Sister chromatid exchange and micronuclei in human peripheral blood lymphocytes treated with thyroxine in vitro
Abstract
Thyroid hormones enhance the metabolic rate and the aerobic metabolism favoring oxidative stress, which is accompanied by induction of damage to cellular macromolecules including the DNA. The aim of the present study was to investigate the ability of thyroxine to induce sister chromatid exchange and micronuclei, and to modulate cell-cycle kinetics in cultured human lymphocytes. Eight experimental concentrations of thyroxine were used, ranging from 2 x 10(-9) to 0.5 x 10(-4) M. Treatment with thyroxine increased the frequency of SCE per cell at the higher concentrations (1.5 x 10(-6), 0.5 10(-5), 1.5 x 10(-5) and 0.5 x 10(-4) M). On the other hand, there were no significant aneugenic and/or clastogenic effects observed in the cytokinesis-block micronucleus assay. The results show that thyroxine acted as a relatively weak clastogen compared with the positive control N-methyl-N '-nitro-N-nitrosoguanidine (MNNG). In addition to the genotoxic effects, two high concentrations of thyroxine de...creased the mitotic index and caused cell-cycle delay. In conclusion, thyroxine exhibited weak clastogenic effects only at high concentrations. Therefore, effects in humans might appear in cases of acute thyroxine overdose.
Keywords:
thyroxine / sister chromatid exchange / micronuclei / lymphocytesSource:
Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 2006, 604, 1-2, 1-7Publisher:
- Elsevier Science BV, Amsterdam
Funding / projects:
DOI: 10.1016/j.mrgentox.2005.11.013
ISSN: 1383-5718
PubMed: 16513411
WoS: 000236994700001
Scopus: 2-s2.0-33645976774
Collections
Institution/Community
PharmacyTY - JOUR AU - Đelić, N AU - Potparević, Biljana AU - Bajić, Vladan AU - Đelić, D PY - 2006 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/851 AB - Thyroid hormones enhance the metabolic rate and the aerobic metabolism favoring oxidative stress, which is accompanied by induction of damage to cellular macromolecules including the DNA. The aim of the present study was to investigate the ability of thyroxine to induce sister chromatid exchange and micronuclei, and to modulate cell-cycle kinetics in cultured human lymphocytes. Eight experimental concentrations of thyroxine were used, ranging from 2 x 10(-9) to 0.5 x 10(-4) M. Treatment with thyroxine increased the frequency of SCE per cell at the higher concentrations (1.5 x 10(-6), 0.5 10(-5), 1.5 x 10(-5) and 0.5 x 10(-4) M). On the other hand, there were no significant aneugenic and/or clastogenic effects observed in the cytokinesis-block micronucleus assay. The results show that thyroxine acted as a relatively weak clastogen compared with the positive control N-methyl-N '-nitro-N-nitrosoguanidine (MNNG). In addition to the genotoxic effects, two high concentrations of thyroxine decreased the mitotic index and caused cell-cycle delay. In conclusion, thyroxine exhibited weak clastogenic effects only at high concentrations. Therefore, effects in humans might appear in cases of acute thyroxine overdose. PB - Elsevier Science BV, Amsterdam T2 - Mutation Research - Genetic Toxicology and Environmental Mutagenesis T1 - Sister chromatid exchange and micronuclei in human peripheral blood lymphocytes treated with thyroxine in vitro VL - 604 IS - 1-2 SP - 1 EP - 7 DO - 10.1016/j.mrgentox.2005.11.013 ER -
@article{ author = "Đelić, N and Potparević, Biljana and Bajić, Vladan and Đelić, D", year = "2006", abstract = "Thyroid hormones enhance the metabolic rate and the aerobic metabolism favoring oxidative stress, which is accompanied by induction of damage to cellular macromolecules including the DNA. The aim of the present study was to investigate the ability of thyroxine to induce sister chromatid exchange and micronuclei, and to modulate cell-cycle kinetics in cultured human lymphocytes. Eight experimental concentrations of thyroxine were used, ranging from 2 x 10(-9) to 0.5 x 10(-4) M. Treatment with thyroxine increased the frequency of SCE per cell at the higher concentrations (1.5 x 10(-6), 0.5 10(-5), 1.5 x 10(-5) and 0.5 x 10(-4) M). On the other hand, there were no significant aneugenic and/or clastogenic effects observed in the cytokinesis-block micronucleus assay. The results show that thyroxine acted as a relatively weak clastogen compared with the positive control N-methyl-N '-nitro-N-nitrosoguanidine (MNNG). In addition to the genotoxic effects, two high concentrations of thyroxine decreased the mitotic index and caused cell-cycle delay. In conclusion, thyroxine exhibited weak clastogenic effects only at high concentrations. Therefore, effects in humans might appear in cases of acute thyroxine overdose.", publisher = "Elsevier Science BV, Amsterdam", journal = "Mutation Research - Genetic Toxicology and Environmental Mutagenesis", title = "Sister chromatid exchange and micronuclei in human peripheral blood lymphocytes treated with thyroxine in vitro", volume = "604", number = "1-2", pages = "1-7", doi = "10.1016/j.mrgentox.2005.11.013" }
Đelić, N., Potparević, B., Bajić, V.,& Đelić, D.. (2006). Sister chromatid exchange and micronuclei in human peripheral blood lymphocytes treated with thyroxine in vitro. in Mutation Research - Genetic Toxicology and Environmental Mutagenesis Elsevier Science BV, Amsterdam., 604(1-2), 1-7. https://doi.org/10.1016/j.mrgentox.2005.11.013
Đelić N, Potparević B, Bajić V, Đelić D. Sister chromatid exchange and micronuclei in human peripheral blood lymphocytes treated with thyroxine in vitro. in Mutation Research - Genetic Toxicology and Environmental Mutagenesis. 2006;604(1-2):1-7. doi:10.1016/j.mrgentox.2005.11.013 .
Đelić, N, Potparević, Biljana, Bajić, Vladan, Đelić, D, "Sister chromatid exchange and micronuclei in human peripheral blood lymphocytes treated with thyroxine in vitro" in Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 604, no. 1-2 (2006):1-7, https://doi.org/10.1016/j.mrgentox.2005.11.013 . .