphocytes in vitro was studied. It has been found that MEEA at lower concentrations (0.1 mu g/ml and 1 mu g/ml) significantly enhanced proliferation of splenocytes and thymocytes triggered by concanavalin A (Con A), whereas higher concentrations of the extract (50 mu g/ ml-500 mu g/ml) were inhibitory. The stimulatory effect of MEEA on Con A-induced proliferation of splenocytes correlated with the up-regulation of interleukin-2 receptor alpha (IL-2R alpha) expression. In addition, increased production of IL-2 was observed when a blocking IL-2R alpha monoclonal antibody (mAb) was added to cell cultures. MEEA-suppressed proliferation of splenocytes was due to the inhibition of IL-2 production, the down-regulation of IL-2Ra expression and the induction of apoptosis. Cellular proliferation in the presence of inhibitory concentrations of MEEA higher than 50 mu g/ml could ...not be restored by the addition of exogenous IL-2.
Keywords:
Epimedium alpinum / rat lymphocytes / IL-2 production / IL-2R alpha expression / apoptosis
TY - JOUR
AU - Kovačević, Nada
AU - Čolić, Miodrag
AU - Backović, Aleksandar
AU - Došlov-Kokoruš, Zvezdana
PY - 2006
UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/674
AB - The effect of the methanolic extract of root and rhizome of Epimedium alpinum (MEEA) on phenotype and functions of rat lymphocytes in vitro was studied. It has been found that MEEA at lower concentrations (0.1 mu g/ml and 1 mu g/ml) significantly enhanced proliferation of splenocytes and thymocytes triggered by concanavalin A (Con A), whereas higher concentrations of the extract (50 mu g/ ml-500 mu g/ml) were inhibitory. The stimulatory effect of MEEA on Con A-induced proliferation of splenocytes correlated with the up-regulation of interleukin-2 receptor alpha (IL-2R alpha) expression. In addition, increased production of IL-2 was observed when a blocking IL-2R alpha monoclonal antibody (mAb) was added to cell cultures. MEEA-suppressed proliferation of splenocytes was due to the inhibition of IL-2 production, the down-regulation of IL-2Ra expression and the induction of apoptosis. Cellular proliferation in the presence of inhibitory concentrations of MEEA higher than 50 mu g/ml could not be restored by the addition of exogenous IL-2.
PB - Elsevier Science BV, Amsterdam
T2 - Fitoterapia
T1 - Immunomodulatory effects of the methanolic extract of Epimedium alpinum in vitro
VL - 77
IS - 7-8
SP - 561
EP - 567
DO - 10.1016/j.fitote.2006.09.008
ER -
@article{
author = "Kovačević, Nada and Čolić, Miodrag and Backović, Aleksandar and Došlov-Kokoruš, Zvezdana",
year = "2006",
abstract = "The effect of the methanolic extract of root and rhizome of Epimedium alpinum (MEEA) on phenotype and functions of rat lymphocytes in vitro was studied. It has been found that MEEA at lower concentrations (0.1 mu g/ml and 1 mu g/ml) significantly enhanced proliferation of splenocytes and thymocytes triggered by concanavalin A (Con A), whereas higher concentrations of the extract (50 mu g/ ml-500 mu g/ml) were inhibitory. The stimulatory effect of MEEA on Con A-induced proliferation of splenocytes correlated with the up-regulation of interleukin-2 receptor alpha (IL-2R alpha) expression. In addition, increased production of IL-2 was observed when a blocking IL-2R alpha monoclonal antibody (mAb) was added to cell cultures. MEEA-suppressed proliferation of splenocytes was due to the inhibition of IL-2 production, the down-regulation of IL-2Ra expression and the induction of apoptosis. Cellular proliferation in the presence of inhibitory concentrations of MEEA higher than 50 mu g/ml could not be restored by the addition of exogenous IL-2.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Fitoterapia",
title = "Immunomodulatory effects of the methanolic extract of Epimedium alpinum in vitro",
volume = "77",
number = "7-8",
pages = "561-567",
doi = "10.1016/j.fitote.2006.09.008"
}
Kovačević, N., Čolić, M., Backović, A.,& Došlov-Kokoruš, Z.. (2006). Immunomodulatory effects of the methanolic extract of Epimedium alpinum in vitro. in Fitoterapia
Elsevier Science BV, Amsterdam., 77(7-8), 561-567.
https://doi.org/10.1016/j.fitote.2006.09.008
Kovačević N, Čolić M, Backović A, Došlov-Kokoruš Z. Immunomodulatory effects of the methanolic extract of Epimedium alpinum in vitro. in Fitoterapia. 2006;77(7-8):561-567.
doi:10.1016/j.fitote.2006.09.008 .
Kovačević, Nada, Čolić, Miodrag, Backović, Aleksandar, Došlov-Kokoruš, Zvezdana, "Immunomodulatory effects of the methanolic extract of Epimedium alpinum in vitro" in Fitoterapia, 77, no. 7-8 (2006):561-567,
https://doi.org/10.1016/j.fitote.2006.09.008 . .
