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dc.creatorCerović, Aleksandra
dc.creatorMiletić, Ivanka
dc.creatorŠobajić, Slađana
dc.creatorBlagojević, Duško
dc.creatorRadusinović, Miodrag
dc.creatorEl-Sohemy, Ahmed
dc.date.accessioned2019-09-02T11:09:37Z
dc.date.available2019-09-02T11:09:37Z
dc.date.issued2007
dc.identifier.issn0163-4984
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/927
dc.description.abstractZinc is an important mineral that is required for normal bone development. However, the direct effects of zinc on the mineralization of bone cells of human origin are not clear. The objective of this study was to determine the effects of zinc on the differentiation of SaOS-2 human osteoblast-like cells and the formation of mineralized bone nodules. Cells were cultured for 8 d and then transferred to zinc-free medium and treated with varying concentrations (0-50 mu M) of zinc. Alkaline phosphatase (ALP) activity was used as a measure of osteoblast differentiation, and bone nodules were detected by von Kossa staining. After 4, 6, and 8 d of treatment, zinc increased ALP activity at 1 and 10 mu M, but decreased activity at 50 mu M. After 9 d of treatment, zinc increased both the number and area of mineralized bone nodules at low concentrations (1 and 10 mu M), but decreased both at higher concentrations (25 and 50 mu M). These findings demonstrate that zinc has biphasic effects on the differentiation and mineralization of human osteoblast-like cells.en
dc.publisherHumana Press Inc, Totowa
dc.rightsrestrictedAccess
dc.sourceBiological Trace Element Research
dc.subjectzincen
dc.subjectosteoporosisen
dc.subjectSaOS-2 cellsen
dc.subjectboneen
dc.subjectalkaline phosphataseen
dc.titleEffects of zinc on the mineralization of bone nodules from human osteoblast-like cellsen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractРадусиновић, Миодраг; Ел-Сохемy, Aхмед; Милетић, Иванка; Благојевић, Душко; Шобајић, Слађана; Церовић, Aлександра;
dc.citation.volume116
dc.citation.issue1
dc.citation.spage61
dc.citation.epage71
dc.citation.other116(1): 61-71
dc.citation.rankM23
dc.identifier.wos000247177200006
dc.identifier.doi10.1007/BF02685919
dc.identifier.pmid17634628
dc.identifier.scopus2-s2.0-34547515972
dc.identifier.rcubconv_1860
dc.type.versionpublishedVersion


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