Evidence that endothelium-dependent relaxation to histamine in the rat common carotid artery is mediated by EDRF

Abstract

The mechanism of histamine-induced relaxation in the isolated rat common carotid artery was analysed. Histamine (3 × 10-7 -10-4 mol/1) caused a concentration-dependent relaxation of the artery. In-domethacin, a cyclo-oxygenase inhibitor, and diethylcarbamazine, a lipoxygenase inhibitor, did not affect the relaxant response of the artery to histamine. After removal of the vascular endothelium the histamine-induced relaxation was strongly reduced. Moreover, hemoglobin and methylene blue, inhibitors of endothelium-derived relaxing factor (EDRF), prevented or reversed the relaxant effect of histamine. These findings confirm that the histamine-induced relaxation is to a greater extent endothelium dependent. It is concluded that the endothelium-dependent component in the relaxant response of the rat common carotid artery to histamine results from the release of EDRF.