Kinetic determination of morphine by means of Bray-Liebhafsky oscillatory reaction system using analyte pulse perturbation technique
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Mijatović, Miroslav D.
Ćirić, Jasna S.
Marković, Zoran S.
Marković, Svetlana D.
Article (Published version)
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A novel kinetic method for micro-quantitative determinations of morphine (MH) is proposed and validated. The method is based on the potentiometric monitoring of the concentration perturbations of the oscillatory reaction system being in a stable non-equilibrium stationary state close to the bifurcation point between stable and oscillatory state. The response of the Bray-Liebhafsky (BL) oscillatory reaction as a matrix system, to the perturbations by different concentrations of morphine, is followed by a Pt-electrode. The proposed method relies on the linear relationship between maximal potential shift, Delta E-m, and the logarithm of the added morphine amounts in the range of 0.004-0.18 mu mol. Under optimum conditions, the sensitivity of the proposed method (as the limit of detection) is 0.001 mu mol and the method is featured by good precision (R.S.D. = 1.6%) as well as the excellent sample throughput (45 samples h(-1)). In addition to standard solution analysis, this approach was su...ccessfully applied for quantitative determination of morphine in a typical pharmaceutical dosage form. Some aspects of the possible mechanism of morphine action on the BL oscillating chemical system are discussed in detail.
Keywords:morphine / Bray-Liebhafsky reaction / quantitative analysis / analyte pulse perturbation / kinetic method
Source:Analytica Chimica Acta, 2007, 582, 2, 367-374
- Elsevier Science BV, Amsterdam