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dc.creatorĐelić, Ninoslav
dc.creatorĐelić, Dijana J.
dc.creatorPotparević, Biljana
dc.creatorŽivković, Lada
dc.creatorMarković, Biljana
dc.creatorLozance, Olivera
dc.creatorBlagojević, Miloš
dc.date.accessioned2019-09-02T11:08:57Z
dc.date.available2019-09-02T11:08:57Z
dc.date.issued2007
dc.identifier.issn1415-4757
dc.identifier.urihttps://farfar.pharmacy.bg.ac.rs/handle/123456789/901
dc.description.abstractThyroid hormones stimulate aerobic metabolism which may lead to oxidative stress accompanied by damage to various cellular macromolecules, including DNA. Previous comet assay studies have shown that thyroid hormones cause DNA damage due to the creation of reactive oxygen species (ROS). However, cytogenetic studies have been equivocal because although an increase in the sister-chromatid exchange frequency per cell has been reported increased micronuclei frequency has not. We used cytogenetic examination of chromosome breakage and aberrations in whole-blood cultures of human peripheral blood lymphocytes to investigate possible clastogenic effects when lymphocytes were exposed to 0.002 mu M to 50 mu M of L-thyroxine for 24 h and 48 h, these concentrations being chosen because they had been used in previous studies of sister-chromatid exchange and micronuclei frequency. Under our experimental conditions thyroxine did not induced any statistically significant increase in chromosome breakage or aberrations. This lack of clastogenic effects is in contrast to the reported comet assay results obtained using purified lymphocytes, possibly because whole-blood cultures contain catalase and glutathione peroxidase capable of reducing the effects of reactive oxygen species.en
dc.publisherSoc Brasil Genetica, Ribeirao Pret
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/143018/RS//
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.sourceGenetics and Molecular Biology
dc.subjectthyroxineen
dc.subjecthuman lymphocytesen
dc.subjectchromosome aberrationsen
dc.subjectmitotic indexen
dc.subjectROSen
dc.titleLack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytesen
dc.typearticle
dc.rights.licenseBY-NC
dcterms.abstractПотпаревић, Биљана; Живковић, Лада; Благојевић, Милош; Лозанце, Оливера; Марковић, Биљана; Ђелић, Дијана Ј.; Ђелић, Нинослав;
dc.citation.volume30
dc.citation.issue4
dc.citation.spage1144
dc.citation.epage1149
dc.citation.other30(4): 1144-1149
dc.citation.rankM23
dc.identifier.wos000251771400019
dc.identifier.doi10.1590/S1415-47572007000600019
dc.identifier.scopus2-s2.0-37649000465
dc.identifier.fulltexthttps://farfar.pharmacy.bg.ac.rs//bitstream/id/2252/899.pdf
dc.type.versionpublishedVersion


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