Age-associated changes in CD90 expression on thymocytes and in TCR-dependent stages of thymocyte maturation in male rats
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2006
Authors
Leposavić, Gordana
Pešić, Vesna

Kosec, Duško
Radojević, Katarina
Arsenović-Ranin, Nevena

Pilipović, Ivan
Perišić, Milica
Plećaš-Solarović, Bosiljka
Article (Published version)

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To elucidate the effects of ageing on T-cell-maturation, in 3- and 18-month-old rats, we analysed the expression of: (i) CD4/CD8/TCR alpha beta and (ii) Thy-1, which is supposed to be a regulator of TCR alpha beta signalling, and thereby the thymocyte selection thresholds. Since an essential role for TCR alpha beta signalling in the development of CD4+25+T-reg-cells was suggested, the frequency of these cells was also quantified. We demonstrated that, as for mice, early thymocyte differentiational steps within the CD4-8- double negative (DN) developmental stage are age-sensitive. Furthermore, we revealed that TCRao-dependent stages of T-cell development are affected by ageing, most likely due to an impaired expression of Thy-1 on TCR alpha beta(low) thymocytes entering selection processes. The diminished frequency of the post-selection CD4+8+ double positive (DP) cells in aged rats, together with an overrepresentation of mature single positive (SP) cells, most probably suggests more ef...ficient differentiational transition from the DP TCR alpha beta(high) to the SP TCR alpha beta(high) developmental stage, which is followed by an increase in pre-migration proliferation of the mature SP cells. Moreover, the study indicated impaired intrathymic generation of CD4+25+T-reg-cells in aged rats, thus providing a possible explanation for the increased frequency of autoimmune diseases in ageing.
Keywords:
ageing / T-cell differentiation / CD90 expression / thymocyte apoptosis / ConA / CD4+25+thymocytesSource:
Experimental Gerontology, 2006, 41, 6, 574-589Publisher:
- Pergamon-Elsevier Science Ltd, Oxford
Funding / projects:
DOI: 10.1016/j.exger.2006.03.006
ISSN: 0531-5565
PubMed: 16632291
WoS: 000238899600006
Scopus: 2-s2.0-33745000082
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PharmacyTY - JOUR AU - Leposavić, Gordana AU - Pešić, Vesna AU - Kosec, Duško AU - Radojević, Katarina AU - Arsenović-Ranin, Nevena AU - Pilipović, Ivan AU - Perišić, Milica AU - Plećaš-Solarović, Bosiljka PY - 2006 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/816 AB - To elucidate the effects of ageing on T-cell-maturation, in 3- and 18-month-old rats, we analysed the expression of: (i) CD4/CD8/TCR alpha beta and (ii) Thy-1, which is supposed to be a regulator of TCR alpha beta signalling, and thereby the thymocyte selection thresholds. Since an essential role for TCR alpha beta signalling in the development of CD4+25+T-reg-cells was suggested, the frequency of these cells was also quantified. We demonstrated that, as for mice, early thymocyte differentiational steps within the CD4-8- double negative (DN) developmental stage are age-sensitive. Furthermore, we revealed that TCRao-dependent stages of T-cell development are affected by ageing, most likely due to an impaired expression of Thy-1 on TCR alpha beta(low) thymocytes entering selection processes. The diminished frequency of the post-selection CD4+8+ double positive (DP) cells in aged rats, together with an overrepresentation of mature single positive (SP) cells, most probably suggests more efficient differentiational transition from the DP TCR alpha beta(high) to the SP TCR alpha beta(high) developmental stage, which is followed by an increase in pre-migration proliferation of the mature SP cells. Moreover, the study indicated impaired intrathymic generation of CD4+25+T-reg-cells in aged rats, thus providing a possible explanation for the increased frequency of autoimmune diseases in ageing. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Experimental Gerontology T1 - Age-associated changes in CD90 expression on thymocytes and in TCR-dependent stages of thymocyte maturation in male rats VL - 41 IS - 6 SP - 574 EP - 589 DO - 10.1016/j.exger.2006.03.006 ER -
@article{ author = "Leposavić, Gordana and Pešić, Vesna and Kosec, Duško and Radojević, Katarina and Arsenović-Ranin, Nevena and Pilipović, Ivan and Perišić, Milica and Plećaš-Solarović, Bosiljka", year = "2006", abstract = "To elucidate the effects of ageing on T-cell-maturation, in 3- and 18-month-old rats, we analysed the expression of: (i) CD4/CD8/TCR alpha beta and (ii) Thy-1, which is supposed to be a regulator of TCR alpha beta signalling, and thereby the thymocyte selection thresholds. Since an essential role for TCR alpha beta signalling in the development of CD4+25+T-reg-cells was suggested, the frequency of these cells was also quantified. We demonstrated that, as for mice, early thymocyte differentiational steps within the CD4-8- double negative (DN) developmental stage are age-sensitive. Furthermore, we revealed that TCRao-dependent stages of T-cell development are affected by ageing, most likely due to an impaired expression of Thy-1 on TCR alpha beta(low) thymocytes entering selection processes. The diminished frequency of the post-selection CD4+8+ double positive (DP) cells in aged rats, together with an overrepresentation of mature single positive (SP) cells, most probably suggests more efficient differentiational transition from the DP TCR alpha beta(high) to the SP TCR alpha beta(high) developmental stage, which is followed by an increase in pre-migration proliferation of the mature SP cells. Moreover, the study indicated impaired intrathymic generation of CD4+25+T-reg-cells in aged rats, thus providing a possible explanation for the increased frequency of autoimmune diseases in ageing.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Experimental Gerontology", title = "Age-associated changes in CD90 expression on thymocytes and in TCR-dependent stages of thymocyte maturation in male rats", volume = "41", number = "6", pages = "574-589", doi = "10.1016/j.exger.2006.03.006" }
Leposavić, G., Pešić, V., Kosec, D., Radojević, K., Arsenović-Ranin, N., Pilipović, I., Perišić, M.,& Plećaš-Solarović, B.. (2006). Age-associated changes in CD90 expression on thymocytes and in TCR-dependent stages of thymocyte maturation in male rats. in Experimental Gerontology Pergamon-Elsevier Science Ltd, Oxford., 41(6), 574-589. https://doi.org/10.1016/j.exger.2006.03.006
Leposavić G, Pešić V, Kosec D, Radojević K, Arsenović-Ranin N, Pilipović I, Perišić M, Plećaš-Solarović B. Age-associated changes in CD90 expression on thymocytes and in TCR-dependent stages of thymocyte maturation in male rats. in Experimental Gerontology. 2006;41(6):574-589. doi:10.1016/j.exger.2006.03.006 .
Leposavić, Gordana, Pešić, Vesna, Kosec, Duško, Radojević, Katarina, Arsenović-Ranin, Nevena, Pilipović, Ivan, Perišić, Milica, Plećaš-Solarović, Bosiljka, "Age-associated changes in CD90 expression on thymocytes and in TCR-dependent stages of thymocyte maturation in male rats" in Experimental Gerontology, 41, no. 6 (2006):574-589, https://doi.org/10.1016/j.exger.2006.03.006 . .