A role of ion channels in the endothelium-independent relaxation of rat mesenteric artery induced by resveratrol
2008
Аутори
Gojković-Bukarica, LjiljanaNovaković, Aleksandra
Kanjuh, Vladimir
Bumbasirević, Marko
Lesić, Aleksandar
Heinle, Helmut
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Recently it has been suggested that resveratrol relaxes different isolated arteries. The present study addressed the question whether different ion channels are involved in the endothelium-independent mechanism of vasodilatation induced by resveratrol. For that purpose, we tested the action of resveratrol on the rat mesenteric artery without endothelium. Resveratrol induced con centration-dependent relaxation of rat mesenteric artery. Among the K+-channel blockers, 4-amynopiridine (4-AP) moderately antagonized the resveratrol-induced relaxation, while glibendamide, tetraethylammonium chloride, charybdotoxin, margatoxin, and barium chloride did not inhibit resveratrol-induced vasorelaxation. In rings, precontracted with 100 mM K+, the relaxant responses to resveratrol were highly significantly shifted to the right compared to those obtained in rings precontracted with phenylephrine, but resveratrol-induced maximal relaxation was only slightly affected. In order to minimize the influence... of K+ channels and voltage-gated Ca2+ channels (VGCCs) in vascular smooth muscle, the third contraction was made by 100 in M K- in the presence of nifedipine. The relaxant response to resveratrol was abolished. Thus, the mechanism of vasorelaxation induced by resveratrol probably involves activation of 4-AP-sensitive K+ channels. Its ability to completely relax the mesenteric artery precontracted with K+-rich solution suggests that K channel-independent mechanism(s) are involved in its vasorelaxant effect. It seems that interaction with VGCCs plays a part in this K+ channel-independent effect of resveratrol.
Кључне речи:
resveratrol / rat mesenteric artery / ion channel / vasorelaxation / K+ channelИзвор:
Journal of Pharmacological Sciences, 2008, 108, 1, 124-130Издавач:
- Japanese Pharmacological Soc, Kyoto
Финансирање / пројекти:
- Ministry of Science and Technology (Serbia)
- Karl & Lora Klein Foundation (Germany)
DOI: 10.1254/jphs.08128FP
ISSN: 1347-8613
PubMed: 18818483
WoS: 000259683400015
Scopus: 2-s2.0-52949145570
Институција/група
PharmacyTY - JOUR AU - Gojković-Bukarica, Ljiljana AU - Novaković, Aleksandra AU - Kanjuh, Vladimir AU - Bumbasirević, Marko AU - Lesić, Aleksandar AU - Heinle, Helmut PY - 2008 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1041 AB - Recently it has been suggested that resveratrol relaxes different isolated arteries. The present study addressed the question whether different ion channels are involved in the endothelium-independent mechanism of vasodilatation induced by resveratrol. For that purpose, we tested the action of resveratrol on the rat mesenteric artery without endothelium. Resveratrol induced con centration-dependent relaxation of rat mesenteric artery. Among the K+-channel blockers, 4-amynopiridine (4-AP) moderately antagonized the resveratrol-induced relaxation, while glibendamide, tetraethylammonium chloride, charybdotoxin, margatoxin, and barium chloride did not inhibit resveratrol-induced vasorelaxation. In rings, precontracted with 100 mM K+, the relaxant responses to resveratrol were highly significantly shifted to the right compared to those obtained in rings precontracted with phenylephrine, but resveratrol-induced maximal relaxation was only slightly affected. In order to minimize the influence of K+ channels and voltage-gated Ca2+ channels (VGCCs) in vascular smooth muscle, the third contraction was made by 100 in M K- in the presence of nifedipine. The relaxant response to resveratrol was abolished. Thus, the mechanism of vasorelaxation induced by resveratrol probably involves activation of 4-AP-sensitive K+ channels. Its ability to completely relax the mesenteric artery precontracted with K+-rich solution suggests that K channel-independent mechanism(s) are involved in its vasorelaxant effect. It seems that interaction with VGCCs plays a part in this K+ channel-independent effect of resveratrol. PB - Japanese Pharmacological Soc, Kyoto T2 - Journal of Pharmacological Sciences T1 - A role of ion channels in the endothelium-independent relaxation of rat mesenteric artery induced by resveratrol VL - 108 IS - 1 SP - 124 EP - 130 DO - 10.1254/jphs.08128FP ER -
@article{ author = "Gojković-Bukarica, Ljiljana and Novaković, Aleksandra and Kanjuh, Vladimir and Bumbasirević, Marko and Lesić, Aleksandar and Heinle, Helmut", year = "2008", abstract = "Recently it has been suggested that resveratrol relaxes different isolated arteries. The present study addressed the question whether different ion channels are involved in the endothelium-independent mechanism of vasodilatation induced by resveratrol. For that purpose, we tested the action of resveratrol on the rat mesenteric artery without endothelium. Resveratrol induced con centration-dependent relaxation of rat mesenteric artery. Among the K+-channel blockers, 4-amynopiridine (4-AP) moderately antagonized the resveratrol-induced relaxation, while glibendamide, tetraethylammonium chloride, charybdotoxin, margatoxin, and barium chloride did not inhibit resveratrol-induced vasorelaxation. In rings, precontracted with 100 mM K+, the relaxant responses to resveratrol were highly significantly shifted to the right compared to those obtained in rings precontracted with phenylephrine, but resveratrol-induced maximal relaxation was only slightly affected. In order to minimize the influence of K+ channels and voltage-gated Ca2+ channels (VGCCs) in vascular smooth muscle, the third contraction was made by 100 in M K- in the presence of nifedipine. The relaxant response to resveratrol was abolished. Thus, the mechanism of vasorelaxation induced by resveratrol probably involves activation of 4-AP-sensitive K+ channels. Its ability to completely relax the mesenteric artery precontracted with K+-rich solution suggests that K channel-independent mechanism(s) are involved in its vasorelaxant effect. It seems that interaction with VGCCs plays a part in this K+ channel-independent effect of resveratrol.", publisher = "Japanese Pharmacological Soc, Kyoto", journal = "Journal of Pharmacological Sciences", title = "A role of ion channels in the endothelium-independent relaxation of rat mesenteric artery induced by resveratrol", volume = "108", number = "1", pages = "124-130", doi = "10.1254/jphs.08128FP" }
Gojković-Bukarica, L., Novaković, A., Kanjuh, V., Bumbasirević, M., Lesić, A.,& Heinle, H.. (2008). A role of ion channels in the endothelium-independent relaxation of rat mesenteric artery induced by resveratrol. in Journal of Pharmacological Sciences Japanese Pharmacological Soc, Kyoto., 108(1), 124-130. https://doi.org/10.1254/jphs.08128FP
Gojković-Bukarica L, Novaković A, Kanjuh V, Bumbasirević M, Lesić A, Heinle H. A role of ion channels in the endothelium-independent relaxation of rat mesenteric artery induced by resveratrol. in Journal of Pharmacological Sciences. 2008;108(1):124-130. doi:10.1254/jphs.08128FP .
Gojković-Bukarica, Ljiljana, Novaković, Aleksandra, Kanjuh, Vladimir, Bumbasirević, Marko, Lesić, Aleksandar, Heinle, Helmut, "A role of ion channels in the endothelium-independent relaxation of rat mesenteric artery induced by resveratrol" in Journal of Pharmacological Sciences, 108, no. 1 (2008):124-130, https://doi.org/10.1254/jphs.08128FP . .