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dc.creatorKovacević, I
dc.creatorMiljković, Branislava
dc.creatorJovanović, D
dc.creatorProstran, Milica
dc.date.accessioned2019-09-02T11:07:34Z
dc.date.available2019-09-02T11:07:34Z
dc.date.issued2006
dc.identifier.issn1570-0232
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/845
dc.description.abstractA comparison study on fluoxetine (FL) and norfluoxetine (NORFL) quantitation in human plasma was carried out between the recently developed liquid chromatographic method with fluorescence detection (LC-FLD) and an earlier established liquid chromatography-mass spectrometry (LC-MS) laboratory procedure. Comparative method evaluation was based on the analysis of plasma samples obtained from Parkinsonian patients receiving 20 mg of FL per day. The LC-FLD method involves a two-step liquid extraction procedure without any derivatization, followed by direct chromatography on a Zorbax C8 reversed-phase column. The analytical results are discussed in terms of the method validation and the corresponding experimental protocol (r >= 0.998; CV lt 9%; LOQ 20 mu g/l). There was good correlation between FL, as well as NORFL, plasma levels as determined by the LC-MS and LC-FLD techniques (r = 0.9597, N = 16 and r = 0.9852, N = 14 for FL and NORFL, respectively). The results confirm that direct FL/NORFL fluorimetric determination is acceptable for routine use in pharmacokinetic and clinical studies.en
dc.publisherElsevier Science BV, Amsterdam
dc.rightsrestrictedAccess
dc.sourceJournal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
dc.subjectfluoxetineen
dc.subjectnorfluoxetineen
dc.subjectliquid chromatographyen
dc.subjectfluorescence detectionen
dc.subjectmass spectrometryen
dc.subjecthuman plasmaen
dc.subjectpharmacokinetic analysisen
dc.titleComparison of liquid chromatography with fluorescence detection to liquid chromatography-mass spectrometry for the determination of fluoxetine and norfluoxetine in human plasmaen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractЈовановић, Д; Миљковић, Бранислава; Ковацевић, И; Простран, Милица;
dc.citation.volume830
dc.citation.issue2
dc.citation.spage372
dc.citation.epage376
dc.citation.other830(2): 372-376
dc.citation.rankM21
dc.identifier.wos000234864300029
dc.identifier.doi10.1016/j.jchromb.2005.11.034
dc.identifier.pmid16356789
dc.identifier.scopus2-s2.0-30444455896
dc.identifier.rcubconv_1688
dc.type.versionpublishedVersion


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