Chromatographic determination of dissociation constants of pramipexole and its impurities
Abstract
The main goal in this investigation was monocratic HPLC determination of dissociation constant values (pK(a)) of pramipexole and its impurities, BI-II 546 CL, BI-II 751 xx and 2-aminobenzothiazole. The chromatographic method is advantageous as a small amount of substance is needed and the degree of substance purity is less important. Analysis was carried out using stationary phases stable in a wide pH range. Triethylamonium phosphoric buffer was selected as appropriate pH controlling solution because it can cover a wide pH range. Detection was done on two different wavelengths, 262 nm for pramipexole, BI-II 751 xx and 2-aminobenzothiazole and at 326 nm for BI-II 546 CL. The constants were calculated from the typical sigmoidal curves of analyte obtained as retention factor versus the pH of the mobile phase.
Keywords:
column liquid chromatography / dissociation constants / impurities / pramipexoleSource:
Chromatographia, 2007, 65, 9-10, 633-635Publisher:
- Vieweg, Wiesbaden
Funding / projects:
DOI: 10.1365/s10337-007-0199-5
ISSN: 0009-5893
WoS: 000246878900021
Scopus: 2-s2.0-34247844409
Collections
Institution/Community
PharmacyTY - JOUR AU - Jančić, Biljana AU - Medenica, Mirjana AU - Ivanović, D. AU - Malenović, Anđelija PY - 2007 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/920 AB - The main goal in this investigation was monocratic HPLC determination of dissociation constant values (pK(a)) of pramipexole and its impurities, BI-II 546 CL, BI-II 751 xx and 2-aminobenzothiazole. The chromatographic method is advantageous as a small amount of substance is needed and the degree of substance purity is less important. Analysis was carried out using stationary phases stable in a wide pH range. Triethylamonium phosphoric buffer was selected as appropriate pH controlling solution because it can cover a wide pH range. Detection was done on two different wavelengths, 262 nm for pramipexole, BI-II 751 xx and 2-aminobenzothiazole and at 326 nm for BI-II 546 CL. The constants were calculated from the typical sigmoidal curves of analyte obtained as retention factor versus the pH of the mobile phase. PB - Vieweg, Wiesbaden T2 - Chromatographia T1 - Chromatographic determination of dissociation constants of pramipexole and its impurities VL - 65 IS - 9-10 SP - 633 EP - 635 DO - 10.1365/s10337-007-0199-5 ER -
@article{ author = "Jančić, Biljana and Medenica, Mirjana and Ivanović, D. and Malenović, Anđelija", year = "2007", abstract = "The main goal in this investigation was monocratic HPLC determination of dissociation constant values (pK(a)) of pramipexole and its impurities, BI-II 546 CL, BI-II 751 xx and 2-aminobenzothiazole. The chromatographic method is advantageous as a small amount of substance is needed and the degree of substance purity is less important. Analysis was carried out using stationary phases stable in a wide pH range. Triethylamonium phosphoric buffer was selected as appropriate pH controlling solution because it can cover a wide pH range. Detection was done on two different wavelengths, 262 nm for pramipexole, BI-II 751 xx and 2-aminobenzothiazole and at 326 nm for BI-II 546 CL. The constants were calculated from the typical sigmoidal curves of analyte obtained as retention factor versus the pH of the mobile phase.", publisher = "Vieweg, Wiesbaden", journal = "Chromatographia", title = "Chromatographic determination of dissociation constants of pramipexole and its impurities", volume = "65", number = "9-10", pages = "633-635", doi = "10.1365/s10337-007-0199-5" }
Jančić, B., Medenica, M., Ivanović, D.,& Malenović, A.. (2007). Chromatographic determination of dissociation constants of pramipexole and its impurities. in Chromatographia Vieweg, Wiesbaden., 65(9-10), 633-635. https://doi.org/10.1365/s10337-007-0199-5
Jančić B, Medenica M, Ivanović D, Malenović A. Chromatographic determination of dissociation constants of pramipexole and its impurities. in Chromatographia. 2007;65(9-10):633-635. doi:10.1365/s10337-007-0199-5 .
Jančić, Biljana, Medenica, Mirjana, Ivanović, D., Malenović, Anđelija, "Chromatographic determination of dissociation constants of pramipexole and its impurities" in Chromatographia, 65, no. 9-10 (2007):633-635, https://doi.org/10.1365/s10337-007-0199-5 . .