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Comparison of liquid chromatography with fluorescence detection to liquid chromatography-mass spectrometry for the determination of fluoxetine and norfluoxetine in human plasma

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2006
Authors
Kovacević, I
Miljković, Branislava
Jovanović, D
Prostran, Milica
Article (Published version)
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Abstract
A comparison study on fluoxetine (FL) and norfluoxetine (NORFL) quantitation in human plasma was carried out between the recently developed liquid chromatographic method with fluorescence detection (LC-FLD) and an earlier established liquid chromatography-mass spectrometry (LC-MS) laboratory procedure. Comparative method evaluation was based on the analysis of plasma samples obtained from Parkinsonian patients receiving 20 mg of FL per day. The LC-FLD method involves a two-step liquid extraction procedure without any derivatization, followed by direct chromatography on a Zorbax C8 reversed-phase column. The analytical results are discussed in terms of the method validation and the corresponding experimental protocol (r >= 0.998; CV lt 9%; LOQ 20 mu g/l). There was good correlation between FL, as well as NORFL, plasma levels as determined by the LC-MS and LC-FLD techniques (r = 0.9597, N = 16 and r = 0.9852, N = 14 for FL and NORFL, respectively). The results confirm that direct FL/NO...RFL fluorimetric determination is acceptable for routine use in pharmacokinetic and clinical studies.

Keywords:
fluoxetine / norfluoxetine / liquid chromatography / fluorescence detection / mass spectrometry / human plasma / pharmacokinetic analysis
Source:
Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2006, 830, 2, 372-376
Publisher:
  • Elsevier Science BV, Amsterdam

DOI: 10.1016/j.jchromb.2005.11.034

ISSN: 1570-0232

PubMed: 16356789

WoS: 000234864300029

Scopus: 2-s2.0-30444455896
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URI
http://farfar.pharmacy.bg.ac.rs/handle/123456789/845
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Pharmacy

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