Genetic transformation of Rhamnus fallax and hairy roots as a source of anthraquinones
Само за регистроване кориснике
2006
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Hairy roots of Rhamnus fallax Boiss. were induced using Agrobacterium rhizogenes strain A4M70GUS. The culture established on Woody plant media (WPM) showed a typical hairy root phenotype: rapid growth, reduced apical dominance and root plagiotropism. Seven clones of R. fallax were selected on the basis of their differences in colour and the root branching. The growth of hairy root culture, measured through gain in fresh mass, was done under 16-h photoperiod or in the dark. An increase in anthraquinone (AQ) content was obtained in clones with yellow and less branched roots, like clone 1 [16.43 mg g(-1)(d.m.)] and clone 7 [14.21 mg g(-1)(d.m.)], compared with other analysed transformed and non-transformed tissue. This study presents the first report of successful transformation of any species from family Rhamnaceae by A. rhizogenes and analysis of AQ production in transformed tissue.
Кључне речи:
Agrobacterium rhizogenes / transformed roots / secondary metabolitesИзвор:
Biologia Plantarum, 2006, 50, 4, 514-518Издавач:
- Springer, Dordrecht
DOI: 10.1007/s10535-006-0081-6
ISSN: 0006-3134
WoS: 000238531000005
Scopus: 2-s2.0-33745657248
Институција/група
PharmacyTY - JOUR AU - Rosić, Nedeljka AU - Momcilović, I. AU - Kovačević, Nada AU - Grubišić, Dragoljub PY - 2006 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/725 AB - Hairy roots of Rhamnus fallax Boiss. were induced using Agrobacterium rhizogenes strain A4M70GUS. The culture established on Woody plant media (WPM) showed a typical hairy root phenotype: rapid growth, reduced apical dominance and root plagiotropism. Seven clones of R. fallax were selected on the basis of their differences in colour and the root branching. The growth of hairy root culture, measured through gain in fresh mass, was done under 16-h photoperiod or in the dark. An increase in anthraquinone (AQ) content was obtained in clones with yellow and less branched roots, like clone 1 [16.43 mg g(-1)(d.m.)] and clone 7 [14.21 mg g(-1)(d.m.)], compared with other analysed transformed and non-transformed tissue. This study presents the first report of successful transformation of any species from family Rhamnaceae by A. rhizogenes and analysis of AQ production in transformed tissue. PB - Springer, Dordrecht T2 - Biologia Plantarum T1 - Genetic transformation of Rhamnus fallax and hairy roots as a source of anthraquinones VL - 50 IS - 4 SP - 514 EP - 518 DO - 10.1007/s10535-006-0081-6 ER -
@article{ author = "Rosić, Nedeljka and Momcilović, I. and Kovačević, Nada and Grubišić, Dragoljub", year = "2006", abstract = "Hairy roots of Rhamnus fallax Boiss. were induced using Agrobacterium rhizogenes strain A4M70GUS. The culture established on Woody plant media (WPM) showed a typical hairy root phenotype: rapid growth, reduced apical dominance and root plagiotropism. Seven clones of R. fallax were selected on the basis of their differences in colour and the root branching. The growth of hairy root culture, measured through gain in fresh mass, was done under 16-h photoperiod or in the dark. An increase in anthraquinone (AQ) content was obtained in clones with yellow and less branched roots, like clone 1 [16.43 mg g(-1)(d.m.)] and clone 7 [14.21 mg g(-1)(d.m.)], compared with other analysed transformed and non-transformed tissue. This study presents the first report of successful transformation of any species from family Rhamnaceae by A. rhizogenes and analysis of AQ production in transformed tissue.", publisher = "Springer, Dordrecht", journal = "Biologia Plantarum", title = "Genetic transformation of Rhamnus fallax and hairy roots as a source of anthraquinones", volume = "50", number = "4", pages = "514-518", doi = "10.1007/s10535-006-0081-6" }
Rosić, N., Momcilović, I., Kovačević, N.,& Grubišić, D.. (2006). Genetic transformation of Rhamnus fallax and hairy roots as a source of anthraquinones. in Biologia Plantarum Springer, Dordrecht., 50(4), 514-518. https://doi.org/10.1007/s10535-006-0081-6
Rosić N, Momcilović I, Kovačević N, Grubišić D. Genetic transformation of Rhamnus fallax and hairy roots as a source of anthraquinones. in Biologia Plantarum. 2006;50(4):514-518. doi:10.1007/s10535-006-0081-6 .
Rosić, Nedeljka, Momcilović, I., Kovačević, Nada, Grubišić, Dragoljub, "Genetic transformation of Rhamnus fallax and hairy roots as a source of anthraquinones" in Biologia Plantarum, 50, no. 4 (2006):514-518, https://doi.org/10.1007/s10535-006-0081-6 . .