Simultaneous determination of cefotaxime and desacetylcefotaxime in real urine sample using voltammetric and high-performance liquid chromatographic methods
Abstract
Two rapid, accurate and sensitive methods are developed and validated for the quantitative simultaneous determination of cefotaxime (CFX) and its active metabolite desacetylcefotaxime (DCFX) in urine. Based on the previous results which showed the four electron reduction of CFX at approximate to -0.5 V, and the new findings that DCFX reduction occurred at more positive potential (-0.23 V), the new adsorptive stripping differential pulse voltammetric (AdSDPV) method was developed for determination of CFX in the presence of DCFX. Linear responses were observed over a wide concentration range (0.07-0.52 mu g/ml for CFX and 0.22-1.3 mu g/ml for DCFX) in urine. The second assay involves subsequent separation on a reversed-phase HPLC column, with ultraviolet detection at 262 nm. Retention times were 4.057 and 1.960 min for CFX and DCFX, respectively. Linear responses were observed over a wide range, 0.55-6.60 mu g/ml for CFX and 1.10-11.00 mu g/ml for DCFX, in urine. The statistical evaluati...on for both methods was examined by means of within-day repeatability (n=5) and day-to-day precision (n=3) and was found to be satisfactory with high accuracy and precision.
Keywords:
Cefotaxime / Desacetylcefotaxime / Simultaneous determination / Real urine / Voltammetry / RP-HPLCSource:
Talanta, 2008, 77, 1, 131-137Publisher:
- Elsevier Science BV, Amsterdam
Funding / projects:
- Sinteza, kvantitativni odnosi između strukture/osobina i aktivnosti, fizičko-hemijska karakterizacija i analiza farmakološki aktivnih supstanci (RS-MESTD-MPN2006-2010-142071)
DOI: 10.1016/j.talanta.2008.05.047
ISSN: 0039-9140
PubMed: 18804610
WoS: 000260290200020
Scopus: 2-s2.0-51749107978
Collections
Institution/Community
PharmacyTY - JOUR AU - Aleksić, Mara AU - Kapetanović, Vera AU - Atanacković, Jasmina AU - Jocić, Biljana AU - Zečević, Mira PY - 2008 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1032 AB - Two rapid, accurate and sensitive methods are developed and validated for the quantitative simultaneous determination of cefotaxime (CFX) and its active metabolite desacetylcefotaxime (DCFX) in urine. Based on the previous results which showed the four electron reduction of CFX at approximate to -0.5 V, and the new findings that DCFX reduction occurred at more positive potential (-0.23 V), the new adsorptive stripping differential pulse voltammetric (AdSDPV) method was developed for determination of CFX in the presence of DCFX. Linear responses were observed over a wide concentration range (0.07-0.52 mu g/ml for CFX and 0.22-1.3 mu g/ml for DCFX) in urine. The second assay involves subsequent separation on a reversed-phase HPLC column, with ultraviolet detection at 262 nm. Retention times were 4.057 and 1.960 min for CFX and DCFX, respectively. Linear responses were observed over a wide range, 0.55-6.60 mu g/ml for CFX and 1.10-11.00 mu g/ml for DCFX, in urine. The statistical evaluation for both methods was examined by means of within-day repeatability (n=5) and day-to-day precision (n=3) and was found to be satisfactory with high accuracy and precision. PB - Elsevier Science BV, Amsterdam T2 - Talanta T1 - Simultaneous determination of cefotaxime and desacetylcefotaxime in real urine sample using voltammetric and high-performance liquid chromatographic methods VL - 77 IS - 1 SP - 131 EP - 137 DO - 10.1016/j.talanta.2008.05.047 ER -
@article{ author = "Aleksić, Mara and Kapetanović, Vera and Atanacković, Jasmina and Jocić, Biljana and Zečević, Mira", year = "2008", abstract = "Two rapid, accurate and sensitive methods are developed and validated for the quantitative simultaneous determination of cefotaxime (CFX) and its active metabolite desacetylcefotaxime (DCFX) in urine. Based on the previous results which showed the four electron reduction of CFX at approximate to -0.5 V, and the new findings that DCFX reduction occurred at more positive potential (-0.23 V), the new adsorptive stripping differential pulse voltammetric (AdSDPV) method was developed for determination of CFX in the presence of DCFX. Linear responses were observed over a wide concentration range (0.07-0.52 mu g/ml for CFX and 0.22-1.3 mu g/ml for DCFX) in urine. The second assay involves subsequent separation on a reversed-phase HPLC column, with ultraviolet detection at 262 nm. Retention times were 4.057 and 1.960 min for CFX and DCFX, respectively. Linear responses were observed over a wide range, 0.55-6.60 mu g/ml for CFX and 1.10-11.00 mu g/ml for DCFX, in urine. The statistical evaluation for both methods was examined by means of within-day repeatability (n=5) and day-to-day precision (n=3) and was found to be satisfactory with high accuracy and precision.", publisher = "Elsevier Science BV, Amsterdam", journal = "Talanta", title = "Simultaneous determination of cefotaxime and desacetylcefotaxime in real urine sample using voltammetric and high-performance liquid chromatographic methods", volume = "77", number = "1", pages = "131-137", doi = "10.1016/j.talanta.2008.05.047" }
Aleksić, M., Kapetanović, V., Atanacković, J., Jocić, B.,& Zečević, M.. (2008). Simultaneous determination of cefotaxime and desacetylcefotaxime in real urine sample using voltammetric and high-performance liquid chromatographic methods. in Talanta Elsevier Science BV, Amsterdam., 77(1), 131-137. https://doi.org/10.1016/j.talanta.2008.05.047
Aleksić M, Kapetanović V, Atanacković J, Jocić B, Zečević M. Simultaneous determination of cefotaxime and desacetylcefotaxime in real urine sample using voltammetric and high-performance liquid chromatographic methods. in Talanta. 2008;77(1):131-137. doi:10.1016/j.talanta.2008.05.047 .
Aleksić, Mara, Kapetanović, Vera, Atanacković, Jasmina, Jocić, Biljana, Zečević, Mira, "Simultaneous determination of cefotaxime and desacetylcefotaxime in real urine sample using voltammetric and high-performance liquid chromatographic methods" in Talanta, 77, no. 1 (2008):131-137, https://doi.org/10.1016/j.talanta.2008.05.047 . .