Isocratic RP-HPLC method for rutin determination in solid oral dosage forms
Authorized Users Only
2007
Authors
Kuntić, VesnaPejić, Nataša

Ivković, Branka

Vujić, Zorica

Ilić, Katarina
Micić, Svetlana
Vukojević, Vladana

Article (Published version)

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A rapid and sensitive assay for quantitative determination of rutin in oral dosage forms based on isocratic reversed phase high performance liquid chromatography (RP-HPLC) was developed and validated. Using a C-18 reverse-phase analytical column, the following conditions were chosen as optimal: mobile phase methanol-water 1:1 (v/v), pH 2.8 (adjusted with phosphoric acid), flow rate = 1 mL min(-1) and temperature T = 40.0 degrees C. Linearity was observed in the concentration range 8-120 mu g mL(-1) with a correlation coefficient of 0.99982 and the limit of detection (LOD) = 2.6 mu g mL(-1), and limit of quantification (LOQ) = 8.0 mu g mL(-1). Intra- and inter-day precision were within acceptable limits. Robustness test indicated that the mobile phase composition and pH influence mainly the separation. The proposed method allowed direct determination of rutin in pharmaceutical dosage forms in the presence of excipients, but is not suitable for preparations where compounds structurally/c...hemically related to rutin may be present.
Keywords:
rutin / isocratic RP-HPLC / pharmaceutical dosage formsSource:
Journal of Pharmaceutical and Biomedical Analysis, 2007, 43, 2, 718-721Publisher:
- Pergamon-Elsevier Science Ltd, Oxford
Funding / projects:
- Ispitivanje lekovitog potencijala biljaka: morfološka, hemijska i farmakološka karakterizacija (RS-143012)
DOI: 10.1016/j.jpba.2006.07.019
ISSN: 0731-7085
PubMed: 16920326
WoS: 000243802700045
Scopus: 2-s2.0-33845668735
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Institution/Community
PharmacyTY - JOUR AU - Kuntić, Vesna AU - Pejić, Nataša AU - Ivković, Branka AU - Vujić, Zorica AU - Ilić, Katarina AU - Micić, Svetlana AU - Vukojević, Vladana PY - 2007 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/944 AB - A rapid and sensitive assay for quantitative determination of rutin in oral dosage forms based on isocratic reversed phase high performance liquid chromatography (RP-HPLC) was developed and validated. Using a C-18 reverse-phase analytical column, the following conditions were chosen as optimal: mobile phase methanol-water 1:1 (v/v), pH 2.8 (adjusted with phosphoric acid), flow rate = 1 mL min(-1) and temperature T = 40.0 degrees C. Linearity was observed in the concentration range 8-120 mu g mL(-1) with a correlation coefficient of 0.99982 and the limit of detection (LOD) = 2.6 mu g mL(-1), and limit of quantification (LOQ) = 8.0 mu g mL(-1). Intra- and inter-day precision were within acceptable limits. Robustness test indicated that the mobile phase composition and pH influence mainly the separation. The proposed method allowed direct determination of rutin in pharmaceutical dosage forms in the presence of excipients, but is not suitable for preparations where compounds structurally/chemically related to rutin may be present. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Journal of Pharmaceutical and Biomedical Analysis T1 - Isocratic RP-HPLC method for rutin determination in solid oral dosage forms VL - 43 IS - 2 SP - 718 EP - 721 DO - 10.1016/j.jpba.2006.07.019 ER -
@article{ author = "Kuntić, Vesna and Pejić, Nataša and Ivković, Branka and Vujić, Zorica and Ilić, Katarina and Micić, Svetlana and Vukojević, Vladana", year = "2007", abstract = "A rapid and sensitive assay for quantitative determination of rutin in oral dosage forms based on isocratic reversed phase high performance liquid chromatography (RP-HPLC) was developed and validated. Using a C-18 reverse-phase analytical column, the following conditions were chosen as optimal: mobile phase methanol-water 1:1 (v/v), pH 2.8 (adjusted with phosphoric acid), flow rate = 1 mL min(-1) and temperature T = 40.0 degrees C. Linearity was observed in the concentration range 8-120 mu g mL(-1) with a correlation coefficient of 0.99982 and the limit of detection (LOD) = 2.6 mu g mL(-1), and limit of quantification (LOQ) = 8.0 mu g mL(-1). Intra- and inter-day precision were within acceptable limits. Robustness test indicated that the mobile phase composition and pH influence mainly the separation. The proposed method allowed direct determination of rutin in pharmaceutical dosage forms in the presence of excipients, but is not suitable for preparations where compounds structurally/chemically related to rutin may be present.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Journal of Pharmaceutical and Biomedical Analysis", title = "Isocratic RP-HPLC method for rutin determination in solid oral dosage forms", volume = "43", number = "2", pages = "718-721", doi = "10.1016/j.jpba.2006.07.019" }
Kuntić, V., Pejić, N., Ivković, B., Vujić, Z., Ilić, K., Micić, S.,& Vukojević, V.. (2007). Isocratic RP-HPLC method for rutin determination in solid oral dosage forms. in Journal of Pharmaceutical and Biomedical Analysis Pergamon-Elsevier Science Ltd, Oxford., 43(2), 718-721. https://doi.org/10.1016/j.jpba.2006.07.019
Kuntić V, Pejić N, Ivković B, Vujić Z, Ilić K, Micić S, Vukojević V. Isocratic RP-HPLC method for rutin determination in solid oral dosage forms. in Journal of Pharmaceutical and Biomedical Analysis. 2007;43(2):718-721. doi:10.1016/j.jpba.2006.07.019 .
Kuntić, Vesna, Pejić, Nataša, Ivković, Branka, Vujić, Zorica, Ilić, Katarina, Micić, Svetlana, Vukojević, Vladana, "Isocratic RP-HPLC method for rutin determination in solid oral dosage forms" in Journal of Pharmaceutical and Biomedical Analysis, 43, no. 2 (2007):718-721, https://doi.org/10.1016/j.jpba.2006.07.019 . .