Premature centromere division (PCD) of the chromosome 18 was analyzed by using fluorescent in situ hybridization (FISH) on interphase peripheral blood lymphocytes isolated from six sporadic Alzheimer disease (AD) patients and six healthy elderly controls. Results of FISH analysis revealed that chromosome 18 expressed PCD in 5.18% interphase nuclei of AD patients, and in 2.59% interphase nuclei of age-matched controls (p lt 0.05). Our study also showed that hypoploidy and hyperploidy frequency for chromosome 18 exhibited a statistically significant increase in the AD group compared to the control one. The increase in spontaneous aneuploidy of chromosome 18 in AD patients which is correlated with PCD shows that deregulation of the time of centromere separation can be considered as a manifestation of chromosome instability leading to aneuploidy.
TY - JOUR
AU - Živković, Lada
AU - Potparević, Biljana
AU - Đejić, Ninoslav
AU - Bajić, Vladan
PY - 2006
UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/677
AB - Premature centromere division (PCD) of the chromosome 18 was analyzed by using fluorescent in situ hybridization (FISH) on interphase peripheral blood lymphocytes isolated from six sporadic Alzheimer disease (AD) patients and six healthy elderly controls. Results of FISH analysis revealed that chromosome 18 expressed PCD in 5.18% interphase nuclei of AD patients, and in 2.59% interphase nuclei of age-matched controls (p lt 0.05). Our study also showed that hypoploidy and hyperploidy frequency for chromosome 18 exhibited a statistically significant increase in the AD group compared to the control one. The increase in spontaneous aneuploidy of chromosome 18 in AD patients which is correlated with PCD shows that deregulation of the time of centromere separation can be considered as a manifestation of chromosome instability leading to aneuploidy.
PB - Elsevier Ireland Ltd, Clare
T2 - Mechanisms of Ageing and Development
T1 - Analysis of premature centromere division (PCD) of the chromosome 18 in peripheral blood lymphocytes in Alzheimer disease patients
VL - 127
IS - 12
SP - 892
EP - 896
DO - 10.1016/j.mad.2006.09.004
ER -
@article{
author = "Živković, Lada and Potparević, Biljana and Đejić, Ninoslav and Bajić, Vladan",
year = "2006",
abstract = "Premature centromere division (PCD) of the chromosome 18 was analyzed by using fluorescent in situ hybridization (FISH) on interphase peripheral blood lymphocytes isolated from six sporadic Alzheimer disease (AD) patients and six healthy elderly controls. Results of FISH analysis revealed that chromosome 18 expressed PCD in 5.18% interphase nuclei of AD patients, and in 2.59% interphase nuclei of age-matched controls (p lt 0.05). Our study also showed that hypoploidy and hyperploidy frequency for chromosome 18 exhibited a statistically significant increase in the AD group compared to the control one. The increase in spontaneous aneuploidy of chromosome 18 in AD patients which is correlated with PCD shows that deregulation of the time of centromere separation can be considered as a manifestation of chromosome instability leading to aneuploidy.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Mechanisms of Ageing and Development",
title = "Analysis of premature centromere division (PCD) of the chromosome 18 in peripheral blood lymphocytes in Alzheimer disease patients",
volume = "127",
number = "12",
pages = "892-896",
doi = "10.1016/j.mad.2006.09.004"
}
Živković, L., Potparević, B., Đejić, N.,& Bajić, V.. (2006). Analysis of premature centromere division (PCD) of the chromosome 18 in peripheral blood lymphocytes in Alzheimer disease patients. in Mechanisms of Ageing and Development
Elsevier Ireland Ltd, Clare., 127(12), 892-896.
https://doi.org/10.1016/j.mad.2006.09.004
Živković L, Potparević B, Đejić N, Bajić V. Analysis of premature centromere division (PCD) of the chromosome 18 in peripheral blood lymphocytes in Alzheimer disease patients. in Mechanisms of Ageing and Development. 2006;127(12):892-896.
doi:10.1016/j.mad.2006.09.004 .
Živković, Lada, Potparević, Biljana, Đejić, Ninoslav, Bajić, Vladan, "Analysis of premature centromere division (PCD) of the chromosome 18 in peripheral blood lymphocytes in Alzheimer disease patients" in Mechanisms of Ageing and Development, 127, no. 12 (2006):892-896,
https://doi.org/10.1016/j.mad.2006.09.004 . .
