A reversed-phase liquid chromatography method has been developed for the separation of valdecoxib and impurity SC-77852. The best results were achieved using a mobile phase-methanol: 1% water solution TEA (52 : 48 v/v), pH 7.35 (adjusted with 85% orthophosphoric acid), column temperature 24 degrees C. Separation was carried out on XTerra (TM) RP18 (150mm x 4,6 mm), particle size 5 mu m, flow rate 1ml/min, using detection on 220 nm. The method was statistically validated for its selectivity, linearity, precision (repeatability), and robustness. Quantitation and detection limits were determined for both valdecoxib and SC-77852. Method robustness was further evaluated by performing full factorial design experiment. Validated method was used for assay of valdecoxib and SC-77852 in Bextra (R) film-coated tablets.
TY - JOUR
AU - Zečević, Mira
AU - Savić, G
AU - Živanović, L
PY - 2006
UR - http://farfar.pharmacy.bg.ac.rs/handle/123456789/775
AB - A reversed-phase liquid chromatography method has been developed for the separation of valdecoxib and impurity SC-77852. The best results were achieved using a mobile phase-methanol: 1% water solution TEA (52 : 48 v/v), pH 7.35 (adjusted with 85% orthophosphoric acid), column temperature 24 degrees C. Separation was carried out on XTerra (TM) RP18 (150mm x 4,6 mm), particle size 5 mu m, flow rate 1ml/min, using detection on 220 nm. The method was statistically validated for its selectivity, linearity, precision (repeatability), and robustness. Quantitation and detection limits were determined for both valdecoxib and SC-77852. Method robustness was further evaluated by performing full factorial design experiment. Validated method was used for assay of valdecoxib and SC-77852 in Bextra (R) film-coated tablets.
PB - Taylor & Francis Inc, Philadelphia
T2 - Analytical Letters
T1 - Development and validation of liquid chromatography method for the separation of valdecoxib and its SC-77852 impurity
VL - 39
IS - 9
SP - 1875
EP - 1890
DO - 10.1080/00032710600721605
ER -
@article{
author = "Zečević, Mira and Savić, G and Živanović, L",
year = "2006",
url = "http://farfar.pharmacy.bg.ac.rs/handle/123456789/775",
abstract = "A reversed-phase liquid chromatography method has been developed for the separation of valdecoxib and impurity SC-77852. The best results were achieved using a mobile phase-methanol: 1% water solution TEA (52 : 48 v/v), pH 7.35 (adjusted with 85% orthophosphoric acid), column temperature 24 degrees C. Separation was carried out on XTerra (TM) RP18 (150mm x 4,6 mm), particle size 5 mu m, flow rate 1ml/min, using detection on 220 nm. The method was statistically validated for its selectivity, linearity, precision (repeatability), and robustness. Quantitation and detection limits were determined for both valdecoxib and SC-77852. Method robustness was further evaluated by performing full factorial design experiment. Validated method was used for assay of valdecoxib and SC-77852 in Bextra (R) film-coated tablets.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Analytical Letters",
title = "Development and validation of liquid chromatography method for the separation of valdecoxib and its SC-77852 impurity",
volume = "39",
number = "9",
pages = "1875-1890",
doi = "10.1080/00032710600721605"
}
Zečević, M., Savić, G.,& Živanović, L. (2006). Development and validation of liquid chromatography method for the separation of valdecoxib and its SC-77852 impurity.
Analytical Letters
Taylor & Francis Inc, Philadelphia., 39(9), 1875-1890.
https://doi.org/10.1080/00032710600721605
Zečević M, Savić G, Živanović L. Development and validation of liquid chromatography method for the separation of valdecoxib and its SC-77852 impurity. Analytical Letters. 2006;39(9):1875-1890
Zečević Mira, Savić G, Živanović L, "Development and validation of liquid chromatography method for the separation of valdecoxib and its SC-77852 impurity" Analytical Letters, 39, no. 9 (2006):1875-1890,
https://doi.org/10.1080/00032710600721605 .
