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Determination of ascorbic acid in pharmaceutical dosage forms and urine by means of an oscillatory reaction system using the pulse perturbation technique
A simple and reliable method for the determination of ascorbic acid (AA) is proposed and validated. It is based on potentiometric monitoring of the concentration perturbations of an oscillatory reaction system in a stable nonequilibrium stationary state close to the bifurcation point. The response of the Bray-Liebhafsky (BL) oscillatory reaction as a matrix, to the perturbation by different concentrations of AA, is followed by a Pt electrode. The linear relationship between maximal potential shift and the logarithm of the amount of AA is obtained between 0.01 and 1.0 mu mol. The sensitivity of the proposed method (as the limit of detection) is 0.009 mu mol and the method has excellent sample throughput (30 samples per hour). The procedure was used for AA determination in pharmaceutical formulations and urine. The results are in agreement with those obtained using the official method. Some aspects of the possible mechanism of AA action on the BL oscillating chemical system are discussed....
Keywords:
determination of ascorbic acid / perturbation technique / bray-liebhafsky oscillatory reaction / pharmaceutical dosage forms / urine
Source:
Analytical and Bioanalytical Chemistry, 2007, 389, 6, 2009-2017
TY - JOUR
AU - Pejić, Nataša
AU - Blagojević, Slavica
AU - Anić, Slobodan
AU - Kolar-Anić, Ljiljana
PY - 2007
UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/892
AB - A simple and reliable method for the determination of ascorbic acid (AA) is proposed and validated. It is based on potentiometric monitoring of the concentration perturbations of an oscillatory reaction system in a stable nonequilibrium stationary state close to the bifurcation point. The response of the Bray-Liebhafsky (BL) oscillatory reaction as a matrix, to the perturbation by different concentrations of AA, is followed by a Pt electrode. The linear relationship between maximal potential shift and the logarithm of the amount of AA is obtained between 0.01 and 1.0 mu mol. The sensitivity of the proposed method (as the limit of detection) is 0.009 mu mol and the method has excellent sample throughput (30 samples per hour). The procedure was used for AA determination in pharmaceutical formulations and urine. The results are in agreement with those obtained using the official method. Some aspects of the possible mechanism of AA action on the BL oscillating chemical system are discussed.
PB - Springer Heidelberg, Heidelberg
T2 - Analytical and Bioanalytical Chemistry
T1 - Determination of ascorbic acid in pharmaceutical dosage forms and urine by means of an oscillatory reaction system using the pulse perturbation technique
VL - 389
IS - 6
SP - 2009
EP - 2017
DO - 10.1007/s00216-007-1585-4
ER -
@article{
author = "Pejić, Nataša and Blagojević, Slavica and Anić, Slobodan and Kolar-Anić, Ljiljana",
year = "2007",
abstract = "A simple and reliable method for the determination of ascorbic acid (AA) is proposed and validated. It is based on potentiometric monitoring of the concentration perturbations of an oscillatory reaction system in a stable nonequilibrium stationary state close to the bifurcation point. The response of the Bray-Liebhafsky (BL) oscillatory reaction as a matrix, to the perturbation by different concentrations of AA, is followed by a Pt electrode. The linear relationship between maximal potential shift and the logarithm of the amount of AA is obtained between 0.01 and 1.0 mu mol. The sensitivity of the proposed method (as the limit of detection) is 0.009 mu mol and the method has excellent sample throughput (30 samples per hour). The procedure was used for AA determination in pharmaceutical formulations and urine. The results are in agreement with those obtained using the official method. Some aspects of the possible mechanism of AA action on the BL oscillating chemical system are discussed.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Analytical and Bioanalytical Chemistry",
title = "Determination of ascorbic acid in pharmaceutical dosage forms and urine by means of an oscillatory reaction system using the pulse perturbation technique",
volume = "389",
number = "6",
pages = "2009-2017",
doi = "10.1007/s00216-007-1585-4"
}
Pejić, N., Blagojević, S., Anić, S.,& Kolar-Anić, L.. (2007). Determination of ascorbic acid in pharmaceutical dosage forms and urine by means of an oscillatory reaction system using the pulse perturbation technique. in Analytical and Bioanalytical Chemistry
Springer Heidelberg, Heidelberg., 389(6), 2009-2017.
https://doi.org/10.1007/s00216-007-1585-4
Pejić N, Blagojević S, Anić S, Kolar-Anić L. Determination of ascorbic acid in pharmaceutical dosage forms and urine by means of an oscillatory reaction system using the pulse perturbation technique. in Analytical and Bioanalytical Chemistry. 2007;389(6):2009-2017.
doi:10.1007/s00216-007-1585-4 .
Pejić, Nataša, Blagojević, Slavica, Anić, Slobodan, Kolar-Anić, Ljiljana, "Determination of ascorbic acid in pharmaceutical dosage forms and urine by means of an oscillatory reaction system using the pulse perturbation technique" in Analytical and Bioanalytical Chemistry, 389, no. 6 (2007):2009-2017,
https://doi.org/10.1007/s00216-007-1585-4 . .
