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dc.creatorSavić, G.
dc.creatorZečević, Mira
dc.creatorJocić, B.
dc.creatorŽivanović, Lj.
dc.date.accessioned2019-09-02T11:10:55Z
dc.date.available2019-09-02T11:10:55Z
dc.date.issued2007
dc.identifier.issn0009-5893
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/975
dc.description.abstractAn HPLC method has been developed for the separation of valdecoxib and a degradation product consisting of alpha and beta-N-lactosyl sulfonamide, i.e. alpha and beta anomers (SC-77852). Best results were achieved with a Chromolith Performance RP-18e column (100 mm x 4.6 mm), macropore size 2 pm, mesopore size 13 nm, with an eluent of methanol:water containing a 1% solution of TEA (36:64 v/v), pH 7.4 (adjusted with 85% orthophosphoric acid), at 22 degrees C. Detection was at 220 nm. The method was validated for its selectivity, linearity, precision (repeatability) and robustness. Quantitation and detection limits were determined for both valdecoxib and SC-77852. Method robustness was further evaluated by performing 2 3 full factorial design experiments. The final step, optimisation of the variables, was performed using response surface design. The validated method was used for assay of valdecoxib and SC77852 in Bextra((R)) film-coated tablets.en
dc.publisherVieweg, Wiesbaden
dc.rightsrestrictedAccess
dc.sourceChromatographia
dc.subjectcolumn liquid chromatographyen
dc.subjectrobustnessen
dc.subjectexperimental designen
dc.subjectvaldecoxib and its degradation product SC-77852en
dc.titleValidation of an HPLC method for the determination of valdecoxib and its degradation product: a mixture of alpha- and beta-n-lactosyl sulfonamide anomersen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractЈоцић, Б.; Савић, Г.; Живановић, Љ.; Зечевић, Мира;
dc.citation.volume66
dc.citation.issue1-2
dc.citation.spage29
dc.citation.epage35
dc.citation.other66(1-2): 29-35
dc.citation.rankM23
dc.identifier.wos000248715000006
dc.identifier.doi10.1365/s10337-007-0276-9
dc.identifier.scopus2-s2.0-34447095851
dc.identifier.rcubconv_1880
dc.type.versionpublishedVersion


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