Development and validation of liquid chromatography method for the separation of valdecoxib and its SC-77852 impurity
Само за регистроване кориснике
2006
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
A reversed-phase liquid chromatography method has been developed for the separation of valdecoxib and impurity SC-77852. The best results were achieved using a mobile phase-methanol: 1% water solution TEA (52 : 48 v/v), pH 7.35 (adjusted with 85% orthophosphoric acid), column temperature 24 degrees C. Separation was carried out on XTerra (TM) RP18 (150mm x 4,6 mm), particle size 5 mu m, flow rate 1ml/min, using detection on 220 nm. The method was statistically validated for its selectivity, linearity, precision (repeatability), and robustness. Quantitation and detection limits were determined for both valdecoxib and SC-77852. Method robustness was further evaluated by performing full factorial design experiment. Validated method was used for assay of valdecoxib and SC-77852 in Bextra (R) film-coated tablets.
Кључне речи:
valdecoxib / robustness / experimental design / RP HPLC / SC-77852Извор:
Analytical Letters, 2006, 39, 9, 1875-1890Издавач:
- Taylor & Francis Inc, Philadelphia
Финансирање / пројекти:
- Синтеза, квантитативни односи између структуре/особина и активности, физичко-хемијска карактеризација и анализа фармаколошки активних супстанци (RS-MESTD-MPN2006-2010-142071)
DOI: 10.1080/00032710600721605
ISSN: 0003-2719
WoS: 000238565900009
Scopus: 2-s2.0-33745626824
Институција/група
PharmacyTY - JOUR AU - Zečević, Mira AU - Savić, G AU - Živanović, L PY - 2006 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/775 AB - A reversed-phase liquid chromatography method has been developed for the separation of valdecoxib and impurity SC-77852. The best results were achieved using a mobile phase-methanol: 1% water solution TEA (52 : 48 v/v), pH 7.35 (adjusted with 85% orthophosphoric acid), column temperature 24 degrees C. Separation was carried out on XTerra (TM) RP18 (150mm x 4,6 mm), particle size 5 mu m, flow rate 1ml/min, using detection on 220 nm. The method was statistically validated for its selectivity, linearity, precision (repeatability), and robustness. Quantitation and detection limits were determined for both valdecoxib and SC-77852. Method robustness was further evaluated by performing full factorial design experiment. Validated method was used for assay of valdecoxib and SC-77852 in Bextra (R) film-coated tablets. PB - Taylor & Francis Inc, Philadelphia T2 - Analytical Letters T1 - Development and validation of liquid chromatography method for the separation of valdecoxib and its SC-77852 impurity VL - 39 IS - 9 SP - 1875 EP - 1890 DO - 10.1080/00032710600721605 ER -
@article{ author = "Zečević, Mira and Savić, G and Živanović, L", year = "2006", abstract = "A reversed-phase liquid chromatography method has been developed for the separation of valdecoxib and impurity SC-77852. The best results were achieved using a mobile phase-methanol: 1% water solution TEA (52 : 48 v/v), pH 7.35 (adjusted with 85% orthophosphoric acid), column temperature 24 degrees C. Separation was carried out on XTerra (TM) RP18 (150mm x 4,6 mm), particle size 5 mu m, flow rate 1ml/min, using detection on 220 nm. The method was statistically validated for its selectivity, linearity, precision (repeatability), and robustness. Quantitation and detection limits were determined for both valdecoxib and SC-77852. Method robustness was further evaluated by performing full factorial design experiment. Validated method was used for assay of valdecoxib and SC-77852 in Bextra (R) film-coated tablets.", publisher = "Taylor & Francis Inc, Philadelphia", journal = "Analytical Letters", title = "Development and validation of liquid chromatography method for the separation of valdecoxib and its SC-77852 impurity", volume = "39", number = "9", pages = "1875-1890", doi = "10.1080/00032710600721605" }
Zečević, M., Savić, G.,& Živanović, L.. (2006). Development and validation of liquid chromatography method for the separation of valdecoxib and its SC-77852 impurity. in Analytical Letters Taylor & Francis Inc, Philadelphia., 39(9), 1875-1890. https://doi.org/10.1080/00032710600721605
Zečević M, Savić G, Živanović L. Development and validation of liquid chromatography method for the separation of valdecoxib and its SC-77852 impurity. in Analytical Letters. 2006;39(9):1875-1890. doi:10.1080/00032710600721605 .
Zečević, Mira, Savić, G, Živanović, L, "Development and validation of liquid chromatography method for the separation of valdecoxib and its SC-77852 impurity" in Analytical Letters, 39, no. 9 (2006):1875-1890, https://doi.org/10.1080/00032710600721605 . .