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Isocratic RP-HPLC method for rutin determination in solid oral dosage forms

Authorized Users Only
2007
Authors
Kuntić, Vesna
Pejić, Nataša
Ivković, Branka
Vujić, Zorica
Ilić, Katarina
Micić, Svetlana
Vukojević, Vladana
Article (Published version)
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Abstract
A rapid and sensitive assay for quantitative determination of rutin in oral dosage forms based on isocratic reversed phase high performance liquid chromatography (RP-HPLC) was developed and validated. Using a C-18 reverse-phase analytical column, the following conditions were chosen as optimal: mobile phase methanol-water 1:1 (v/v), pH 2.8 (adjusted with phosphoric acid), flow rate = 1 mL min(-1) and temperature T = 40.0 degrees C. Linearity was observed in the concentration range 8-120 mu g mL(-1) with a correlation coefficient of 0.99982 and the limit of detection (LOD) = 2.6 mu g mL(-1), and limit of quantification (LOQ) = 8.0 mu g mL(-1). Intra- and inter-day precision were within acceptable limits. Robustness test indicated that the mobile phase composition and pH influence mainly the separation. The proposed method allowed direct determination of rutin in pharmaceutical dosage forms in the presence of excipients, but is not suitable for preparations where compounds structurally/c...hemically related to rutin may be present.

Keywords:
rutin / isocratic RP-HPLC / pharmaceutical dosage forms
Source:
Journal of Pharmaceutical and Biomedical Analysis, 2007, 43, 2, 718-721
Publisher:
  • Pergamon-Elsevier Science Ltd, Oxford
Funding / projects:
  • Ispitivanje lekovitog potencijala biljaka: morfološka, hemijska i farmakološka karakterizacija (RS-143012)

DOI: 10.1016/j.jpba.2006.07.019

ISSN: 0731-7085

PubMed: 16920326

WoS: 000243802700045

Scopus: 2-s2.0-33845668735
[ Google Scholar ]
64
53
URI
https://farfar.pharmacy.bg.ac.rs/handle/123456789/944
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Pharmacy
TY  - JOUR
AU  - Kuntić, Vesna
AU  - Pejić, Nataša
AU  - Ivković, Branka
AU  - Vujić, Zorica
AU  - Ilić, Katarina
AU  - Micić, Svetlana
AU  - Vukojević, Vladana
PY  - 2007
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/944
AB  - A rapid and sensitive assay for quantitative determination of rutin in oral dosage forms based on isocratic reversed phase high performance liquid chromatography (RP-HPLC) was developed and validated. Using a C-18 reverse-phase analytical column, the following conditions were chosen as optimal: mobile phase methanol-water 1:1 (v/v), pH 2.8 (adjusted with phosphoric acid), flow rate = 1 mL min(-1) and temperature T = 40.0 degrees C. Linearity was observed in the concentration range 8-120 mu g mL(-1) with a correlation coefficient of 0.99982 and the limit of detection (LOD) = 2.6 mu g mL(-1), and limit of quantification (LOQ) = 8.0 mu g mL(-1). Intra- and inter-day precision were within acceptable limits. Robustness test indicated that the mobile phase composition and pH influence mainly the separation. The proposed method allowed direct determination of rutin in pharmaceutical dosage forms in the presence of excipients, but is not suitable for preparations where compounds structurally/chemically related to rutin may be present.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Isocratic RP-HPLC method for rutin determination in solid oral dosage forms
VL  - 43
IS  - 2
SP  - 718
EP  - 721
DO  - 10.1016/j.jpba.2006.07.019
ER  - 
@article{
author = "Kuntić, Vesna and Pejić, Nataša and Ivković, Branka and Vujić, Zorica and Ilić, Katarina and Micić, Svetlana and Vukojević, Vladana",
year = "2007",
abstract = "A rapid and sensitive assay for quantitative determination of rutin in oral dosage forms based on isocratic reversed phase high performance liquid chromatography (RP-HPLC) was developed and validated. Using a C-18 reverse-phase analytical column, the following conditions were chosen as optimal: mobile phase methanol-water 1:1 (v/v), pH 2.8 (adjusted with phosphoric acid), flow rate = 1 mL min(-1) and temperature T = 40.0 degrees C. Linearity was observed in the concentration range 8-120 mu g mL(-1) with a correlation coefficient of 0.99982 and the limit of detection (LOD) = 2.6 mu g mL(-1), and limit of quantification (LOQ) = 8.0 mu g mL(-1). Intra- and inter-day precision were within acceptable limits. Robustness test indicated that the mobile phase composition and pH influence mainly the separation. The proposed method allowed direct determination of rutin in pharmaceutical dosage forms in the presence of excipients, but is not suitable for preparations where compounds structurally/chemically related to rutin may be present.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Isocratic RP-HPLC method for rutin determination in solid oral dosage forms",
volume = "43",
number = "2",
pages = "718-721",
doi = "10.1016/j.jpba.2006.07.019"
}
Kuntić, V., Pejić, N., Ivković, B., Vujić, Z., Ilić, K., Micić, S.,& Vukojević, V.. (2007). Isocratic RP-HPLC method for rutin determination in solid oral dosage forms. in Journal of Pharmaceutical and Biomedical Analysis
Pergamon-Elsevier Science Ltd, Oxford., 43(2), 718-721.
https://doi.org/10.1016/j.jpba.2006.07.019
Kuntić V, Pejić N, Ivković B, Vujić Z, Ilić K, Micić S, Vukojević V. Isocratic RP-HPLC method for rutin determination in solid oral dosage forms. in Journal of Pharmaceutical and Biomedical Analysis. 2007;43(2):718-721.
doi:10.1016/j.jpba.2006.07.019 .
Kuntić, Vesna, Pejić, Nataša, Ivković, Branka, Vujić, Zorica, Ilić, Katarina, Micić, Svetlana, Vukojević, Vladana, "Isocratic RP-HPLC method for rutin determination in solid oral dosage forms" in Journal of Pharmaceutical and Biomedical Analysis, 43, no. 2 (2007):718-721,
https://doi.org/10.1016/j.jpba.2006.07.019 . .

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