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Investigation of chromatographic conditions for the separation of ofloxacin and its degradation products

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2006
Authors
Živanović, Lj.
Zigić, G.
Zečević, Mira
Article (Published version)
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Abstract
A sensitive, precise, and accurate reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed and validated for simultaneous determination of fluoroquinolone antibacterial ofloxacin and its degradation products: decarboxy ofloxacin, 9-piperazino ofloxacin, des-methyl ofloxacin, and ofloxacin-N-oxide. The proposed RP-HPLC method allowed separation of all five compounds simultaneously with the total time of the analysis not more than 15 min. The relative standard deviation (RSD) values for quantification of DOF, POF, MOF, OF, and NOF were of 0.77, 0.58, 0.51, 0.10, and 0.70%, respectively, indicating a good precision of the method. The limits of detection for DOF, POF, MOF, OF, and NOF were 0.10, 0.13, 0.06, 0.03, and 0.03 mu g mL(-1), respectively. The described method can be used for simultaneous identification and quantification of all analysed compounds in different pharmaceutical formulations.
Keywords:
liquid chromatography / ofloxacin / degradation products / determination
Source:
Journal of Chromatography A, 2006, 1119, 1-2, 224-230
Publisher:
  • Elsevier Science BV, Amsterdam

DOI: 10.1016/j.chroma.2006.02.029

ISSN: 0021-9673

PubMed: 16551467

WoS: 000238707100031

Scopus: 2-s2.0-33744792287
[ Google Scholar ]
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URI
http://farfar.pharmacy.bg.ac.rs/handle/123456789/814
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  • Radovi istraživača / Researchers’ publications
Institution
Pharmacy

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